Protective effects of tilapia (Oreochromis niloticus) skin gelatin hydrolysates on osteoporosis rats induced by retinoic acid
Tilapia skin gelatin hydrolysates (TSGH) were obtained by complex protease hydrolysis. The amino acid sequences of 50 peptides in TSGH were identified, and most of these peptides were found to contain the -Gly-Pro-sequence. The osteoporosis (OP) rat model induced by retinoic acid was prepared, and t...
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Tsinghua University Press
2022-11-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022000908 |
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| author | Bingtong Liu Liping Sun Yongliang Zhuang |
| author_facet | Bingtong Liu Liping Sun Yongliang Zhuang |
| author_sort | Bingtong Liu |
| collection | DOAJ |
| description | Tilapia skin gelatin hydrolysates (TSGH) were obtained by complex protease hydrolysis. The amino acid sequences of 50 peptides in TSGH were identified, and most of these peptides were found to contain the -Gly-Pro-sequence. The osteoporosis (OP) rat model induced by retinoic acid was prepared, and the effects of different doses of TSGH on OP in vivo were evaluated. Serum calcium (Ca) and phosphate (P), alkaline phosphatase activity, and osteocalcin levels in OP rats were regulated by TSGH. The bone length, dry weight index, maximum load, and Ca content of OP rats significantly increased by treatment TSGH in a dose-dependent manner. Micro-CT images of the femurs and tibias of the rats indicated that the bone mineral density, cortical bone thickness, and cortical/trabecular bone area ratios were recovered and that OP symptoms were improved. Tartrate-resistant acid phosphate and hematoxylin-eosin staining showed that osteoclast numbers and histomorphological changes in the femurs in OP rats could be recovered by TSGH. |
| format | Article |
| id | doaj-art-7754e83a5ce447629314faf8e989f8aa |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-7754e83a5ce447629314faf8e989f8aa2025-02-02T23:24:56ZengTsinghua University PressFood Science and Human Wellness2213-45302022-11-0111615001507Protective effects of tilapia (Oreochromis niloticus) skin gelatin hydrolysates on osteoporosis rats induced by retinoic acidBingtong Liu0Liping Sun1Yongliang Zhuang2Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, ChinaFaculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, ChinaCorresponding author: Fax: +86 871 65920216.; Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, ChinaTilapia skin gelatin hydrolysates (TSGH) were obtained by complex protease hydrolysis. The amino acid sequences of 50 peptides in TSGH were identified, and most of these peptides were found to contain the -Gly-Pro-sequence. The osteoporosis (OP) rat model induced by retinoic acid was prepared, and the effects of different doses of TSGH on OP in vivo were evaluated. Serum calcium (Ca) and phosphate (P), alkaline phosphatase activity, and osteocalcin levels in OP rats were regulated by TSGH. The bone length, dry weight index, maximum load, and Ca content of OP rats significantly increased by treatment TSGH in a dose-dependent manner. Micro-CT images of the femurs and tibias of the rats indicated that the bone mineral density, cortical bone thickness, and cortical/trabecular bone area ratios were recovered and that OP symptoms were improved. Tartrate-resistant acid phosphate and hematoxylin-eosin staining showed that osteoclast numbers and histomorphological changes in the femurs in OP rats could be recovered by TSGH.http://www.sciencedirect.com/science/article/pii/S2213453022000908Amino acid sequenceBone growthMicro-CTOsteoporosisRetinoic acidTilapia skin gelatin hydrolysates |
| spellingShingle | Bingtong Liu Liping Sun Yongliang Zhuang Protective effects of tilapia (Oreochromis niloticus) skin gelatin hydrolysates on osteoporosis rats induced by retinoic acid Food Science and Human Wellness Amino acid sequence Bone growth Micro-CT Osteoporosis Retinoic acid Tilapia skin gelatin hydrolysates |
| title | Protective effects of tilapia (Oreochromis niloticus) skin gelatin hydrolysates on osteoporosis rats induced by retinoic acid |
| title_full | Protective effects of tilapia (Oreochromis niloticus) skin gelatin hydrolysates on osteoporosis rats induced by retinoic acid |
| title_fullStr | Protective effects of tilapia (Oreochromis niloticus) skin gelatin hydrolysates on osteoporosis rats induced by retinoic acid |
| title_full_unstemmed | Protective effects of tilapia (Oreochromis niloticus) skin gelatin hydrolysates on osteoporosis rats induced by retinoic acid |
| title_short | Protective effects of tilapia (Oreochromis niloticus) skin gelatin hydrolysates on osteoporosis rats induced by retinoic acid |
| title_sort | protective effects of tilapia oreochromis niloticus skin gelatin hydrolysates on osteoporosis rats induced by retinoic acid |
| topic | Amino acid sequence Bone growth Micro-CT Osteoporosis Retinoic acid Tilapia skin gelatin hydrolysates |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022000908 |
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