Targeted treatment of hepatocellular carcinoma with aptamer-guided solid lipid nanoparticles loaded with norcantharidin
Liver cancer is a common malignancy in the world, and its incidence and mortality rate are increasing year by year. The disease has a short course and a high mortality rate, posing a serious threat to humanity and health. The objective of this study is to create novel liver-targeted nanoparticles as...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
|
| Series: | Drug Delivery |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/10717544.2025.2519470 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849688089523388416 |
|---|---|
| author | Yilin Xu Min Wang Jing Wu Manshu Zou Donghai Wu Jing Gong Pingjie Wang Hong Yan Xinhua Xia |
| author_facet | Yilin Xu Min Wang Jing Wu Manshu Zou Donghai Wu Jing Gong Pingjie Wang Hong Yan Xinhua Xia |
| author_sort | Yilin Xu |
| collection | DOAJ |
| description | Liver cancer is a common malignancy in the world, and its incidence and mortality rate are increasing year by year. The disease has a short course and a high mortality rate, posing a serious threat to humanity and health. The objective of this study is to create novel liver-targeted nanoparticles as a potential treatment for liver cancer. The aptamer (APS613-1) modified redox-sensitive norcantharidin solid lipid nanoparticles (Apt-PEG2000-ss-NCTD-SLNs) were prepared by emulsified ultrasonic dispersion method and characterized. The tumor targeting, antitumor effect and safety of the nanoparticles were investigated and evaluated in vitro and in vivo. The particle size of Apt-PEG2000-ss-NCTD-SLNs was 87.95 ± 3.32 nm, and the encapsulation efficiency was about 80.74 ± 2.36%, which had good biocompatibility. The results of in vitro experiments showed that, compared with unmodified solid lipid nanoparticles (NCTD-SLNs), Apt-PEG2000-ss-NCTD-SLNs had better targeting for liver tumor cells, and a stronger ability to inhibit cell proliferation and migration, as well as promote cell apoptosis. The in vivo results revealed that Apt-PEG2000-ss-NCTD-SLNs demonstrated good safety and anti-tumor efficacy, and its mechanism was achieved through the inhibition of cell proliferation and induction of apoptosis. The functionalized nanoparticles modified by aptamer APS613-1 can be used for the liver-targeted delivery of antitumor drugs for the treatment of liver cancer, and Apt-PEG2000-ss-NCTD-SLN is a potential drug for the treatment of liver cancer. |
| format | Article |
| id | doaj-art-773159e1e6eb4fc5a411bc6b37bc168f |
| institution | DOAJ |
| issn | 1071-7544 1521-0464 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Drug Delivery |
| spelling | doaj-art-773159e1e6eb4fc5a411bc6b37bc168f2025-08-20T03:22:07ZengTaylor & Francis GroupDrug Delivery1071-75441521-04642025-12-0132110.1080/10717544.2025.2519470Targeted treatment of hepatocellular carcinoma with aptamer-guided solid lipid nanoparticles loaded with norcantharidinYilin Xu0Min Wang1Jing Wu2Manshu Zou3Donghai Wu4Jing Gong5Pingjie Wang6Hong Yan7Xinhua Xia8School of Pharmacy, Hunan University of Chinese Medicine, Changsha, People’s Republic of ChinaThe Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha, People’s Republic of ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, People’s Republic of ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, People’s Republic of ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, People’s Republic of ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, People’s Republic of ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, People’s Republic of ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, People’s Republic of ChinaSchool of Pharmacy, Hunan University of Chinese Medicine, Changsha, People’s Republic of ChinaLiver cancer is a common malignancy in the world, and its incidence and mortality rate are increasing year by year. The disease has a short course and a high mortality