Histological Evaluation of Subcutaneous Tissue Reactions to a Novel Bilayer Polycaprolactone/Silk Fibroin/Strontium Carbonate Nanofibrous Membrane for Guided Bone Regeneration: A Study in Rabbits
ABSTRACT Objectives The selection of appropriate biomaterial for guided bone regeneration is challenging. The blending of polymers is a simple method to retain their characteristics and to compensate for the drawbacks of each component. The release of Sr+2 (strontium) ions from the polycaprolactone/...
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Wiley
2025-06-01
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| Series: | Clinical and Experimental Dental Research |
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| Online Access: | https://doi.org/10.1002/cre2.70140 |
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| author | Lida Kheiri Arash Golestaneh Mehdi Mehdikhani Sayed Mohammad Razavi Niloofar Etemadi |
| author_facet | Lida Kheiri Arash Golestaneh Mehdi Mehdikhani Sayed Mohammad Razavi Niloofar Etemadi |
| author_sort | Lida Kheiri |
| collection | DOAJ |
| description | ABSTRACT Objectives The selection of appropriate biomaterial for guided bone regeneration is challenging. The blending of polymers is a simple method to retain their characteristics and to compensate for the drawbacks of each component. The release of Sr+2 (strontium) ions from the polycaprolactone/strontium carbonate (PCL/SrC) nanocomposite is the main reason of enhanced osteogenesis. The most important reasons of clinical failure after using biomaterials include infections and lack of tissue‐integration. Modifications of silk fibroin (SF)–based membranes improved new bone formation in animal studies without inflammatory reaction. The aim of the present study was to compare biological response of the subcutaneous connective tissue to a novel bilayer PCL (60 wt%)/SF (20 wt%)/SrC (20 wt%) membrane to a commercially available collagenous membrane. Material and Methods Eighteen male New Zealand rabbits were randomly divided into three groups, and all received subcutaneously the following materials: novel bilayer membrane, commercial membrane, and empty defect as control group, which were tested after 7, 14, and 28 days. The type and severity of inflammation, granulation tissue, and fibrous tissue were assessed. Results The connective tissue surrounding the implanted samples of each group exhibited the presence of similar cells close to the control groups. Statistical analyses showed no significant differences between the specimens in each time period. Conclusions In general, the novel bilayer nanocomposite membrane was a biocompatible material and produces a similar subcutaneous response compared to commercially available membrane. Besides, it demonstrated promise for guided bone regeneration technique for treating the osseous defects of oral and maxillofacial region. |
| format | Article |
| id | doaj-art-77170187fa244a8ab2ef3cb16a69337a |
| institution | DOAJ |
| issn | 2057-4347 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Wiley |
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| series | Clinical and Experimental Dental Research |
| spelling | doaj-art-77170187fa244a8ab2ef3cb16a69337a2025-08-20T03:04:49ZengWileyClinical and Experimental Dental Research2057-43472025-06-01113n/an/a10.1002/cre2.70140Histological Evaluation of Subcutaneous Tissue Reactions to a Novel Bilayer Polycaprolactone/Silk Fibroin/Strontium Carbonate Nanofibrous Membrane for Guided Bone Regeneration: A Study in RabbitsLida Kheiri0Arash Golestaneh1Mehdi Mehdikhani2Sayed Mohammad Razavi3Niloofar Etemadi4Department of Oral and Maxillofacial Surgery School of Dentistry, Islamic Azad University, Isfahan (Khorasgan) Branch Isfahan IranDepartment of Oral and Maxillofacial Surgery School of Dentistry, Islamic Azad University, Isfahan (Khorasgan) Branch Isfahan IranDepartment of Biomedical Engineering, Faculty of Engineering University of Isfahan Isfahan IranDepartment of Oral and Maxillofacial Pathology, Dental Implants Research Center School of Dentistry, Isfahan University of Medical Sciences Isfahan IranDepartment of Materials Engineering, Najafabad Branch Islamic Azad University Najafabad IranABSTRACT Objectives The selection of appropriate biomaterial for guided bone regeneration is challenging. The blending of polymers is a simple method to retain their characteristics and to compensate for the drawbacks of each component. The release of Sr+2 (strontium) ions from the polycaprolactone/strontium carbonate (PCL/SrC) nanocomposite is the main reason of enhanced osteogenesis. The most important reasons of clinical failure after using biomaterials include infections and lack of tissue‐integration. Modifications of silk fibroin (SF)–based membranes improved new bone formation in animal studies without inflammatory reaction. The aim of the present study was to compare biological response of the subcutaneous connective tissue to a novel bilayer PCL (60 wt%)/SF (20 wt%)/SrC (20 wt%) membrane to a commercially available collagenous membrane. Material and Methods Eighteen male New Zealand rabbits were randomly divided into three groups, and all received subcutaneously the following materials: novel bilayer membrane, commercial membrane, and empty defect as control group, which were tested after 7, 14, and 28 days. The type and severity of inflammation, granulation tissue, and fibrous tissue were assessed. Results The connective tissue surrounding the implanted samples of each group exhibited the presence of similar cells close to the control groups. Statistical analyses showed no significant differences between the specimens in each time period. Conclusions In general, the novel bilayer nanocomposite membrane was a biocompatible material and produces a similar subcutaneous response compared to commercially available membrane. Besides, it demonstrated promise for guided bone regeneration technique for treating the osseous defects of oral and maxillofacial region.https://doi.org/10.1002/cre2.70140bilayer membranepolycaprolactonesilk fibroinstrontium carbonate |
| spellingShingle | Lida Kheiri Arash Golestaneh Mehdi Mehdikhani Sayed Mohammad Razavi Niloofar Etemadi Histological Evaluation of Subcutaneous Tissue Reactions to a Novel Bilayer Polycaprolactone/Silk Fibroin/Strontium Carbonate Nanofibrous Membrane for Guided Bone Regeneration: A Study in Rabbits Clinical and Experimental Dental Research bilayer membrane polycaprolactone silk fibroin strontium carbonate |
| title | Histological Evaluation of Subcutaneous Tissue Reactions to a Novel Bilayer Polycaprolactone/Silk Fibroin/Strontium Carbonate Nanofibrous Membrane for Guided Bone Regeneration: A Study in Rabbits |
| title_full | Histological Evaluation of Subcutaneous Tissue Reactions to a Novel Bilayer Polycaprolactone/Silk Fibroin/Strontium Carbonate Nanofibrous Membrane for Guided Bone Regeneration: A Study in Rabbits |
| title_fullStr | Histological Evaluation of Subcutaneous Tissue Reactions to a Novel Bilayer Polycaprolactone/Silk Fibroin/Strontium Carbonate Nanofibrous Membrane for Guided Bone Regeneration: A Study in Rabbits |
| title_full_unstemmed | Histological Evaluation of Subcutaneous Tissue Reactions to a Novel Bilayer Polycaprolactone/Silk Fibroin/Strontium Carbonate Nanofibrous Membrane for Guided Bone Regeneration: A Study in Rabbits |
| title_short | Histological Evaluation of Subcutaneous Tissue Reactions to a Novel Bilayer Polycaprolactone/Silk Fibroin/Strontium Carbonate Nanofibrous Membrane for Guided Bone Regeneration: A Study in Rabbits |
| title_sort | histological evaluation of subcutaneous tissue reactions to a novel bilayer polycaprolactone silk fibroin strontium carbonate nanofibrous membrane for guided bone regeneration a study in rabbits |
| topic | bilayer membrane polycaprolactone silk fibroin strontium carbonate |
| url | https://doi.org/10.1002/cre2.70140 |
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