Effects of temperature on the growth, development and extracellular enzyme activities of Pleurotus pulmonarius
In order to research the formation mechanism of Pleurotus pulmonarius fruit body with essential low temperature treatment, the mycelial growth at different temperatures were studied. The mycelia were cultivated at 20, 22, 24, 26, 28, 30 ℃, respectively, and then they were cultivated at 4, 8, 12 ℃ an...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2020-04-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2019.05.211 |
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| Summary: | In order to research the formation mechanism of Pleurotus pulmonarius fruit body with essential low temperature treatment, the mycelial growth at different temperatures were studied. The mycelia were cultivated at 20, 22, 24, 26, 28, 30 ℃, respectively, and then they were cultivated at 4, 8, 12 ℃ and 25 ℃ (CK) for 3-24 h after the mycelia fully lasted 30 d, and the activities of five extracellular enzymes, including carboxymethyl cellulase, filter paper cellulase, hemicellulase, amylase and laccase were tested. At the same time, the economical characters of P. pulmonarius were analyzed at different cultivation temperatures (8, 12, 16, 20 ℃） and time (6, 9, 12, 15, 18 h) in summer. The results showed that P. pulmonarius grew fast and had the strongest mycelium growth at 26 ℃. The mycelial growth became weaker with the increase of cultivation temperature. At the same low temperature, the activities of the five extracellular enzymes first increased, peaked at 9-18 h, and then decreased on the whole. At the same time, the activities of carboxymethyl cellulase, filter paper cellulase, hemicellulase and amylase were stronger at 4, 8 and 12 ℃ than at 25 ℃ (CK), but the laccase activity was lower than CK, and the lower cultivation temperature, the lower laccase activity. The cultivation experiment showed that the average yield of one fungus bag exceeded 100 g at 8 ℃ for 9-15 h or 16 ℃ for 12 h. The speed of mushroom bud formation and fruit body uniformity were excellent at 16 ℃ for 12 h. In conclusion, the activities of five extracellular enzymes are regulated by low temperature treatment, and then the degradation of macromolecule nutrients in the matrix and nutrient absorption of mycelia are adjusted. The above results are of great significance for the further study on the formation mechanism of P. pulmonarius fruit body and the guidance of the production in summer. |
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| ISSN: | 1008-9209 2097-5155 |