Antibody response to the epsilon toxin of Clostridium perfringens following vaccination of Lama glama crias
BACKGROUND: Enterotoxaemia produced by Clostridium perfringens A, C and D is an important cause of mortality in young llamas. There is no data on antibody responses following vaccination with epsilon toxin. METHODOLOGY: Twenty-six L. glama crias were divided into four groups which were vaccinated wi...
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| Format: | Article |
| Language: | English |
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The Journal of Infection in Developing Countries
2009-09-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/555 |
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| author | Adriana B. Bentancor Pablo Halperin Myriam Flores Fabián Iribarren |
| author_facet | Adriana B. Bentancor Pablo Halperin Myriam Flores Fabián Iribarren |
| author_sort | Adriana B. Bentancor |
| collection | DOAJ |
| description | BACKGROUND: Enterotoxaemia produced by Clostridium perfringens A, C and D is an important cause of mortality in young llamas. There is no data on antibody responses following vaccination with epsilon toxin.
METHODOLOGY: Twenty-six L. glama crias were divided into four groups which were vaccinated with a commercial vaccine (Mancha Gangrena Enterotoxemia, Instituto Rosembusch Sociedad Anónima, Argentina) on days 0, 21 and 42 or left as unvaccinated controls. An indirect ELISA was compared with the mouse neutralization test (MNT) for measuring titers to C. perfringens type D epsilon toxin and used to determine titers in sera taken before vaccination and 16, 28, 49, 59, and 93 days later.
RESULTS: The ELISA gave comparable results to the MNT and showed animals vaccinated once failed to develop raised titers. A week following a second vaccination, mean antibody titers rose significantly (P < 0.05) and 7/12 animals developed high titers which were present in only one animal at the end of the study (day 93). A third vaccination resulted in a decrease in mean antibody titers a week later.
CONCLUSIONS: Llamas develop antibodies to Clostridium perfringens type D epsilon toxin after two vaccinations at a 21-day interval. Further studies are indicated to determine if these inoculations protect against enterotoxemia and the most appropriate vaccination schedule. |
| format | Article |
| id | doaj-art-76eb0534576a4fd2b5a3e7b7516da9d1 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2009-09-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-76eb0534576a4fd2b5a3e7b7516da9d12025-08-20T03:48:58ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802009-09-0130810.3855/jidc.555Antibody response to the epsilon toxin of Clostridium perfringens following vaccination of Lama glama criasAdriana B. Bentancor0Pablo Halperin1Myriam Flores2Fabián Iribarren3Microbiología, Facultad de Ciencias Veterinarias Universidad de Buenos Aires, Argentina, Chorroarín 280. Cdad. Autónoma de Buenos AiresHistología, Facultad de Ciencias Veterinarias Universidad de Buenos Aires, Argentina, Chorroarín 280. Cdad. Autónoma de Buenos AiresEstadística, Facultad de Ciencias Veterinarias Universidad de Buenos Aires, Argentina, Chorroarín 280. Cdad. Autónoma de Buenos AiresEnfermedades Infecciosas, Facultad de Ciencias Veterinarias Universidad de Buenos Aires, Argentina, Chorroarín 280. Cdad. Autónoma de Buenos AiresBACKGROUND: Enterotoxaemia produced by Clostridium perfringens A, C and D is an important cause of mortality in young llamas. There is no data on antibody responses following vaccination with epsilon toxin. METHODOLOGY: Twenty-six L. glama crias were divided into four groups which were vaccinated with a commercial vaccine (Mancha Gangrena Enterotoxemia, Instituto Rosembusch Sociedad Anónima, Argentina) on days 0, 21 and 42 or left as unvaccinated controls. An indirect ELISA was compared with the mouse neutralization test (MNT) for measuring titers to C. perfringens type D epsilon toxin and used to determine titers in sera taken before vaccination and 16, 28, 49, 59, and 93 days later. RESULTS: The ELISA gave comparable results to the MNT and showed animals vaccinated once failed to develop raised titers. A week following a second vaccination, mean antibody titers rose significantly (P < 0.05) and 7/12 animals developed high titers which were present in only one animal at the end of the study (day 93). A third vaccination resulted in a decrease in mean antibody titers a week later. CONCLUSIONS: Llamas develop antibodies to Clostridium perfringens type D epsilon toxin after two vaccinations at a 21-day interval. Further studies are indicated to determine if these inoculations protect against enterotoxemia and the most appropriate vaccination schedule.https://jidc.org/index.php/journal/article/view/555Lama glamaC. perfringens type D epsilon toxinantibodiesvaccine |
| spellingShingle | Adriana B. Bentancor Pablo Halperin Myriam Flores Fabián Iribarren Antibody response to the epsilon toxin of Clostridium perfringens following vaccination of Lama glama crias Journal of Infection in Developing Countries Lama glama C. perfringens type D epsilon toxin antibodies vaccine |
| title | Antibody response to the epsilon toxin of Clostridium perfringens following vaccination of Lama glama crias |
| title_full | Antibody response to the epsilon toxin of Clostridium perfringens following vaccination of Lama glama crias |
| title_fullStr | Antibody response to the epsilon toxin of Clostridium perfringens following vaccination of Lama glama crias |
| title_full_unstemmed | Antibody response to the epsilon toxin of Clostridium perfringens following vaccination of Lama glama crias |
| title_short | Antibody response to the epsilon toxin of Clostridium perfringens following vaccination of Lama glama crias |
| title_sort | antibody response to the epsilon toxin of clostridium perfringens following vaccination of lama glama crias |
| topic | Lama glama C. perfringens type D epsilon toxin antibodies vaccine |
| url | https://jidc.org/index.php/journal/article/view/555 |
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