Evolution of strain diversity and virulence factor repertoire in pediatric Staphylococcus aureus isolates.
<h4>Background</h4>Invasive Staphylococcus aureus infections cause high morbidity and mortality in children and adults. With rising antimicrobial resistance, optimal prevention strategies and novel therapeutics are needed. As an effective vaccine remains elusive, characterization of inva...
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| Format: | Article |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0326353 |
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| author | Margaret Free Nicole Soper James C Slaughter Andries Feder Colleen Bianco Ahmed M Moustafa Paul Planet C Buddy Creech Isaac Thomsen |
| author_facet | Margaret Free Nicole Soper James C Slaughter Andries Feder Colleen Bianco Ahmed M Moustafa Paul Planet C Buddy Creech Isaac Thomsen |
| author_sort | Margaret Free |
| collection | DOAJ |
| description | <h4>Background</h4>Invasive Staphylococcus aureus infections cause high morbidity and mortality in children and adults. With rising antimicrobial resistance, optimal prevention strategies and novel therapeutics are needed. As an effective vaccine remains elusive, characterization of invasive isolates over time is required to identify determinants of invasive infection.<h4>Methods</h4>S. aureus isolates recovered from children with invasive infection and those with colonization were obtained. Isolates were examined by whole genome sequencing to evaluate gene repertoire, sequence type, clonal complex, and phylogenetic characterization, and isolate characteristics were correlated to clinical data.<h4>Results</h4>118 children with invasive S. aureus infections were enrolled; 56% of infections were caused by methicillin-susceptible S. aureus (MSSA). Methicillin-resistance (MRSA) was associated with increased inflammation, though clinical outcomes of MRSA vs MSSA did not differ. Colonization isolates exhibited higher sequence type diversity than invasive isolates. Nine distinct clonal complexes (CC) were identified among all isolates; CC8 and CC5 were associated with higher clinical severity scores. Accessory gene regulator locus type 1, Panton-Valentine Leukocidin, and arginine catabolic mobile element declined over time. Staphylokinase and leukocidin ED were associated with invasive infection, while enterotoxin B was more frequent in colonizing isolates.<h4>Conclusions</h4>We observed a significant expansion in sequence type diversity among invasive clinical isolates over 12 years with the emergence of newly invasive clones in recent years. The presence of staphylokinase and LukED were associated with invasive infection over time. These findings provide insights into the pathogenesis of invasive S. aureus and may provide putative targets for immunologic approaches to prevention. |
| format | Article |
| id | doaj-art-774fa94393ba41d98ce141afb7fb64a5 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-774fa94393ba41d98ce141afb7fb64a52025-08-20T03:23:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01207e032635310.1371/journal.pone.0326353Evolution of strain diversity and virulence factor repertoire in pediatric Staphylococcus aureus isolates.Margaret FreeNicole SoperJames C SlaughterAndries FederColleen BiancoAhmed M MoustafaPaul PlanetC Buddy CreechIsaac Thomsen<h4>Background</h4>Invasive Staphylococcus aureus infections cause high morbidity and mortality in children and adults. With rising antimicrobial resistance, optimal prevention strategies and novel therapeutics are needed. As an effective vaccine remains elusive, characterization of invasive isolates over time is required to identify determinants of invasive infection.<h4>Methods</h4>S. aureus isolates recovered from children with invasive infection and those with colonization were obtained. Isolates were examined by whole genome sequencing to evaluate gene repertoire, sequence type, clonal complex, and phylogenetic characterization, and isolate characteristics were correlated to clinical data.<h4>Results</h4>118 children with invasive S. aureus infections were enrolled; 56% of infections were caused by methicillin-susceptible S. aureus (MSSA). Methicillin-resistance (MRSA) was associated with increased inflammation, though clinical outcomes of MRSA vs MSSA did not differ. Colonization isolates exhibited higher sequence type diversity than invasive isolates. Nine distinct clonal complexes (CC) were identified among all isolates; CC8 and CC5 were associated with higher clinical severity scores. Accessory gene regulator locus type 1, Panton-Valentine Leukocidin, and arginine catabolic mobile element declined over time. Staphylokinase and leukocidin ED were associated with invasive infection, while enterotoxin B was more frequent in colonizing isolates.<h4>Conclusions</h4>We observed a significant expansion in sequence type diversity among invasive clinical isolates over 12 years with the emergence of newly invasive clones in recent years. The presence of staphylokinase and LukED were associated with invasive infection over time. These findings provide insights into the pathogenesis of invasive S. aureus and may provide putative targets for immunologic approaches to prevention.https://doi.org/10.1371/journal.pone.0326353 |
| spellingShingle | Margaret Free Nicole Soper James C Slaughter Andries Feder Colleen Bianco Ahmed M Moustafa Paul Planet C Buddy Creech Isaac Thomsen Evolution of strain diversity and virulence factor repertoire in pediatric Staphylococcus aureus isolates. PLoS ONE |
| title | Evolution of strain diversity and virulence factor repertoire in pediatric Staphylococcus aureus isolates. |
| title_full | Evolution of strain diversity and virulence factor repertoire in pediatric Staphylococcus aureus isolates. |
| title_fullStr | Evolution of strain diversity and virulence factor repertoire in pediatric Staphylococcus aureus isolates. |
| title_full_unstemmed | Evolution of strain diversity and virulence factor repertoire in pediatric Staphylococcus aureus isolates. |
| title_short | Evolution of strain diversity and virulence factor repertoire in pediatric Staphylococcus aureus isolates. |
| title_sort | evolution of strain diversity and virulence factor repertoire in pediatric staphylococcus aureus isolates |
| url | https://doi.org/10.1371/journal.pone.0326353 |
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