Innovative nucleic acid detection of Clostridioides difficile utilizing the PAM-unconventional, one-step LAMP/CRISPR-Cas12b detection platforms

Innovative nucleic acid detection of Clostridioides difficile utilizing the PAM-unconventional, one-step LAMP/CRISPR-Cas12b detection platforms

IntroductionClostridioides difficile (C. difficile), a human pathogen that causes diarrhea and colon lesions, has garnered widespread attention. Rapid and accurate detection of bacterial virulence factors is essential for the diagnosis of C. difficile infection (CDI). To date, numerous laboratory te...

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Bibliographic Details
Main Authors: Yizhuo Zhang, Luqin Lv, Su Xu, Yijian Chen
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-05-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
Clostridioides difficile
LAMP
CRISPR-Cas12b
nucleic acid detection
point-of-care testing
Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2025.1594271/full
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