Molecular Detection of CRISPR-Cas System in Staphylococcus epidermidis Isolated from Different Sources in Iraq
The goal of the present study was to isolate and identify (CRISPR-Cas) elements in S. epidermidis (MRSE) from 300 samples collected from various sources. Culture techniques, biochemical tests and VITEK2 system were used for confirming of Staphylococcus epidermidis isolation. The partial se...
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College of Veterinary Medicine, University of Basrah, Iraq
2022-06-01
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| Series: | Basrah Journal of Veterinary Research |
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| Online Access: | https://bjvr.uobasrah.edu.iq/article_174826_8a8bb41b2f7bb1e25f9c2399bb0bbba3.pdf |
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| author | Thuraya Hashosh et al |
| author_facet | Thuraya Hashosh et al |
| author_sort | Thuraya Hashosh et al |
| collection | DOAJ |
| description | The goal of the present study was to isolate and identify (CRISPR-Cas) elements in S. epidermidis (MRSE) from 300 samples collected from various sources. Culture techniques, biochemical tests and VITEK2 system were used for confirming of Staphylococcus epidermidis isolation. The partial sequencing of the 16s rRNA gene was evaluated and compared with those in the Gene Bank to find differences in the sequence using the BLAST tool (http://www.ncbi.nlm.nih.gov). On the other hand, the CRISPR-Cas system was examined in all isolates of multidrug-resistant S. epidermidis in which the SECR1 and SECR2 elements were not found. The cas6 element was found in all of the tested bacterial strains. Susceptibility tests were done using the disc diffusion method after identification. Antibiotic resistance testing on S. epidermidis isolates revealed the highest percentage of multi-resistance to ampicillin, ceftriaxone, chloramphenicol, cefalexin, vancomycin, nalidixic acid at 100%. Moreover, S. epidermidis isolates showed intermediate effect against amoxillin-clavulanic acid, tobramycin, neomycin, oxacillin, tetracycline and methicillin. However, S. epidermidis isolates were found to be the most sensitive to erythromycin, norfloxacin and rifampicin. The CRISPR-Cas system can be found in S. epidermidis isolates from a variety of local sources and that the appearance of the CRISPR system in S. epidermidis isolates plays a unique role in antibiotic suitability. |
| format | Article |
| id | doaj-art-76f05f1187bc4cbc8455e0006dd3cc3a |
| institution | DOAJ |
| issn | 1813-8497 2410-8456 |
| language | English |
| publishDate | 2022-06-01 |
| publisher | College of Veterinary Medicine, University of Basrah, Iraq |
| record_format | Article |
| series | Basrah Journal of Veterinary Research |
| spelling | doaj-art-76f05f1187bc4cbc8455e0006dd3cc3a2025-08-20T03:09:55ZengCollege of Veterinary Medicine, University of Basrah, IraqBasrah Journal of Veterinary Research1813-84972410-84562022-06-01212153010.23975/bjvetr.2022.174826174826Molecular Detection of CRISPR-Cas System in Staphylococcus epidermidis Isolated from Different Sources in IraqThuraya Hashosh et alThe goal of the present study was to isolate and identify (CRISPR-Cas) elements in S. epidermidis (MRSE) from 300 samples collected from various sources. Culture techniques, biochemical tests and VITEK2 system were used for confirming of Staphylococcus epidermidis isolation. The partial sequencing of the 16s rRNA gene was evaluated and compared with those in the Gene Bank to find differences in the sequence using the BLAST tool (http://www.ncbi.nlm.nih.gov). On the other hand, the CRISPR-Cas system was examined in all isolates of multidrug-resistant S. epidermidis in which the SECR1 and SECR2 elements were not found. The cas6 element was found in all of the tested bacterial strains. Susceptibility tests were done using the disc diffusion method after identification. Antibiotic resistance testing on S. epidermidis isolates revealed the highest percentage of multi-resistance to ampicillin, ceftriaxone, chloramphenicol, cefalexin, vancomycin, nalidixic acid at 100%. Moreover, S. epidermidis isolates showed intermediate effect against amoxillin-clavulanic acid, tobramycin, neomycin, oxacillin, tetracycline and methicillin. However, S. epidermidis isolates were found to be the most sensitive to erythromycin, norfloxacin and rifampicin. The CRISPR-Cas system can be found in S. epidermidis isolates from a variety of local sources and that the appearance of the CRISPR system in S. epidermidis isolates plays a unique role in antibiotic suitability.https://bjvr.uobasrah.edu.iq/article_174826_8a8bb41b2f7bb1e25f9c2399bb0bbba3.pdfstaphylococcus epidermidiscrispr-cas systemcas 6secr1 and secr2 |
| spellingShingle | Thuraya Hashosh et al Molecular Detection of CRISPR-Cas System in Staphylococcus epidermidis Isolated from Different Sources in Iraq Basrah Journal of Veterinary Research staphylococcus epidermidis crispr-cas system cas 6 secr1 and secr2 |
| title | Molecular Detection of CRISPR-Cas System in Staphylococcus epidermidis Isolated from Different Sources in Iraq |
| title_full | Molecular Detection of CRISPR-Cas System in Staphylococcus epidermidis Isolated from Different Sources in Iraq |
| title_fullStr | Molecular Detection of CRISPR-Cas System in Staphylococcus epidermidis Isolated from Different Sources in Iraq |
| title_full_unstemmed | Molecular Detection of CRISPR-Cas System in Staphylococcus epidermidis Isolated from Different Sources in Iraq |
| title_short | Molecular Detection of CRISPR-Cas System in Staphylococcus epidermidis Isolated from Different Sources in Iraq |
| title_sort | molecular detection of crispr cas system in staphylococcus epidermidis isolated from different sources in iraq |
| topic | staphylococcus epidermidis crispr-cas system cas 6 secr1 and secr2 |
| url | https://bjvr.uobasrah.edu.iq/article_174826_8a8bb41b2f7bb1e25f9c2399bb0bbba3.pdf |
| work_keys_str_mv | AT thurayahashoshetal moleculardetectionofcrisprcassysteminstaphylococcusepidermidisisolatedfromdifferentsourcesiniraq |