miR-142-3p Contributes to Early Cardiac Fate Decision of Embryonic Stem Cells
MicroRNAs (miRNAs) play important roles in cell fate decisions. However, the miRNAs and their targets involved in the regulation of cardiac lineage specification are largely unexplored. Here, we report novel functions of miR-142-3p in the regulation of cardiomyocyte differentiation from mouse embryo...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/1769298 |
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| _version_ | 1832545515561025536 |
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| author | Zhong-Yan Chen Fei Chen Nan Cao Zhi-Wen Zhou Huang-Tian Yang |
| author_facet | Zhong-Yan Chen Fei Chen Nan Cao Zhi-Wen Zhou Huang-Tian Yang |
| author_sort | Zhong-Yan Chen |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) play important roles in cell fate decisions. However, the miRNAs and their targets involved in the regulation of cardiac lineage specification are largely unexplored. Here, we report novel functions of miR-142-3p in the regulation of cardiomyocyte differentiation from mouse embryonic stem cells (mESCs). With a miRNA array screen, we identified a number of miRNAs significantly changed during mESC differentiation into the mesodermal and cardiac progenitor cells, and miR-142-3p was one among the markedly downregulated miRNAs. Ectopic expression and inhibition of miR-142-3p did not alter the characteristics of undifferentiated ESCs, whereas ectopic expression of miR-142-3p impaired cardiomyocyte formation. In addition, ectopic expression of miR-142-3p inhibited the expression of a cardiac mesodermal marker gene Mesp1 and downstream cardiac transcription factors Nkx2.5, Tbx5, and Mef2c but not the expression of three germ layer-specific genes. We further demonstrated that miR-142-3p targeted the 3′-untranslated region of Mef2c. These results reveal miR-142-3p as an important regulator of early cardiomyocyte differentiation. Our findings provide new knowledge for further understanding of roles and mechanisms of miRNAs as critical regulators of cardiomyocyte differentiation. |
| format | Article |
| id | doaj-art-77914f4ed9b7468882309cfb6581bee7 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-77914f4ed9b7468882309cfb6581bee72025-02-03T07:25:34ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/17692981769298miR-142-3p Contributes to Early Cardiac Fate Decision of Embryonic Stem CellsZhong-Yan Chen0Fei Chen1Nan Cao2Zhi-Wen Zhou3Huang-Tian Yang4Key Laboratory of Stem Cell Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences, Shanghai Jiao Tong University School of Medicine (SJTUSM) and Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences (CAS), Shanghai, ChinaKey Laboratory of Stem Cell Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences, Shanghai Jiao Tong University School of Medicine (SJTUSM) and Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences (CAS), Shanghai, ChinaKey Laboratory of Stem Cell Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences, Shanghai Jiao Tong University School of Medicine (SJTUSM) and Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences (CAS), Shanghai, ChinaDepartment of Cardiology, Shanghai Xuhui District Central Hospital, Shanghai, ChinaKey Laboratory of Stem Cell Biology and Laboratory of Molecular Cardiology, Institute of Health Sciences, Shanghai Jiao Tong University School of Medicine (SJTUSM) and Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences (CAS), Shanghai, ChinaMicroRNAs (miRNAs) play important roles in cell fate decisions. However, the miRNAs and their targets involved in the regulation of cardiac lineage specification are largely unexplored. Here, we report novel functions of miR-142-3p in the regulation of cardiomyocyte differentiation from mouse embryonic stem cells (mESCs). With a miRNA array screen, we identified a number of miRNAs significantly changed during mESC differentiation into the mesodermal and cardiac progenitor cells, and miR-142-3p was one among the markedly downregulated miRNAs. Ectopic expression and inhibition of miR-142-3p did not alter the characteristics of undifferentiated ESCs, whereas ectopic expression of miR-142-3p impaired cardiomyocyte formation. In addition, ectopic expression of miR-142-3p inhibited the expression of a cardiac mesodermal marker gene Mesp1 and downstream cardiac transcription factors Nkx2.5, Tbx5, and Mef2c but not the expression of three germ layer-specific genes. We further demonstrated that miR-142-3p targeted the 3′-untranslated region of Mef2c. These results reveal miR-142-3p as an important regulator of early cardiomyocyte differentiation. Our findings provide new knowledge for further understanding of roles and mechanisms of miRNAs as critical regulators of cardiomyocyte differentiation.http://dx.doi.org/10.1155/2017/1769298 |
| spellingShingle | Zhong-Yan Chen Fei Chen Nan Cao Zhi-Wen Zhou Huang-Tian Yang miR-142-3p Contributes to Early Cardiac Fate Decision of Embryonic Stem Cells Stem Cells International |
| title | miR-142-3p Contributes to Early Cardiac Fate Decision of Embryonic Stem Cells |
| title_full | miR-142-3p Contributes to Early Cardiac Fate Decision of Embryonic Stem Cells |
| title_fullStr | miR-142-3p Contributes to Early Cardiac Fate Decision of Embryonic Stem Cells |
| title_full_unstemmed | miR-142-3p Contributes to Early Cardiac Fate Decision of Embryonic Stem Cells |
| title_short | miR-142-3p Contributes to Early Cardiac Fate Decision of Embryonic Stem Cells |
| title_sort | mir 142 3p contributes to early cardiac fate decision of embryonic stem cells |
| url | http://dx.doi.org/10.1155/2017/1769298 |
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