LIMD1-AS1 promotes the progression of prostate cancer and affects the function of prostate cancer cells by down-regulating miR-29c-3p
Abstract Purpose Prostate cancer (PCa) is a prevalent and lethal malignancy affecting males, with a considerable proportion of patients experiencing poor survival outcomes. The regulatory role of LIMD1-AS1 in the initiation and progression of PCa is emerging as a significant factor, however, the pre...
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| Format: | Article |
| Language: | English |
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Springer
2024-12-01
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| Series: | Journal of Cancer Research and Clinical Oncology |
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| Online Access: | https://doi.org/10.1007/s00432-024-06046-0 |
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| _version_ | 1823863278496907264 |
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| author | Yongsheng Yu Nan He Zhaolu Song Chang Wang Jinhuang Xu |
| author_facet | Yongsheng Yu Nan He Zhaolu Song Chang Wang Jinhuang Xu |
| author_sort | Yongsheng Yu |
| collection | DOAJ |
| description | Abstract Purpose Prostate cancer (PCa) is a prevalent and lethal malignancy affecting males, with a considerable proportion of patients experiencing poor survival outcomes. The regulatory role of LIMD1-AS1 in the initiation and progression of PCa is emerging as a significant factor, however, the precise mechanisms governing its influence are yet to be fully elucidated. Methods qRT-PCR was employed to assess the expression of LIMD1-AS1 and miR-29c-3p. The Cell Counting Kit-8 (CCK-8) was used to assess cell proliferation in PCa cells. Apoptosis rates were determined using flow cytometry. Cell migration and invasion were evaluated using the transwell assay. The targeted relationship of LIMD1-AS1 and miR-29c-3p was confirmed through dual-luciferase reporter gene analysis. Results Increased expression of LIMD1-AS1 and decreased expression of miR-29c-3p were observed in both tumor tissues and serum from PCa patients. LIMD1-AS1 exhibited diagnostic and prognostic significance in PCa patients. Functionally, LIMD1-AS1 modulated the expression of miR-29c-3p to potentiate the proliferative, migratory, and invasive capabilities of PCa cells while concurrently inhibiting apoptosis. Conclusion LncRNA LIMD1-AS1 promotes the advancement of PCa by regulating miR-29c-3p, indicating that LIMD1-AS1/miR-29c-3p axis could serve as potential therapeutic targets for the therapeutic intervention of PCa. |
| format | Article |
| id | doaj-art-775caf38e779441ba2bf932ceed3bcc5 |
| institution | Kabale University |
| issn | 1432-1335 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Cancer Research and Clinical Oncology |
| spelling | doaj-art-775caf38e779441ba2bf932ceed3bcc52025-02-09T12:10:26ZengSpringerJournal of Cancer Research and Clinical Oncology1432-13352024-12-01151111010.1007/s00432-024-06046-0LIMD1-AS1 promotes the progression of prostate cancer and affects the function of prostate cancer cells by down-regulating miR-29c-3pYongsheng Yu0Nan He1Zhaolu Song2Chang Wang3Jinhuang Xu4Department of Urinary Surgery, The Fourth Affiliated Hospital of Guangzhou Medical UniversityDepartment of Pharmacology, Wuhan University TaiKang Medical School (School of Basic Medical Sciences)Department of Urology Surgery, Jiaozhou Central Hospital of QingdaoOrgan Transplant Center, The First Affiliated Hospital, Sun Yat-sen UniversityDepartment of Trauma and Joint Surgery, The Fourth Affiliated Hospital of Guangzhou Medical UniversityAbstract Purpose Prostate cancer (PCa) is a prevalent and lethal malignancy affecting males, with a considerable proportion of patients experiencing poor survival outcomes. The regulatory role of LIMD1-AS1 in the initiation and progression of PCa is emerging as a significant factor, however, the precise mechanisms governing its influence are yet to be fully elucidated. Methods qRT-PCR was employed to assess the expression of LIMD1-AS1 and miR-29c-3p. The Cell Counting Kit-8 (CCK-8) was used to assess cell proliferation in PCa cells. Apoptosis rates were determined using flow cytometry. Cell migration and invasion were evaluated using the transwell assay. The targeted relationship of LIMD1-AS1 and miR-29c-3p was confirmed through dual-luciferase reporter gene analysis. Results Increased expression of LIMD1-AS1 and decreased expression of miR-29c-3p were observed in both tumor tissues and serum from PCa patients. LIMD1-AS1 exhibited diagnostic and prognostic significance in PCa patients. Functionally, LIMD1-AS1 modulated the expression of miR-29c-3p to potentiate the proliferative, migratory, and invasive capabilities of PCa cells while concurrently inhibiting apoptosis. Conclusion LncRNA LIMD1-AS1 promotes the advancement of PCa by regulating miR-29c-3p, indicating that LIMD1-AS1/miR-29c-3p axis could serve as potential therapeutic targets for the therapeutic intervention of PCa.https://doi.org/10.1007/s00432-024-06046-0Prostate cancerLIMD1-AS1miR-29c-3pDiagnosisPrognosis |
| spellingShingle | Yongsheng Yu Nan He Zhaolu Song Chang Wang Jinhuang Xu LIMD1-AS1 promotes the progression of prostate cancer and affects the function of prostate cancer cells by down-regulating miR-29c-3p Journal of Cancer Research and Clinical Oncology Prostate cancer LIMD1-AS1 miR-29c-3p Diagnosis Prognosis |
| title | LIMD1-AS1 promotes the progression of prostate cancer and affects the function of prostate cancer cells by down-regulating miR-29c-3p |
| title_full | LIMD1-AS1 promotes the progression of prostate cancer and affects the function of prostate cancer cells by down-regulating miR-29c-3p |
| title_fullStr | LIMD1-AS1 promotes the progression of prostate cancer and affects the function of prostate cancer cells by down-regulating miR-29c-3p |
| title_full_unstemmed | LIMD1-AS1 promotes the progression of prostate cancer and affects the function of prostate cancer cells by down-regulating miR-29c-3p |
| title_short | LIMD1-AS1 promotes the progression of prostate cancer and affects the function of prostate cancer cells by down-regulating miR-29c-3p |
| title_sort | limd1 as1 promotes the progression of prostate cancer and affects the function of prostate cancer cells by down regulating mir 29c 3p |
| topic | Prostate cancer LIMD1-AS1 miR-29c-3p Diagnosis Prognosis |
| url | https://doi.org/10.1007/s00432-024-06046-0 |
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