Feed additives for methane mitigation: Recommendations for identification and selection of bioactive compounds to develop antimethanogenic feed additives
ABSTRACT: Despite the increasing interest in developing antimethanogenic additives to reduce enteric methane (CH4) emissions and the extensive research conducted over the last decades, the global livestock industry has a very limited number of antimethanogenic feed additives (AMFA) available that ca...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Journal of Dairy Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030224014000 |
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| author | Zoey Durmic Evert C. Duin André Bannink Alejandro Belanche Vincenzo Carbone M. Dolores Carro Max Crüsemann Veerle Fievez Florencia Garcia Alex Hristov Miroslav Joch Gonzalo Martinez-Fernandez Stefan Muetzel Emilio M. Ungerfeld Min Wang David R. Yáñez-Ruiz |
| author_facet | Zoey Durmic Evert C. Duin André Bannink Alejandro Belanche Vincenzo Carbone M. Dolores Carro Max Crüsemann Veerle Fievez Florencia Garcia Alex Hristov Miroslav Joch Gonzalo Martinez-Fernandez Stefan Muetzel Emilio M. Ungerfeld Min Wang David R. Yáñez-Ruiz |
| author_sort | Zoey Durmic |
| collection | DOAJ |
| description | ABSTRACT: Despite the increasing interest in developing antimethanogenic additives to reduce enteric methane (CH4) emissions and the extensive research conducted over the last decades, the global livestock industry has a very limited number of antimethanogenic feed additives (AMFA) available that can deliver substantial reduction, and they have generally not reached the market yet. This work provides technical recommendations and guidelines for conducting tests intended to screen the potential to reduce, directly or indirectly, enteric CH4 of compounds before they can be further assessed in in vivo conditions. The steps involved in this work cover the discovery, isolation, and identification of compounds capable of affecting CH4 production by rumen microbes, followed by in vitro laboratory testing of potential candidates. The finding of new bioactive compounds as AMFA can be based on 2 approaches: empirical and mechanistic. The empirical approach involves obtaining and screening compounds present in databases and repositories that potentially possess the desired effect but have not yet been tested, screening natural sources of secondary compounds such as plants, fungi, and algae for their antimethanogenic effects, or examining compounds with antimethanogenic effect on microbes in other research domains outside the rumen. In contrast, the mechanistic approach is the theoretical process of discovery new bioactive compounds based on existing knowledge of a biological target or process. The in vitro methodologies reviewed include examining effects at the subcellular level, in single pure cultures of methanogens and examining in more complex mixed rumen microbial populations. Simple in vitro methodologies (subcellular assessments and batch culture) allow testing a large number of compounds, whereas more complex systems simulating the rumen microbial ecosystem can test a limited number of candidates but provide better insight about the antimethanogenic efficacy. This work collated the main advantages, limitations, and technical recommendations associated with each step and methodology use during the identification and screening of AMFA candidates. |
| format | Article |
| id | doaj-art-7755a4f69d054e94a4af844a4fadb1cd |
| institution | OA Journals |
| issn | 0022-0302 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj-art-7755a4f69d054e94a4af844a4fadb1cd2025-08-20T02:39:08ZengElsevierJournal of Dairy Science0022-03022025-01-01108130232110.3168/jds.2024-25045Feed additives for methane mitigation: Recommendations for identification and selection of bioactive compounds to develop antimethanogenic feed additivesZoey Durmic0Evert C. Duin1André Bannink2Alejandro Belanche3Vincenzo Carbone4M. Dolores Carro5Max Crüsemann6Veerle Fievez7Florencia Garcia8Alex Hristov9Miroslav Joch10Gonzalo Martinez-Fernandez11Stefan Muetzel12Emilio M. Ungerfeld13Min Wang14David R. Yáñez-Ruiz15School of Agriculture and Environment, The University of Western Australia, Crawley, WA, 6009, AustraliaDepartment of Chemistry and Biochemistry, Auburn University, Auburn, AL 36849Wageningen Livestock Research, Wageningen University & Research, 6700 AH Wageningen, the NetherlandsDepartamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, 50013, Zaragoza, SpainAgResearch Ltd. Grasslands, Palmerston North, 4442, New ZealandDepartamento de Producción Agraria, Universidad Politécnica de Madrid, ETSIAAB, 28040 Madrid, SpainInstitute of Pharmaceutical Biology, University of Bonn, 53115 Bonn, GermanyDepartment of Animal Sciences