rate, posing a serious threat to humanity and health. The objective of this study is to create novel liver-targeted nanoparticles as a potential treatment for liver cancer. The aptamer (APS613-1) modified redox-sensitive norcantharidin solid lipid nanoparticles (Apt-PEG2000-ss-NCTD-SLNs) were prepared by emulsified ultrasonic dispersion method and characterized. The tumor targeting, antitumor effect and safety of the nanoparticles were investigated and evaluated in vitro and in vivo. The particle size of Apt-PEG2000-ss-NCTD-SLNs was 87.95 ± 3.32 nm, and the encapsulation efficiency was about 80.74 ± 2.36%, which had good biocompatibility. The results of in vitro experiments showed that, compared with unmodified solid lipid nanoparticles (NCTD-SLNs), Apt-PEG2000-ss-NCTD-SLNs had better targeting for liver tumor cells, and a stronger ability to inhibit cell proliferation and migration, as well as promote cell apoptosis. The in vivo results revealed that Apt-PEG2000-ss-NCTD-SLNs demonstrated good safety and anti-tumor efficacy, and its mechanism was achieved through the inhibition of cell proliferation and induction of apoptosis. The functionalized nanoparticles modified by aptamer APS613-1 can be used for the liver-targeted delivery of antitumor drugs for the treatment of liver cancer, and Apt-PEG2000-ss-NCTD-SLN is a potential drug for the treatment of liver cancer.https://www.tandfonline.com/doi/10.1080/10717544.2025.2519470Norcantharidinaptamernanoparticlestargeted drug delivery systemhepatocellular carcinoma |
| spellingShingle | Yilin Xu Min Wang Jing Wu Manshu Zou Donghai Wu Jing Gong Pingjie Wang Hong Yan Xinhua Xia Targeted treatment of hepatocellular carcinoma with aptamer-guided solid lipid nanoparticles loaded with norcantharidin Drug Delivery Norcantharidin aptamer nanoparticles targeted drug delivery system hepatocellular carcinoma |
| title | Targeted treatment of hepatocellular carcinoma with aptamer-guided solid lipid nanoparticles loaded with norcantharidin |
| title_full | Targeted treatment of hepatocellular carcinoma with aptamer-guided solid lipid nanoparticles loaded with norcantharidin |
| title_fullStr | Targeted treatment of hepatocellular carcinoma with aptamer-guided solid lipid nanoparticles loaded with norcantharidin |
| title_full_unstemmed | Targeted treatment of hepatocellular carcinoma with aptamer-guided solid lipid nanoparticles loaded with norcantharidin |
| title_short | Targeted treatment of hepatocellular carcinoma with aptamer-guided solid lipid nanoparticles loaded with norcantharidin |
| title_sort | targeted treatment of hepatocellular carcinoma with aptamer guided solid lipid nanoparticles loaded with norcantharidin |
| topic | Norcantharidin aptamer nanoparticles targeted drug delivery system hepatocellular carcinoma |
| url | https://www.tandfonline.com/doi/10.1080/10717544.2025.2519470 |
| work_keys_str_mv | AT yilinxu targetedtreatmentofhepatocellularcarcinomawithaptamerguidedsolidlipidnanoparticlesloadedwithnorcantharidin AT minwang targetedtreatmentofhepatocellularcarcinomawithaptamerguidedsolidlipidnanoparticlesloadedwithnorcantharidin AT jingwu targetedtreatmentofhepatocellularcarcinomawithaptamerguidedsolidlipidnanoparticlesloadedwithnorcantharidin AT manshuzou targetedtreatmentofhepatocellularcarcinomawithaptamerguidedsolidlipidnanoparticlesloadedwithnorcantharidin AT donghaiwu targetedtreatmentofhepatocellularcarcinomawithaptamerguidedsolidlipidnanoparticlesloadedwithnorcantharidin AT jinggong targetedtreatmentofhepatocellularcarcinomawithaptamerguidedsolidlipidnanoparticlesloadedwithnorcantharidin AT pingjiewang targetedtreatmentofhepatocellularcarcinomawithaptamerguidedsolidlipidnanoparticlesloadedwithnorcantharidin AT hongyan targetedtreatmentofhepatocellularcarcinomawithaptamerguidedsolidlipidnanoparticlesloadedwithnorcantharidin AT xinhuaxia targetedtreatmentofhepatocellularcarcinomawithaptamerguidedsolidlipidnanoparticlesloadedwithnorcantharidin |