Comparative analysis of dietary iron deprivation and supplementation in a murine model of colitis
Abstract Inflammatory bowel diseases are chronic inflammatory conditions with growing prevalence in western populations. Iron is an essential component of erythrocytes hemoglobin. Under the influence of elevated hepcidin production, iron is sequestered in cells during inflammation which, in turn, le...
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| Format: | Article |
| Language: | English |
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Wiley
2025-05-01
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| Series: | FASEB BioAdvances |
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| Online Access: | https://doi.org/10.1096/fba.2025-00022 |
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| author | Thanina Medjbeur Ugo Sardo Prunelle Perrier Kevin Cormier Maryline Roy Anne Dumay Léon Kautz |
| author_facet | Thanina Medjbeur Ugo Sardo Prunelle Perrier Kevin Cormier Maryline Roy Anne Dumay Léon Kautz |
| author_sort | Thanina Medjbeur |
| collection | DOAJ |
| description | Abstract Inflammatory bowel diseases are chronic inflammatory conditions with growing prevalence in western populations. Iron is an essential component of erythrocytes hemoglobin. Under the influence of elevated hepcidin production, iron is sequestered in cells during inflammation which, in turn, leads to iron restriction for red blood cell synthesis. As a consequence, iron deficiency and anemia of inflammation are the most prevalent extraintestinal complications in IBD patients. Iron deficiency is commonly treated with oral iron supplements, with limited efficacy as iron absorption is blunted during intestinal inflammation. Moreover, iron supplementation can cause intestinal complications, as previous studies have shown that it can worsen the inflammatory response. However, a comparative analysis of the effects of low, adequate, and high dietary iron content matching the iron supplementation given to patients has not been performed in mice. We therefore tested the impact of dietary iron deprivation and supplementation in a murine model of colitis induced by dextran sodium sulfate. We found that both dietary iron deprivation and supplementation were accompanied by a more severe inflammation with earlier signs of gastrointestinal bleeding compared to mice fed an iron‐adequate diet. The manipulation of dietary iron led to a profound dysbiosis in the colon of control mice that differed depending on the dietary iron content. Analysis of this dysbiosis is in line with a pronounced susceptibility to colonic inflammation, thus questioning the benefit/risk balance of oral iron supplementation for IBD patients. |
| format | Article |
| id | doaj-art-771b5bae32f0472d8bcdb620bed936f5 |
| institution | OA Journals |
| issn | 2573-9832 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | FASEB BioAdvances |
| spelling | doaj-art-771b5bae32f0472d8bcdb620bed936f52025-08-20T02:11:17ZengWileyFASEB BioAdvances2573-98322025-05-0175n/an/a10.1096/fba.2025-00022Comparative analysis of dietary iron deprivation and supplementation in a murine model of colitisThanina Medjbeur0Ugo Sardo1Prunelle Perrier2Kevin Cormier3Maryline Roy4Anne Dumay5Léon Kautz6IRSD, Université de Toulouse, INSERM, INRAE, ENVT, Univ Toulouse III – Paul Sabatier (UPS) Toulouse FranceIRSD, Université de Toulouse, INSERM, INRAE, ENVT, Univ Toulouse III – Paul Sabatier (UPS) Toulouse FranceIRSD, Université de Toulouse, INSERM, INRAE, ENVT, Univ Toulouse III – Paul Sabatier (UPS) Toulouse FranceIRSD, Université de Toulouse, INSERM, INRAE, ENVT, Univ Toulouse III – Paul Sabatier (UPS) Toulouse FranceUniversité Paris Cité, INSERM UMR1149 and CNRS EMR8252, Centre de Recherche Sur l'Inflammation, Inflamex Laboratory of Excellence Paris FranceUniversité Paris Cité, INSERM UMR1149 and CNRS EMR8252, Centre de Recherche Sur l'Inflammation, Inflamex Laboratory of Excellence Paris FranceIRSD, Université de Toulouse, INSERM, INRAE, ENVT, Univ Toulouse III – Paul Sabatier (UPS) Toulouse FranceAbstract Inflammatory bowel diseases are chronic inflammatory conditions with growing prevalence in western populations. Iron is an essential component of erythrocytes hemoglobin. Under the influence of elevated hepcidin production, iron is sequestered in cells during inflammation which, in turn, leads to iron restriction for red blood cell synthesis. As a consequence, iron deficiency and anemia of inflammation are the most prevalent extraintestinal complications in IBD patients. Iron deficiency is commonly treated with oral iron supplements, with limited efficacy as iron absorption is blunted during intestinal inflammation. Moreover, iron supplementation can cause intestinal complications, as previous studies have shown that it can worsen the inflammatory response. However, a comparative analysis of the effects of low, adequate, and high dietary iron content matching the iron supplementation given to patients has not been performed in mice. We therefore tested the impact of dietary iron deprivation and supplementation in a murine model of colitis induced by dextran sodium sulfate. We found that both dietary iron deprivation and supplementation were accompanied by a more severe inflammation with earlier signs of gastrointestinal bleeding compared to mice fed an iron‐adequate diet. The manipulation of dietary iron led to a profound dysbiosis in the colon of control mice that differed depending on the dietary iron content. Analysis of this dysbiosis is in line with a pronounced susceptibility to colonic inflammation, thus questioning the benefit/risk balance of oral iron supplementation for IBD patients.https://doi.org/10.1096/fba.2025-00022anemiacolitisinflammationironmicrobiota |
| spellingShingle | Thanina Medjbeur Ugo Sardo Prunelle Perrier Kevin Cormier Maryline Roy Anne Dumay Léon Kautz Comparative analysis of dietary iron deprivation and supplementation in a murine model of colitis FASEB BioAdvances anemia colitis inflammation iron microbiota |
| title | Comparative analysis of dietary iron deprivation and supplementation in a murine model of colitis |
| title_full | Comparative analysis of dietary iron deprivation and supplementation in a murine model of colitis |
| title_fullStr | Comparative analysis of dietary iron deprivation and supplementation in a murine model of colitis |
| title_full_unstemmed | Comparative analysis of dietary iron deprivation and supplementation in a murine model of colitis |
| title_short | Comparative analysis of dietary iron deprivation and supplementation in a murine model of colitis |
| title_sort | comparative analysis of dietary iron deprivation and supplementation in a murine model of colitis |
| topic | anemia colitis inflammation iron microbiota |
| url | https://doi.org/10.1096/fba.2025-00022 |
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