3D Printing of Photocrosslinked Alginate Dialdehyde‐Gelatin Hydrogels Reinforced with Cobalt‐Containing Mesoporous Bioactive Glass Nanoparticles for Developing Skin Wound Dressings

Abstract Designing three‐dimensional (3D)‐printable hydrogels presents a significant challenge, particularly in maintaining the polymer concentration at minimal levels while ensuring that the printing process can be cell‐friendly at room temperature. This study aims to examine the feasibility of des...

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Main Authors: Ehsan Zeimaran, Sara Pourshahrestani, Jonas Röder, Rainer Detsch, Aldo R. Boccaccini
Format: Article
Language:English
Published: Wiley-VCH 2025-06-01
Series:Advanced Materials Interfaces
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Online Access:https://doi.org/10.1002/admi.202400913
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author Ehsan Zeimaran
Sara Pourshahrestani
Jonas Röder
Rainer Detsch
Aldo R. Boccaccini
author_facet Ehsan Zeimaran
Sara Pourshahrestani
Jonas Röder
Rainer Detsch
Aldo R. Boccaccini
author_sort Ehsan Zeimaran
collection DOAJ
description Abstract Designing three‐dimensional (3D)‐printable hydrogels presents a significant challenge, particularly in maintaining the polymer concentration at minimal levels while ensuring that the printing process can be cell‐friendly at room temperature. This study aims to examine the feasibility of designing multi‐crosslinked hydrogels composed of methacrylate‐functionalized alginate dialdehyde (ADAMA) and different concentrations of gelatin methacrylate (GelMA). Cobalt‐doped mesoporous bioactive glass nanoparticles (Co‐MBGNs) are incorporated into the hydrogels to further tailor their mechanical and biological properties. The hydrogels, crosslinked under UV and immersed in calcium chloride solution, show high elasticity and fatigue resistance over five compression cycles. The inclusion of Co‐MBGNs enhances the mechanical properties while cell viability is higher in the pure polymeric hydrogels. The nanocomposites promote cell aggregate formation. The non‐cytotoxic hydrogel supernatants induce NHDF cell migration at rates similar to or even faster than the control group. The printability of hydrogels is also examined through extrusion‐bioprinting approach. The room‐temperature printing is only possible for the hydrogels made of 5 wt% of GelMA and 2.5 wt% of ADAMA. The 3D‐printed constructs show impressive elasticity and are easy to handle. These findings can offer valuable insights into the development of mechanically robust and customizable wound dressings.
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institution Kabale University
issn 2196-7350
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series Advanced Materials Interfaces
spelling doaj-art-7713fba767ed4a2da1f75c504e9eee992025-08-20T03:45:49ZengWiley-VCHAdvanced Materials Interfaces2196-73502025-06-011211n/an/a10.1002/admi.2024009133D Printing of Photocrosslinked Alginate Dialdehyde‐Gelatin Hydrogels Reinforced with Cobalt‐Containing Mesoporous Bioactive Glass Nanoparticles for Developing Skin Wound DressingsEhsan Zeimaran0Sara Pourshahrestani1Jonas Röder2Rainer Detsch3Aldo R. Boccaccini4Institute of Biomaterials Friedrich‐Alexander‐Universität Erlangen‐Nürnberg Cauerstrasse 6 91058 Erlangen GermanyInstitute of Biomaterials Friedrich‐Alexander‐Universität Erlangen‐Nürnberg Cauerstrasse 6 91058 Erlangen GermanyInstitute of Biomaterials Friedrich‐Alexander‐Universität Erlangen‐Nürnberg Cauerstrasse 6 91058 Erlangen GermanyInstitute of Biomaterials Friedrich‐Alexander‐Universität Erlangen‐Nürnberg Cauerstrasse 6 91058 Erlangen GermanyInstitute of Biomaterials Friedrich‐Alexander‐Universität Erlangen‐Nürnberg Cauerstrasse 6 91058 Erlangen GermanyAbstract Designing three‐dimensional (3D)‐printable hydrogels presents a significant challenge, particularly in maintaining the polymer concentration at minimal levels while ensuring that the printing process can be cell‐friendly at room temperature. This study aims to examine the feasibility of designing multi‐crosslinked hydrogels composed of methacrylate‐functionalized alginate dialdehyde (ADAMA) and different concentrations of gelatin methacrylate (GelMA). Cobalt‐doped mesoporous bioactive glass nanoparticles (Co‐MBGNs) are incorporated into the hydrogels to further tailor their mechanical and biological properties. The hydrogels, crosslinked under UV and immersed in calcium chloride solution, show high elasticity and fatigue resistance over five compression cycles. The inclusion of Co‐MBGNs enhances the mechanical properties while cell viability is higher in the pure polymeric hydrogels. The nanocomposites promote cell aggregate formation. The non‐cytotoxic hydrogel supernatants induce NHDF cell migration at rates similar to or even faster than the control group. The printability of hydrogels is also examined through extrusion‐bioprinting approach. The room‐temperature printing is only possible for the hydrogels made of 5 wt% of GelMA and 2.5 wt% of ADAMA. The 3D‐printed constructs show impressive elasticity and are easy to handle. These findings can offer valuable insights into the development of mechanically robust and customizable wound dressings.https://doi.org/10.1002/admi.202400913GelMAmesoporous bioactive glass nanoparticlesmethacrylated alginate dialdehydeprintable hydrogelswound healing
spellingShingle Ehsan Zeimaran
Sara Pourshahrestani
Jonas Röder
Rainer Detsch
Aldo R. Boccaccini
3D Printing of Photocrosslinked Alginate Dialdehyde‐Gelatin Hydrogels Reinforced with Cobalt‐Containing Mesoporous Bioactive Glass Nanoparticles for Developing Skin Wound Dressings
Advanced Materials Interfaces
GelMA
mesoporous bioactive glass nanoparticles
methacrylated alginate dialdehyde
printable hydrogels
wound healing
title 3D Printing of Photocrosslinked Alginate Dialdehyde‐Gelatin Hydrogels Reinforced with Cobalt‐Containing Mesoporous Bioactive Glass Nanoparticles for Developing Skin Wound Dressings
title_full 3D Printing of Photocrosslinked Alginate Dialdehyde‐Gelatin Hydrogels Reinforced with Cobalt‐Containing Mesoporous Bioactive Glass Nanoparticles for Developing Skin Wound Dressings
title_fullStr 3D Printing of Photocrosslinked Alginate Dialdehyde‐Gelatin Hydrogels Reinforced with Cobalt‐Containing Mesoporous Bioactive Glass Nanoparticles for Developing Skin Wound Dressings
title_full_unstemmed 3D Printing of Photocrosslinked Alginate Dialdehyde‐Gelatin Hydrogels Reinforced with Cobalt‐Containing Mesoporous Bioactive Glass Nanoparticles for Developing Skin Wound Dressings
title_short 3D Printing of Photocrosslinked Alginate Dialdehyde‐Gelatin Hydrogels Reinforced with Cobalt‐Containing Mesoporous Bioactive Glass Nanoparticles for Developing Skin Wound Dressings
title_sort 3d printing of photocrosslinked alginate dialdehyde gelatin hydrogels reinforced with cobalt containing mesoporous bioactive glass nanoparticles for developing skin wound dressings
topic GelMA
mesoporous bioactive glass nanoparticles
methacrylated alginate dialdehyde
printable hydrogels
wound healing
url https://doi.org/10.1002/admi.202400913
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