Enabling rapid parallel SERS detection with integrated microlens array

Surface-enhanced Raman Spectroscopy (SERS) is a highly sensitive analytical tool with great potential for point-of-care diagnostics. However, integrating SERS with microfluidic chips poses challenges, including low signal uniformity, low light collection efficiency, and high sensitivity to optical m...

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Main Authors: Sara Abbasi, Qing Liu, Dries Rosseel, Hugo Thienpont, Heidi Ottevaere
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Sensing and Bio-Sensing Research
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Online Access:http://www.sciencedirect.com/science/article/pii/S2214180425000741
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author Sara Abbasi
Qing Liu
Dries Rosseel
Hugo Thienpont
Heidi Ottevaere
author_facet Sara Abbasi
Qing Liu
Dries Rosseel
Hugo Thienpont
Heidi Ottevaere
author_sort Sara Abbasi
collection DOAJ
description Surface-enhanced Raman Spectroscopy (SERS) is a highly sensitive analytical tool with great potential for point-of-care diagnostics. However, integrating SERS with microfluidic chips poses challenges, including low signal uniformity, low light collection efficiency, and high sensitivity to optical misalignment due to the precise focusing required within microfluidic channels. This study aims to overcome these limitations by integrating a high-optical-quality Microlens Array (MLA) into a SERS-embedded platform chip. The MLA focuses light into distinct excitation points, enabling parallel Raman scattering collection and improving overall collection efficiency while reducing sensitivity to alignment issues. Integrating the MLA into the SERS platform resulted in a rise in power density from 40 W/cm2 to 700 W/cm2, leading to a fivefold improvement in the signal-to-noise (SNR) ratio of the collected Raman signal intensity. The MLA's multiplexing capability enabled the simultaneous excitation and detection of multiple analyte regions, providing high signal collection in a single run. These findings highlight the MLA-integrated SERS platform as a scalable, high-sensitivity platform for point-of-care diagnostics, real-time analysis, and high-throughput sensing, addressing critical challenges in light delivery and optical alignment demonstrated low sensitivity to misalignments in the x, y, and z axes.
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series Sensing and Bio-Sensing Research
spelling doaj-art-779fa59af0ca4560924e88bba03e0a282025-08-20T02:07:40ZengElsevierSensing and Bio-Sensing Research2214-18042025-06-014810080810.1016/j.sbsr.2025.100808Enabling rapid parallel SERS detection with integrated microlens arraySara Abbasi0Qing Liu1Dries Rosseel2Hugo Thienpont3Heidi Ottevaere4Vrije Universiteit Brussel, Department of Applied Physics and Photonics, Brussels Photonics, Pleinlaan 2, 1050 Brussel, Belgium; Corresponding author.Vrije Universiteit Brussel, Department of Applied Physics and Photonics, Brussels Photonics, Pleinlaan 2, 1050 Brussel, Belgium; Flanders Make@VUB - BP&M, Pleinlaan 2, 1050 Brussel, BelgiumVrije Universiteit Brussel, Department of Applied Physics and Photonics, Brussels Photonics, Pleinlaan 2, 1050 Brussel, Belgium; Flanders Make@VUB - BP&M, Pleinlaan 2, 1050 Brussel, BelgiumVrije Universiteit Brussel, Department of Applied Physics and Photonics, Brussels Photonics, Pleinlaan 2, 1050 Brussel, Belgium; Flanders Make@VUB - BP&M, Pleinlaan 2, 1050 Brussel, BelgiumVrije Universiteit Brussel, Department of Applied Physics and Photonics, Brussels Photonics, Pleinlaan 2, 1050 Brussel, Belgium; Flanders Make@VUB - BP&M, Pleinlaan 2, 1050 Brussel, BelgiumSurface-enhanced Raman Spectroscopy (SERS) is a highly sensitive analytical tool with great potential for point-of-care diagnostics. However, integrating SERS with microfluidic chips poses challenges, including low signal uniformity, low light collection efficiency, and high sensitivity to optical misalignment due to the precise focusing required within microfluidic channels. This study aims to overcome these limitations by integrating a high-optical-quality Microlens Array (MLA) into a SERS-embedded platform chip. The MLA focuses light into distinct excitation points, enabling parallel Raman scattering collection and improving overall collection efficiency while reducing sensitivity to alignment issues. Integrating the MLA into the SERS platform resulted in a rise in power density from 40 W/cm2 to 700 W/cm2, leading to a fivefold improvement in the signal-to-noise (SNR) ratio of the collected Raman signal intensity. The MLA's multiplexing capability enabled the simultaneous excitation and detection of multiple analyte regions, providing high signal collection in a single run. These findings highlight the MLA-integrated SERS platform as a scalable, high-sensitivity platform for point-of-care diagnostics, real-time analysis, and high-throughput sensing, addressing critical challenges in light delivery and optical alignment demonstrated low sensitivity to misalignments in the x, y, and z axes.http://www.sciencedirect.com/science/article/pii/S2214180425000741Surface-enhanced Raman spectroscopyMicrolens arrayMicrofluidic chipMultiplexingPoint-of-care diagnosticsImproving SERS
spellingShingle Sara Abbasi
Qing Liu
Dries Rosseel
Hugo Thienpont
Heidi Ottevaere
Enabling rapid parallel SERS detection with integrated microlens array
Sensing and Bio-Sensing Research
Surface-enhanced Raman spectroscopy
Microlens array
Microfluidic chip
Multiplexing
Point-of-care diagnostics
Improving SERS
title Enabling rapid parallel SERS detection with integrated microlens array
title_full Enabling rapid parallel SERS detection with integrated microlens array
title_fullStr Enabling rapid parallel SERS detection with integrated microlens array
title_full_unstemmed Enabling rapid parallel SERS detection with integrated microlens array
title_short Enabling rapid parallel SERS detection with integrated microlens array
title_sort enabling rapid parallel sers detection with integrated microlens array
topic Surface-enhanced Raman spectroscopy
Microlens array
Microfluidic chip
Multiplexing
Point-of-care diagnostics
Improving SERS
url http://www.sciencedirect.com/science/article/pii/S2214180425000741
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AT hugothienpont enablingrapidparallelsersdetectionwithintegratedmicrolensarray
AT heidiottevaere enablingrapidparallelsersdetectionwithintegratedmicrolensarray