Comprehensive Analysis and Production of Powdered Starter Using <i>Saccharomyces cerevisiae</i> YM45

In order to secure domestic microbial resources, a fruit-flavored yeast was selected, and a starter manufacturing technology was developed to evaluate its quality characteristics. Among 26 yeast strains analyzed using an electronic nose, <i>Saccharomyces cerevisiae</i> YM45 isolated from...

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Main Authors: Su Jeong Lee, Joo-Yeon Kim, So-Young Kim, Soo-Hwan Yeo, Chan-Woo Kim
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Fermentation
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Online Access:https://www.mdpi.com/2311-5637/11/4/203
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author Su Jeong Lee
Joo-Yeon Kim
So-Young Kim
Soo-Hwan Yeo
Chan-Woo Kim
author_facet Su Jeong Lee
Joo-Yeon Kim
So-Young Kim
Soo-Hwan Yeo
Chan-Woo Kim
author_sort Su Jeong Lee
collection DOAJ
description In order to secure domestic microbial resources, a fruit-flavored yeast was selected, and a starter manufacturing technology was developed to evaluate its quality characteristics. Among 26 yeast strains analyzed using an electronic nose, <i>Saccharomyces cerevisiae</i> YM45 isolated from <i>Makgeolli</i> was identified as having high levels of methyl formate and propan-2-one, compounds that are associated with fruity and sweet aroma. A powdered starter was then produced with <i>S. cerevisiae</i> YM45, and its quality characteristics were analyzed. When cyclodextrin was used as an excipient, with water content at 4.7%, bacterial contamination was found to be 2.30 log CFU/g, which ensures quality and safety. The rehydration rate, assessed using a PBS buffer, showed a high survival rate of 8.7 log CFU/g, which is a suitable condition for preserving yeast activity. These findings suggest that domestically sourced microbial resources can serve as viable alternatives to imported strains, with potential applications in industrial fermentation.
format Article
id doaj-art-777073cfe5d14dada71aa6e2e662d0eb
institution DOAJ
issn 2311-5637
language English
publishDate 2025-04-01
publisher MDPI AG
record_format Article
series Fermentation
spelling doaj-art-777073cfe5d14dada71aa6e2e662d0eb2025-08-20T03:13:30ZengMDPI AGFermentation2311-56372025-04-0111420310.3390/fermentation11040203Comprehensive Analysis and Production of Powdered Starter Using <i>Saccharomyces cerevisiae</i> YM45Su Jeong Lee0Joo-Yeon Kim1So-Young Kim2Soo-Hwan Yeo3Chan-Woo Kim4Fermented and Processed Food Research Division, Department of Food Sciences, NICS, RDA, Wanju 55365, Republic of KoreaFermented and Processed Food Research Division, Department of Food Sciences, NICS, RDA, Wanju 55365, Republic of KoreaFermented and Processed Food Research Division, Department of Food Sciences, NICS, RDA, Wanju 55365, Republic of KoreaSulseam, Agricultural Corporation, Yongin 17158, Republic of KoreaFermented and Processed Food Research Division, Department of Food Sciences, NICS, RDA, Wanju 55365, Republic of KoreaIn order to secure domestic microbial resources, a fruit-flavored yeast was selected, and a starter manufacturing technology was developed to evaluate its quality characteristics. Among 26 yeast strains analyzed using an electronic nose, <i>Saccharomyces cerevisiae</i> YM45 isolated from <i>Makgeolli</i> was identified as having high levels of methyl formate and propan-2-one, compounds that are associated with fruity and sweet aroma. A powdered starter was then produced with <i>S. cerevisiae</i> YM45, and its quality characteristics were analyzed. When cyclodextrin was used as an excipient, with water content at 4.7%, bacterial contamination was found to be 2.30 log CFU/g, which ensures quality and safety. The rehydration rate, assessed using a PBS buffer, showed a high survival rate of 8.7 log CFU/g, which is a suitable condition for preserving yeast activity. These findings suggest that domestically sourced microbial resources can serve as viable alternatives to imported strains, with potential applications in industrial fermentation.https://www.mdpi.com/2311-5637/11/4/203yeast<i>Saccharomyces cerevisiae</i>starterfermentation
spellingShingle Su Jeong Lee
Joo-Yeon Kim
So-Young Kim
Soo-Hwan Yeo
Chan-Woo Kim
Comprehensive Analysis and Production of Powdered Starter Using <i>Saccharomyces cerevisiae</i> YM45
Fermentation
yeast
<i>Saccharomyces cerevisiae</i>
starter
fermentation
title Comprehensive Analysis and Production of Powdered Starter Using <i>Saccharomyces cerevisiae</i> YM45
title_full Comprehensive Analysis and Production of Powdered Starter Using <i>Saccharomyces cerevisiae</i> YM45
title_fullStr Comprehensive Analysis and Production of Powdered Starter Using <i>Saccharomyces cerevisiae</i> YM45
title_full_unstemmed Comprehensive Analysis and Production of Powdered Starter Using <i>Saccharomyces cerevisiae</i> YM45
title_short Comprehensive Analysis and Production of Powdered Starter Using <i>Saccharomyces cerevisiae</i> YM45
title_sort comprehensive analysis and production of powdered starter using i saccharomyces cerevisiae i ym45
topic yeast
<i>Saccharomyces cerevisiae</i>
starter
fermentation
url https://www.mdpi.com/2311-5637/11/4/203
work_keys_str_mv AT sujeonglee comprehensiveanalysisandproductionofpowderedstarterusingisaccharomycescerevisiaeiym45
AT jooyeonkim comprehensiveanalysisandproductionofpowderedstarterusingisaccharomycescerevisiaeiym45
AT soyoungkim comprehensiveanalysisandproductionofpowderedstarterusingisaccharomycescerevisiaeiym45
AT soohwanyeo comprehensiveanalysisandproductionofpowderedstarterusingisaccharomycescerevisiaeiym45
AT chanwookim comprehensiveanalysisandproductionofpowderedstarterusingisaccharomycescerevisiaeiym45