Hydrolysis Process Optimization and Functional Characterization of Yak Skin Gelatin Hydrolysates
Yak (Bos grunniens) is an animal mainly living on the Tibetan Plateau. Yak skin is a valuable resource that is wasted in the meat production process. This study aimed to prepare yak skin gelatin hydrolysates (YSGH) from yak skin through enzymatic hydrolysis and investigate functional characterizatio...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2019/9105605 |
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| author | Hui Yang Yanting Xue Jiaheng Liu Shunyi Song Lei Zhang Qianqian Song Li Tian Xiangyu He Shan He Hongji Zhu |
| author_facet | Hui Yang Yanting Xue Jiaheng Liu Shunyi Song Lei Zhang Qianqian Song Li Tian Xiangyu He Shan He Hongji Zhu |
| author_sort | Hui Yang |
| collection | DOAJ |
| description | Yak (Bos grunniens) is an animal mainly living on the Tibetan Plateau. Yak skin is a valuable resource that is wasted in the meat production process. This study aimed to prepare yak skin gelatin hydrolysates (YSGH) from yak skin through enzymatic hydrolysis and investigate functional characterization of YSGH. We showed that trypsin was more effective than neutrase, papain, and pepsin in increasing the degree of hydrolysis (DH) of YSGH. The conditions of enzymatic hydrolysis were optimized using central composite design (CCD) and response surface method (RSM), and the highest DH value of 31.96% was obtained. We then analyzed the amino acid compositions and molecular weight distribution of peptides in YSGH. The obtained YSGH exhibited certain antioxidant activity and excellent ACE-inhibitory activity (IC50 = 0.991 mg/mL). In addition, the solubility (98.79%), emulsification, and foaming properties of YSGH developed here were also evaluated. With these physicochemical and biological functions, YSGH had potential applications in food, pharmaceuticals, and cosmetics as an ingredient. |
| format | Article |
| id | doaj-art-77a3779ea1fd4d1d91ac6dba8d77f4a3 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-77a3779ea1fd4d1d91ac6dba8d77f4a32025-08-20T03:35:38ZengWileyJournal of Chemistry2090-90632090-90712019-01-01201910.1155/2019/91056059105605Hydrolysis Process Optimization and Functional Characterization of Yak Skin Gelatin HydrolysatesHui Yang0Yanting Xue1Jiaheng Liu2Shunyi Song3Lei Zhang4Qianqian Song5Li Tian6Xiangyu He7Shan He8Hongji Zhu9Key Laboratory of Systems Bioengineering, Ministry of Education (Tianjin University), Tianjin 300072, ChinaKey Laboratory of Systems Bioengineering, Ministry of Education (Tianjin University), Tianjin 300072, ChinaKey Laboratory of Systems Bioengineering, Ministry of Education (Tianjin University), Tianjin 300072, ChinaKey Laboratory of Systems Bioengineering, Ministry of Education (Tianjin University), Tianjin 300072, ChinaKey Laboratory of Systems Bioengineering, Ministry of Education (Tianjin University), Tianjin 300072, ChinaKey Laboratory of Systems Bioengineering, Ministry of Education (Tianjin University), Tianjin 300072, ChinaKey Laboratory of Systems Bioengineering, Ministry of Education (Tianjin University), Tianjin 300072, ChinaKey Laboratory of Systems Bioengineering, Ministry of Education (Tianjin University), Tianjin 300072, ChinaSchool of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou, ChinaKey Laboratory of Systems Bioengineering, Ministry of Education (Tianjin University), Tianjin 300072, ChinaYak (Bos grunniens) is an animal mainly living on the Tibetan Plateau. Yak skin is a valuable resource that is wasted in the meat production process. This study aimed to prepare yak skin gelatin hydrolysates (YSGH) from yak skin through enzymatic hydrolysis and investigate functional characterization of YSGH. We showed that trypsin was more effective than neutrase, papain, and pepsin in increasing the degree of hydrolysis (DH) of YSGH. The conditions of enzymatic hydrolysis were optimized using central composite design (CCD) and response surface method (RSM), and the highest DH value of 31.96% was obtained. We then analyzed the amino acid compositions and molecular weight distribution of peptides in YSGH. The obtained YSGH exhibited certain antioxidant activity and excellent ACE-inhibitory activity (IC50 = 0.991 mg/mL). In addition, the solubility (98.79%), emulsification, and foaming properties of YSGH developed here were also evaluated. With these physicochemical and biological functions, YSGH had potential applications in food, pharmaceuticals, and cosmetics as an ingredient.http://dx.doi.org/10.1155/2019/9105605 |
| spellingShingle | Hui Yang Yanting Xue Jiaheng Liu Shunyi Song Lei Zhang Qianqian Song Li Tian Xiangyu He Shan He Hongji Zhu Hydrolysis Process Optimization and Functional Characterization of Yak Skin Gelatin Hydrolysates Journal of Chemistry |
| title | Hydrolysis Process Optimization and Functional Characterization of Yak Skin Gelatin Hydrolysates |
| title_full | Hydrolysis Process Optimization and Functional Characterization of Yak Skin Gelatin Hydrolysates |
| title_fullStr | Hydrolysis Process Optimization and Functional Characterization of Yak Skin Gelatin Hydrolysates |
| title_full_unstemmed | Hydrolysis Process Optimization and Functional Characterization of Yak Skin Gelatin Hydrolysates |
| title_short | Hydrolysis Process Optimization and Functional Characterization of Yak Skin Gelatin Hydrolysates |
| title_sort | hydrolysis process optimization and functional characterization of yak skin gelatin hydrolysates |
| url | http://dx.doi.org/10.1155/2019/9105605 |
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