Codon-Driven Translational Efficiency Is Stable across Diverse Mammalian Cell States.
Whether codon usage fine-tunes mRNA translation in mammals remains controversial, with recent papers suggesting that production of proteins in specific Gene Ontological (GO) pathways can be regulated by actively modifying the codon and anticodon pools in different cellular conditions. In this work,...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-05-01
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| Series: | PLoS Genetics |
| Online Access: | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1006024&type=printable |
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| author | Konrad L M Rudolph Bianca M Schmitt Diego Villar Robert J White John C Marioni Claudia Kutter Duncan T Odom |
| author_facet | Konrad L M Rudolph Bianca M Schmitt Diego Villar Robert J White John C Marioni Claudia Kutter Duncan T Odom |
| author_sort | Konrad L M Rudolph |
| collection | DOAJ |
| description | Whether codon usage fine-tunes mRNA translation in mammals remains controversial, with recent papers suggesting that production of proteins in specific Gene Ontological (GO) pathways can be regulated by actively modifying the codon and anticodon pools in different cellular conditions. In this work, we compared the sequence content of genes in specific GO categories with the exonic genome background. Although a substantial fraction of variability in codon usage could be explained by random sampling, almost half of GO sets showed more variability in codon usage than expected by chance. Nevertheless, by quantifying translational efficiency in healthy and cancerous tissues in human and mouse, we demonstrated that a given tRNA pool can equally well translate many different sets of mRNAs, irrespective of their cell-type specificity. This disconnect between variations in codon usage and the stability of translational efficiency is best explained by differences in GC content between gene sets. GC variation across the mammalian genome is most likely a result of the interplay between genome repair and gene duplication mechanisms, rather than selective pressures caused by codon-driven translational rates. Consequently, codon usage differences in mammalian transcriptomes are most easily explained by well-understood mutational biases acting on the underlying genome. |
| format | Article |
| id | doaj-art-779b25bbe2bb4d289cd3281bdcc0c184 |
| institution | OA Journals |
| issn | 1553-7390 1553-7404 |
| language | English |
| publishDate | 2016-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj-art-779b25bbe2bb4d289cd3281bdcc0c1842025-08-20T02:31:59ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042016-05-01125e100602410.1371/journal.pgen.1006024Codon-Driven Translational Efficiency Is Stable across Diverse Mammalian Cell States.Konrad L M RudolphBianca M SchmittDiego VillarRobert J WhiteJohn C MarioniClaudia KutterDuncan T OdomWhether codon usage fine-tunes mRNA translation in mammals remains controversial, with recent papers suggesting that production of proteins in specific Gene Ontological (GO) pathways can be regulated by actively modifying the codon and anticodon pools in different cellular conditions. In this work, we compared the sequence content of genes in specific GO categories with the exonic genome background. Although a substantial fraction of variability in codon usage could be explained by random sampling, almost half of GO sets showed more variability in codon usage than expected by chance. Nevertheless, by quantifying translational efficiency in healthy and cancerous tissues in human and mouse, we demonstrated that a given tRNA pool can equally well translate many different sets of mRNAs, irrespective of their cell-type specificity. This disconnect between variations in codon usage and the stability of translational efficiency is best explained by differences in GC content between gene sets. GC variation across the mammalian genome is most likely a result of the interplay between genome repair and gene duplication mechanisms, rather than selective pressures caused by codon-driven translational rates. Consequently, codon usage differences in mammalian transcriptomes are most easily explained by well-understood mutational biases acting on the underlying genome.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1006024&type=printable |
| spellingShingle | Konrad L M Rudolph Bianca M Schmitt Diego Villar Robert J White John C Marioni Claudia Kutter Duncan T Odom Codon-Driven Translational Efficiency Is Stable across Diverse Mammalian Cell States. PLoS Genetics |
| title | Codon-Driven Translational Efficiency Is Stable across Diverse Mammalian Cell States. |
| title_full | Codon-Driven Translational Efficiency Is Stable across Diverse Mammalian Cell States. |
| title_fullStr | Codon-Driven Translational Efficiency Is Stable across Diverse Mammalian Cell States. |
| title_full_unstemmed | Codon-Driven Translational Efficiency Is Stable across Diverse Mammalian Cell States. |
| title_short | Codon-Driven Translational Efficiency Is Stable across Diverse Mammalian Cell States. |
| title_sort | codon driven translational efficiency is stable across diverse mammalian cell states |
| url | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1006024&type=printable |
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