Rapid discovery of drug-introduced multiple organ dysfunction via NIR-II fluorescent imaging
The precise and rapid monitoring of multiple organ dysfunction is crucial in drug discovery. Traditional methods, such as pathological analysis, are often time-consuming and inefficient. Here, we developed a multiplexed near-infrared window two (NIR-II) fluorescent bioimaging method that allows for...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-08-01
|
| Series: | Acta Pharmaceutica Sinica B |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211383525004484 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849233547078926336 |
|---|---|
| author | Pu Jiang Ruihu Song Yue Hu Xin He Zewei Zhang Xuemei Wei Zhiming Wang De-an Guo Hao Chen |
| author_facet | Pu Jiang Ruihu Song Yue Hu Xin He Zewei Zhang Xuemei Wei Zhiming Wang De-an Guo Hao Chen |
| author_sort | Pu Jiang |
| collection | DOAJ |
| description | The precise and rapid monitoring of multiple organ dysfunction is crucial in drug discovery. Traditional methods, such as pathological analysis, are often time-consuming and inefficient. Here, we developed a multiplexed near-infrared window two (NIR-II) fluorescent bioimaging method that allows for real-time, rapid, and quantitative assessment of multiple organ dysfunctions. Given that existing probes did not fully meet requirements, we synthesized a range of NIR-II hemicyanine dyes (HDs) with varying absorption and emission wavelengths. By modifying these dyes, we achieved high spatial and temporal resolution imaging of the liver, kidneys, stomach, and intestines. This method was further applied to investigate disorders induced by cisplatin, a drug known to cause gastric emptying issues along with liver and kidney injuries. By monitoring the metabolic rate of the dyes in these organs, we accurately quantified multi-organ dysfunction, which was also confirmed by gold-standard pathological analysis. Additionally, we evaluated the effects of five aristolochic acids (AAs) on multiple organ dysfunction. For the first time, we identified that AA-I and AA-II could cause gastric emptying disorders, which was further validated through transcriptomics analysis. Our study introduces a novel approach for the simultaneous monitoring of multi-organ dysfunction, which may significantly enhance the evaluation of drug side effects. |
| format | Article |
| id | doaj-art-771b26deb841439297e0a87f85284e33 |
| institution | Kabale University |
| issn | 2211-3835 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Acta Pharmaceutica Sinica B |
| spelling | doaj-art-771b26deb841439297e0a87f85284e332025-08-20T05:06:40ZengElsevierActa Pharmaceutica Sinica B2211-38352025-08-011584285429910.1016/j.apsb.2025.06.023Rapid discovery of drug-introduced multiple organ dysfunction via NIR-II fluorescent imagingPu Jiang0Ruihu Song1Yue Hu2Xin He3Zewei Zhang4Xuemei Wei5Zhiming Wang6De-an Guo7Hao Chen8School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China; Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center of TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; State Key Laboratory of Chemical Biology, Molecular Imaging Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaState Key Laboratory of Chemical Biology, Molecular Imaging Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China; State Key Laboratory of Chemical Biology, Molecular Imaging Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China; Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center of TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; State Key Laboratory of Chemical Biology, Molecular Imaging Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China; State Key Laboratory of Chemical Biology, Molecular Imaging Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaState Key Laboratory of Chemical Biology, Molecular Imaging Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaState Key Laboratory of Chemical Biology, Molecular Imaging Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; Corresponding authors.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China; Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center of TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; Corresponding authors.School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China; State Key Laboratory of Chemical Biology, Molecular Imaging Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; Corresponding authors.The precise and rapid monitoring of multiple organ dysfunction is crucial in drug discovery. Traditional methods, such as pathological analysis, are often time-consuming and inefficient. Here, we developed a multiplexed near-infrared window two (NIR-II) fluorescent bioimaging method that allows for real-time, rapid, and quantitative assessment of multiple organ dysfunctions. Given that existing probes did not fully meet requirements, we synthesized a range of NIR-II hemicyanine dyes (HDs) with varying absorption and emission wavelengths. By modifying these dyes, we achieved high spatial and temporal resolution imaging of the liver, kidneys, stomach, and intestines. This method was further applied to investigate disorders induced by cisplatin, a drug known to cause gastric emptying issues along with liver and kidney injuries. By monitoring the metabolic rate of the dyes in these organs, we accurately quantified multi-organ dysfunction, which was also confirmed by gold-standard pathological analysis. Additionally, we evaluated the effects of five aristolochic acids (AAs) on multiple organ dysfunction. For the first time, we identified that AA-I and AA-II could cause gastric emptying disorders, which was further validated through transcriptomics analysis. Our study introduces a novel approach for the simultaneous monitoring of multi-organ dysfunction, which may significantly enhance the evaluation of drug side effects.http://www.sciencedirect.com/science/article/pii/S2211383525004484Drug side effectMultiplexed imagingAristolochic acidsNIR-II imagingHemicyanine dyesLiver injuries |
| spellingShingle | Pu Jiang Ruihu Song Yue Hu Xin He Zewei Zhang Xuemei Wei Zhiming Wang De-an Guo Hao Chen Rapid discovery of drug-introduced multiple organ dysfunction via NIR-II fluorescent imaging Acta Pharmaceutica Sinica B Drug side effect Multiplexed imaging Aristolochic acids NIR-II imaging Hemicyanine dyes Liver injuries |
| title | Rapid discovery of drug-introduced multiple organ dysfunction via NIR-II fluorescent imaging |
| title_full | Rapid discovery of drug-introduced multiple organ dysfunction via NIR-II fluorescent imaging |
| title_fullStr | Rapid discovery of drug-introduced multiple organ dysfunction via NIR-II fluorescent imaging |
| title_full_unstemmed | Rapid discovery of drug-introduced multiple organ dysfunction via NIR-II fluorescent imaging |
| title_short | Rapid discovery of drug-introduced multiple organ dysfunction via NIR-II fluorescent imaging |
| title_sort | rapid discovery of drug introduced multiple organ dysfunction via nir ii fluorescent imaging |
| topic | Drug side effect Multiplexed imaging Aristolochic acids NIR-II imaging Hemicyanine dyes Liver injuries |
| url | http://www.sciencedirect.com/science/article/pii/S2211383525004484 |
| work_keys_str_mv | AT pujiang rapiddiscoveryofdrugintroducedmultipleorgandysfunctionvianiriifluorescentimaging AT ruihusong rapiddiscoveryofdrugintroducedmultipleorgandysfunctionvianiriifluorescentimaging AT yuehu rapiddiscoveryofdrugintroducedmultipleorgandysfunctionvianiriifluorescentimaging AT xinhe rapiddiscoveryofdrugintroducedmultipleorgandysfunctionvianiriifluorescentimaging AT zeweizhang rapiddiscoveryofdrugintroducedmultipleorgandysfunctionvianiriifluorescentimaging AT xuemeiwei rapiddiscoveryofdrugintroducedmultipleorgandysfunctionvianiriifluorescentimaging AT zhimingwang rapiddiscoveryofdrugintroducedmultipleorgandysfunctionvianiriifluorescentimaging AT deanguo rapiddiscoveryofdrugintroducedmultipleorgandysfunctionvianiriifluorescentimaging AT haochen rapiddiscoveryofdrugintroducedmultipleorgandysfunctionvianiriifluorescentimaging |