and Aquatic Ecology, Ghent University, 9000 Gent, BelgiumUniversidad Nacional de Córdoba, Facultad de Ciencias Agropecuarias, Córdoba, 5000, Argentina; Corresponding authorsDepartment of Animal Science, The Pennsylvania State University, University Park, PA 16802Department of Nutrition and Feeding of Farm Animals, Institute of Animal Science, 104 00 Prague, Czech RepublicCSIRO, Agriculture and Food, St Lucia, QLD, Australia, 4067AgResearch Ltd. Grasslands, Palmerston North, 4442, New ZealandInstituto de Investigaciones Agropecuarias, Centro Regional de Investigación Carillanca, Vilcún, La Araucanía, Chile, 4880000Institute of Subtropical Agriculture, Chinese Academy of Sciences, 410125, Changsha, Hunan, ChinaEstación Experimental del Zaidín, CSIC, 18008 Granada, Spain; Corresponding authorsABSTRACT: Despite the increasing interest in developing antimethanogenic additives to reduce enteric methane (CH4) emissions and the extensive research conducted over the last decades, the global livestock industry has a very limited number of antimethanogenic feed additives (AMFA) available that can deliver substantial reduction, and they have generally not reached the market yet. This work provides technical recommendations and guidelines for conducting tests intended to screen the potential to reduce, directly or indirectly, enteric CH4 of compounds before they can be further assessed in in vivo conditions. The steps involved in this work cover the discovery, isolation, and identification of compounds capable of affecting CH4 production by rumen microbes, followed by in vitro laboratory testing of potential candidates. The finding of new bioactive compounds as AMFA can be based on 2 approaches: empirical and mechanistic. The empirical approach involves obtaining and screening compounds present in databases and repositories that potentially possess the desired effect but have not yet been tested, screening natural sources of secondary compounds such as plants, fungi, and algae for their antimethanogenic effects, or examining compounds with antimethanogenic effect on microbes in other research domains outside the rumen. In contrast, the mechanistic approach is the theoretical process of discovery new bioactive compounds based on existing knowledge of a biological target or process. The in vitro methodologies reviewed include examining effects at the subcellular level, in single pure cultures of methanogens and examining in more complex mixed rumen microbial populations. Simple in vitro methodologies (subcellular assessments and batch culture) allow testing a large number of compounds, whereas more complex systems simulating the rumen microbial ecosystem can test a limited number of candidates but provide better insight about the antimethanogenic efficacy. This work collated the main advantages, limitations, and technical recommendations associated with each step and methodology use during the identification and screening of AMFA candidates.http://www.sciencedirect.com/science/article/pii/S0022030224014000in silicodockingin vitromethanerumen |
| spellingShingle | Zoey Durmic Evert C. Duin André Bannink Alejandro Belanche Vincenzo Carbone M. Dolores Carro Max Crüsemann Veerle Fievez Florencia Garcia Alex Hristov Miroslav Joch Gonzalo Martinez-Fernandez Stefan Muetzel Emilio M. Ungerfeld Min Wang David R. Yáñez-Ruiz Feed additives for methane mitigation: Recommendations for identification and selection of bioactive compounds to develop antimethanogenic feed additives Journal of Dairy Science in silico docking in vitro methane rumen |
| title | Feed additives for methane mitigation: Recommendations for identification and selection of bioactive compounds to develop antimethanogenic feed additives |
| title_full | Feed additives for methane mitigation: Recommendations for identification and selection of bioactive compounds to develop antimethanogenic feed additives |
| title_fullStr | Feed additives for methane mitigation: Recommendations for identification and selection of bioactive compounds to develop antimethanogenic feed additives |
| title_full_unstemmed | Feed additives for methane mitigation: Recommendations for identification and selection of bioactive compounds to develop antimethanogenic feed additives |
| title_short | Feed additives for methane mitigation: Recommendations for identification and selection of bioactive compounds to develop antimethanogenic feed additives |
| title_sort | feed additives for methane mitigation recommendations for identification and selection of bioactive compounds to develop antimethanogenic feed additives |
| topic | in silico docking in vitro methane rumen |
| url | http://www.sciencedirect.com/science/article/pii/S0022030224014000 |
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