miR-129-1-3p inhibits osteogenic differentiation of human bone marrow mesenchymal stem cells via BMP2/SMAD1 signaling pathway
Objective To investigate the effect of miR-129-1-3p on the osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) and its potential mechanism. Methods Negative control group, the miR-129-1-3p mimic group, the miR-129-1-3p inhibitor group and the corresponding negative contro...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Stomatology
2025-06-01
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| Series: | Kouqiang yixue |
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| Online Access: | https://www.stomatology.cn/fileup/1003-9872/PDF/1751968115751-948761778.pdf |
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| Summary: | Objective To investigate the effect of miR-129-1-3p on the osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) and its potential mechanism. Methods Negative control group, the miR-129-1-3p mimic group, the miR-129-1-3p inhibitor group and the corresponding negative control were constructed and transfected into hBMSCs. The formation of calcium-mineralized nodules was observed by alkaline phosphatase staining and alizarin red S staining. The expression levels of miR-129-1-3p and osteogenic differentiation markers were detected by qRT-PCR. Western blot detected the protein expressions of bone morphogenetic protein 2 (BMP2), SMAD1 and p-SMAD1. Results After transfection, the expression level of miR-129-1-3p in mimic group was significantly increased (P<0.05), the number of mineralized nodules was significantly decreased, and the expression levels of BMP2, Runt-related transcription factor 2 (RUNX2), osteocalcin (OCN) mRNA were significantly down-regulated (P<0.05). BMP2 and p-SMAD1 protein were also significantly down-regulated (P<0.05) compared with Mimic-NC group. The expression levels of BMP2, RUNX2, OCN mRNA were significantly up-regulated in inhibitor group (P<0.05) compared with Inhibitor-NC group. BMP2 and p-SMAD1 protein were significantly up-regulated in inhibitor group (P<0.05). Conclusion miR-129-1-3p can inhibit the osteogenic differentiation of human bone marrow mesenchymal stem cells by suppressing BMP2/SMAD1 signaling pathway. |
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| ISSN: | 1003-9872 |