Automation of biochemical assays using an open-sourced, inexpensive robotic liquid handler
High Throughput Screening is crucial in pharmaceutical companies for efficient testing in drug discovery and development. Our Vaccines Analytical Research and Development (V-AR&D) department extensively uses robotic liquid handlers in their High Throughput Analytics (HTA) group for assay develop...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | SLAS Technology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2472630324000876 |
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| author | George Moukarzel Yi Wang Weiyue Xin Carl Hofmann Anjali Joshi John W. Loughney Amy Bowman |
| author_facet | George Moukarzel Yi Wang Weiyue Xin Carl Hofmann Anjali Joshi John W. Loughney Amy Bowman |
| author_sort | George Moukarzel |
| collection | DOAJ |
| description | High Throughput Screening is crucial in pharmaceutical companies for efficient testing in drug discovery and development. Our Vaccines Analytical Research and Development (V-AR&D) department extensively uses robotic liquid handlers in their High Throughput Analytics (HTA) group for assay development and sample screening. However, these instruments are expensive and require extensive training. Opentrons' OT-2 liquid handler offers a more affordable option (<∼ $10,000) with Python programming language and open-source flexibility, reducing training requirements. OT-2 allows broadening of testing capabilities and method transfer without significant capital investments. Two biochemical assays were conducted to assess OT-2′s performance, and it demonstrated accurate pipetting with low covariance compared to Tecan EVO liquid handlers. Though OT-2 has some limitations such as lack of a crash detection system and limited deck space, it is a cost-effective, medium-throughput, and accurate liquid handling tool suitable for early-stage development and method transfer. |
| format | Article |
| id | doaj-art-7735c1ddd07b4c53a55b4aba7c31adef |
| institution | DOAJ |
| issn | 2472-6303 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | SLAS Technology |
| spelling | doaj-art-7735c1ddd07b4c53a55b4aba7c31adef2025-08-20T02:48:57ZengElsevierSLAS Technology2472-63032024-12-0129610020510.1016/j.slast.2024.100205Automation of biochemical assays using an open-sourced, inexpensive robotic liquid handlerGeorge Moukarzel0Yi Wang1Weiyue Xin2Carl Hofmann3Anjali Joshi4John W. Loughney5Amy Bowman6High Throughput Analytics, Analytical Research & Development, Merck & Co. Inc., Rahway, NJ, United StatesHigh Throughput Analytics, Analytical Research & Development, Merck & Co. Inc., Rahway, NJ, United StatesHigh Throughput Analytics, Analytical Research & Development, Merck & Co. Inc., Rahway, NJ, United StatesHigh Throughput Analytics, Analytical Research & Development, Merck & Co. Inc., Rahway, NJ, United StatesHigh Throughput Analytics, Analytical Research & Development, Merck & Co. Inc., Rahway, NJ, United StatesHigh Throughput Analytics, Analytical Research & Development, Merck & Co. Inc., Rahway, NJ, United StatesCorresponding author.; High Throughput Analytics, Analytical Research & Development, Merck & Co. Inc., Rahway, NJ, United StatesHigh Throughput Screening is crucial in pharmaceutical companies for efficient testing in drug discovery and development. Our Vaccines Analytical Research and Development (V-AR&D) department extensively uses robotic liquid handlers in their High Throughput Analytics (HTA) group for assay development and sample screening. However, these instruments are expensive and require extensive training. Opentrons' OT-2 liquid handler offers a more affordable option (<∼ $10,000) with Python programming language and open-source flexibility, reducing training requirements. OT-2 allows broadening of testing capabilities and method transfer without significant capital investments. Two biochemical assays were conducted to assess OT-2′s performance, and it demonstrated accurate pipetting with low covariance compared to Tecan EVO liquid handlers. Though OT-2 has some limitations such as lack of a crash detection system and limited deck space, it is a cost-effective, medium-throughput, and accurate liquid handling tool suitable for early-stage development and method transfer.http://www.sciencedirect.com/science/article/pii/S2472630324000876OpentronsOT-2Liquid handlerBiochemical assayPicoGreenBradford |
| spellingShingle | George Moukarzel Yi Wang Weiyue Xin Carl Hofmann Anjali Joshi John W. Loughney Amy Bowman Automation of biochemical assays using an open-sourced, inexpensive robotic liquid handler SLAS Technology Opentrons OT-2 Liquid handler Biochemical assay PicoGreen Bradford |
| title | Automation of biochemical assays using an open-sourced, inexpensive robotic liquid handler |
| title_full | Automation of biochemical assays using an open-sourced, inexpensive robotic liquid handler |
| title_fullStr | Automation of biochemical assays using an open-sourced, inexpensive robotic liquid handler |
| title_full_unstemmed | Automation of biochemical assays using an open-sourced, inexpensive robotic liquid handler |
| title_short | Automation of biochemical assays using an open-sourced, inexpensive robotic liquid handler |
| title_sort | automation of biochemical assays using an open sourced inexpensive robotic liquid handler |
| topic | Opentrons OT-2 Liquid handler Biochemical assay PicoGreen Bradford |
| url | http://www.sciencedirect.com/science/article/pii/S2472630324000876 |
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