Assessment of three-dimensional biofilm structure using an optical microscope
A method allowing the evaluation of the structure and the calculation of the volume of a biofilm, using an optical microscope, is proposed based on the linear relation between the intensity of a pixel in biofilm images grabbed on the x-y plane and the corresponding number of cells in the z direction...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2007-05-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/000112403 |
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| _version_ | 1850152070768754688 |
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| author | Carla C.C.R. de Carvalho M. Manuela R. da Fonseca |
| author_facet | Carla C.C.R. de Carvalho M. Manuela R. da Fonseca |
| author_sort | Carla C.C.R. de Carvalho |
| collection | DOAJ |
| description | A method allowing the evaluation of the structure and the calculation of the volume of a biofilm, using an optical microscope, is proposed based on the linear relation between the intensity of a pixel in biofilm images grabbed on the x-y plane and the corresponding number of cells in the z direction, which allows the calculation of the biofilm thickness. The method is intended to overcome the need for expensive microscopes to study biofilms. |
| format | Article |
| id | doaj-art-77120aa7fa8a4adca3c3384c10a5789e |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2007-05-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-77120aa7fa8a4adca3c3384c10a5789e2025-08-20T02:26:04ZengTaylor & Francis GroupBioTechniques0736-62051940-98182007-05-0142561662010.2144/000112403Assessment of three-dimensional biofilm structure using an optical microscopeCarla C.C.R. de Carvalho0M. Manuela R. da Fonseca11Instituto Superior Técnico, Lisbon, Portugal1Instituto Superior Técnico, Lisbon, PortugalA method allowing the evaluation of the structure and the calculation of the volume of a biofilm, using an optical microscope, is proposed based on the linear relation between the intensity of a pixel in biofilm images grabbed on the x-y plane and the corresponding number of cells in the z direction, which allows the calculation of the biofilm thickness. The method is intended to overcome the need for expensive microscopes to study biofilms.https://www.future-science.com/doi/10.2144/000112403 |
| spellingShingle | Carla C.C.R. de Carvalho M. Manuela R. da Fonseca Assessment of three-dimensional biofilm structure using an optical microscope BioTechniques |
| title | Assessment of three-dimensional biofilm structure using an optical microscope |
| title_full | Assessment of three-dimensional biofilm structure using an optical microscope |
| title_fullStr | Assessment of three-dimensional biofilm structure using an optical microscope |
| title_full_unstemmed | Assessment of three-dimensional biofilm structure using an optical microscope |
| title_short | Assessment of three-dimensional biofilm structure using an optical microscope |
| title_sort | assessment of three dimensional biofilm structure using an optical microscope |
| url | https://www.future-science.com/doi/10.2144/000112403 |
| work_keys_str_mv | AT carlaccrdecarvalho assessmentofthreedimensionalbiofilmstructureusinganopticalmicroscope AT mmanuelardafonseca assessmentofthreedimensionalbiofilmstructureusinganopticalmicroscope |