Profiling of glycan receptors for minute virus of mice in permissive cell lines towards understanding the mechanism of cell recognition.
The recognition of sialic acids by two strains of minute virus of mice (MVM), MVMp (prototype) and MVMi (immunosuppressive), is an essential requirement for successful infection. To understand the potential for recognition of different modifications of sialic acid by MVM, three types of capsids, vir...
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0086909&type=printable |
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| author | Sujata Halder Susan Cotmore Jamie Heimburg-Molinaro David F Smith Richard D Cummings Xi Chen Alana J Trollope Simon J North Stuart M Haslam Anne Dell Peter Tattersall Robert McKenna Mavis Agbandje-McKenna |
| author_facet | Sujata Halder Susan Cotmore Jamie Heimburg-Molinaro David F Smith Richard D Cummings Xi Chen Alana J Trollope Simon J North Stuart M Haslam Anne Dell Peter Tattersall Robert McKenna Mavis Agbandje-McKenna |
| author_sort | Sujata Halder |
| collection | DOAJ |
| description | The recognition of sialic acids by two strains of minute virus of mice (MVM), MVMp (prototype) and MVMi (immunosuppressive), is an essential requirement for successful infection. To understand the potential for recognition of different modifications of sialic acid by MVM, three types of capsids, virus-like particles, wild type empty (no DNA) capsids, and DNA packaged virions, were screened on a sialylated glycan microarray (SGM). Both viruses demonstrated a preference for binding to 9-O-methylated sialic acid derivatives, while MVMp showed additional binding to 9-O-acetylated and 9-O-lactoylated sialic acid derivatives, indicating recognition differences. The glycans recognized contained a type-2 Galβ1-4GlcNAc motif (Neu5Acα2-3Galβ1-4GlcNAc or 3'SIA-LN) and were biantennary complex-type N-glycans with the exception of one. To correlate the recognition of the 3'SIA-LN glycan motif as well as the biantennary structures to their natural expression in cell lines permissive for MVMp, MVMi, or both strains, the N- and O-glycans, and polar glycolipids present in three cell lines used for in vitro studies, A9 fibroblasts, EL4 T lymphocytes, and the SV40 transformed NB324K cells, were analyzed by MALDI-TOF/TOF mass spectrometry. The cells showed an abundance of the sialylated glycan motifs recognized by the viruses in the SGM and previous glycan microarrays supporting their role in cellular recognition by MVM. Significantly, the NB324K showed fucosylation at the non-reducing end of their biantennary glycans, suggesting that recognition of these cells is possibly mediated by the Lewis X motif as in 3'SIA-Le(X) identified in a previous glycan microarray screen. |
| format | Article |
| id | doaj-art-779924ed2aa04d5682f8a67e85395aa3 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-779924ed2aa04d5682f8a67e85395aa32025-08-20T03:46:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0191e8690910.1371/journal.pone.0086909Profiling of glycan receptors for minute virus of mice in permissive cell lines towards understanding the mechanism of cell recognition.Sujata HalderSusan CotmoreJamie Heimburg-MolinaroDavid F SmithRichard D CummingsXi ChenAlana J TrollopeSimon J NorthStuart M HaslamAnne DellPeter TattersallRobert McKennaMavis Agbandje-McKennaThe recognition of sialic acids by two strains of minute virus of mice (MVM), MVMp (prototype) and MVMi (immunosuppressive), is an essential requirement for successful infection. To understand the potential for recognition of different modifications of sialic acid by MVM, three types of capsids, virus-like particles, wild type empty (no DNA) capsids, and DNA packaged virions, were screened on a sialylated glycan microarray (SGM). Both viruses demonstrated a preference for binding to 9-O-methylated sialic acid derivatives, while MVMp showed additional binding to 9-O-acetylated and 9-O-lactoylated sialic acid derivatives, indicating recognition differences. The glycans recognized contained a type-2 Galβ1-4GlcNAc motif (Neu5Acα2-3Galβ1-4GlcNAc or 3'SIA-LN) and were biantennary complex-type N-glycans with the exception of one. To correlate the recognition of the 3'SIA-LN glycan motif as well as the biantennary structures to their natural expression in cell lines permissive for MVMp, MVMi, or both strains, the N- and O-glycans, and polar glycolipids present in three cell lines used for in vitro studies, A9 fibroblasts, EL4 T lymphocytes, and the SV40 transformed NB324K cells, were analyzed by MALDI-TOF/TOF mass spectrometry. The cells showed an abundance of the sialylated glycan motifs recognized by the viruses in the SGM and previous glycan microarrays supporting their role in cellular recognition by MVM. Significantly, the NB324K showed fucosylation at the non-reducing end of their biantennary glycans, suggesting that recognition of these cells is possibly mediated by the Lewis X motif as in 3'SIA-Le(X) identified in a previous glycan microarray screen.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0086909&type=printable |
| spellingShingle | Sujata Halder Susan Cotmore Jamie Heimburg-Molinaro David F Smith Richard D Cummings Xi Chen Alana J Trollope Simon J North Stuart M Haslam Anne Dell Peter Tattersall Robert McKenna Mavis Agbandje-McKenna Profiling of glycan receptors for minute virus of mice in permissive cell lines towards understanding the mechanism of cell recognition. PLoS ONE |
| title | Profiling of glycan receptors for minute virus of mice in permissive cell lines towards understanding the mechanism of cell recognition. |
| title_full | Profiling of glycan receptors for minute virus of mice in permissive cell lines towards understanding the mechanism of cell recognition. |
| title_fullStr | Profiling of glycan receptors for minute virus of mice in permissive cell lines towards understanding the mechanism of cell recognition. |
| title_full_unstemmed | Profiling of glycan receptors for minute virus of mice in permissive cell lines towards understanding the mechanism of cell recognition. |
| title_short | Profiling of glycan receptors for minute virus of mice in permissive cell lines towards understanding the mechanism of cell recognition. |
| title_sort | profiling of glycan receptors for minute virus of mice in permissive cell lines towards understanding the mechanism of cell recognition |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0086909&type=printable |
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