Characterization of Key Odorants in Soy Sauce and Their Concentration Changes during Storage

To explore the key odorants and their concentration changes in high-salt liquid-state fermentation soy sauce (HLFSS) during storage, solvent extraction coupled with solvent-assisted flavor evaporation was applied to isolate the volatiles in HLFSS, and the volatiles were analyzed by gas chromatograph...

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Main Authors: Rui Wang, Shiyu Chang, Miao Liang, Yajian Wu, Runhu Xin, Yuping Liu, Liang Chen, Shangting Zhou
Format: Article
Language:English
Published: Wiley 2023-01-01
Series:Journal of Food Quality
Online Access:http://dx.doi.org/10.1155/2023/6673089
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author Rui Wang
Shiyu Chang
Miao Liang
Yajian Wu
Runhu Xin
Yuping Liu
Liang Chen
Shangting Zhou
author_facet Rui Wang
Shiyu Chang
Miao Liang
Yajian Wu
Runhu Xin
Yuping Liu
Liang Chen
Shangting Zhou
author_sort Rui Wang
collection DOAJ
description To explore the key odorants and their concentration changes in high-salt liquid-state fermentation soy sauce (HLFSS) during storage, solvent extraction coupled with solvent-assisted flavor evaporation was applied to isolate the volatiles in HLFSS, and the volatiles were analyzed by gas chromatograph-mass spectrometry-olfactory and gas chromatograph-flame ionization detector-olfactometry combined with the aroma extract dilution assay. A total of 37 odor-active compounds with flavor dilution (FD) factors ranging from 1 to 1024 were identified, and 23 aroma components with odor activity values ≥1 were considered as key odorants. Methional, 3-methylbutanoic acid, phenethyl alcohol, sotolon, 4-hydroxy-2-methyl-5-ethyl-3(2H)-furanone (HEMF), and 4-hydroxy-2,5-dimethylfuran-3(2H)-one (HDMF) had the highest FD factors of 1024. The results of quantitative analysis through the internal standard curve showed that the concentrations of HEMF, HDMF, acetic acid, 1-hydroxy-2-butanone, acetol, and furfuryl alcohol varied greatly during storage, so these compounds might be used as the indicators to determine the shelf life of HLFSS. The recombination experiment also confirmed the important contribution of these compounds. This result will provide valuable information for understanding the flavor changes of HLFSS.
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institution Kabale University
issn 1745-4557
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publishDate 2023-01-01
publisher Wiley
record_format Article
series Journal of Food Quality
spelling doaj-art-77911ae0534246d6a3fdd079543fb1502025-02-03T06:45:14ZengWileyJournal of Food Quality1745-45572023-01-01202310.1155/2023/6673089Characterization of Key Odorants in Soy Sauce and Their Concentration Changes during StorageRui Wang0Shiyu Chang1Miao Liang2Yajian Wu3Runhu Xin4Yuping Liu5Liang Chen6Shangting Zhou7School of Light IndustrySchool of Light IndustrySchool of Light IndustrySchool of Light IndustrySchool of Light IndustrySchool of Light IndustryJiajia Food Group Co. Ltd.Jiajia Food Group Co. Ltd.To explore the key odorants and their concentration changes in high-salt liquid-state fermentation soy sauce (HLFSS) during storage, solvent extraction coupled with solvent-assisted flavor evaporation was applied to isolate the volatiles in HLFSS, and the volatiles were analyzed by gas chromatograph-mass spectrometry-olfactory and gas chromatograph-flame ionization detector-olfactometry combined with the aroma extract dilution assay. A total of 37 odor-active compounds with flavor dilution (FD) factors ranging from 1 to 1024 were identified, and 23 aroma components with odor activity values ≥1 were considered as key odorants. Methional, 3-methylbutanoic acid, phenethyl alcohol, sotolon, 4-hydroxy-2-methyl-5-ethyl-3(2H)-furanone (HEMF), and 4-hydroxy-2,5-dimethylfuran-3(2H)-one (HDMF) had the highest FD factors of 1024. The results of quantitative analysis through the internal standard curve showed that the concentrations of HEMF, HDMF, acetic acid, 1-hydroxy-2-butanone, acetol, and furfuryl alcohol varied greatly during storage, so these compounds might be used as the indicators to determine the shelf life of HLFSS. The recombination experiment also confirmed the important contribution of these compounds. This result will provide valuable information for understanding the flavor changes of HLFSS.http://dx.doi.org/10.1155/2023/6673089
spellingShingle Rui Wang
Shiyu Chang
Miao Liang
Yajian Wu
Runhu Xin
Yuping Liu
Liang Chen
Shangting Zhou
Characterization of Key Odorants in Soy Sauce and Their Concentration Changes during Storage
Journal of Food Quality
title Characterization of Key Odorants in Soy Sauce and Their Concentration Changes during Storage
title_full Characterization of Key Odorants in Soy Sauce and Their Concentration Changes during Storage
title_fullStr Characterization of Key Odorants in Soy Sauce and Their Concentration Changes during Storage
title_full_unstemmed Characterization of Key Odorants in Soy Sauce and Their Concentration Changes during Storage
title_short Characterization of Key Odorants in Soy Sauce and Their Concentration Changes during Storage
title_sort characterization of key odorants in soy sauce and their concentration changes during storage
url http://dx.doi.org/10.1155/2023/6673089
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