Effect of ovarian dysfunction induced ovariectomy and Porphyromonas gingivalis induction to risk of metabolic syndrome: in vivo study

Periodontal diseases and metabolic syndrome are related to complicated multifactorial conditions. However, the relationship is not yet evident. Estrogen insufficiency might correlate to this condition, possibly caused by ovarian removal and Porphyromonas gingivalis (P. gingivalis) infection. This s...

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Main Authors: Agustin Wulan Suci-Dharmayanti, Zahara Meilawaty, Tantin Ermawati, Agus Murdojohadi Putradjaka, Zahreni Hamzah
Format: Article
Language:English
Published: Universidade Estadual Paulista 2024-06-01
Series:Brazilian Dental Science
Online Access:https://ojs.ict.unesp.br/index.php/cob/article/view/3880
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author Agustin Wulan Suci-Dharmayanti
Zahara Meilawaty
Tantin Ermawati
Agus Murdojohadi Putradjaka
Zahreni Hamzah
author_facet Agustin Wulan Suci-Dharmayanti
Zahara Meilawaty
Tantin Ermawati
Agus Murdojohadi Putradjaka
Zahreni Hamzah
author_sort Agustin Wulan Suci-Dharmayanti
collection DOAJ
description Periodontal diseases and metabolic syndrome are related to complicated multifactorial conditions. However, the relationship is not yet evident. Estrogen insufficiency might correlate to this condition, possibly caused by ovarian removal and Porphyromonas gingivalis (P. gingivalis) infection. This study aimed to evaluate the effect of ovarian dysfunction caused by ovariectomy and P. gingivalis infection to metabolic syndrome development. This study was an experimental laboratory study using female rats Sprague Dawley Strain. Animal models were divided into four groups: control, ovariectomy (OVX), ovariectomy-periodontitis (OPG), and periodontitis (PG). The purpose of every treatment in each group was to induce ovarian dysfunction. The OVX group was undertaken ovaries removal surgery. PG was performed P. gingivalis induction. Therefore OPG was a combination of ovariectomy and P. gingivalis induction. Blood was drawn and observed on days 0, 3, 7, 14, 21, and 28. The blood sample was examined for uric acid, cholesterol, glucose and estrogen. The collected data were all statistically examined. All treatment groups presented body weight and blood biochemical observation significantly higher than the control group, except total cholesterol (p<0.05). Moreover, most variables presented a correlation between groups to body weight and biochemical blood indicators, except blood uric acid level (R>0.5). The metabolic syndrome was triggered by ovarian dysfunction brought on by P. gingivalis infection after ovariectomy. They both took the same risk. Even P. gingivalis induction made metabolic syndrome in the group of animal models which underwent ovariectomy worse. KEYWORDS Estrogen deficiency; Metabolic syndrome; Ovariectomy; Ovarian dysfunction; P. gingivalis.
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spelling doaj-art-76e2aa5348fc4c33a3b647990442cd402025-08-22T14:49:11ZengUniversidade Estadual PaulistaBrazilian Dental Science2178-60112024-06-0127110.4322/bds.2024.e3880Effect of ovarian dysfunction induced ovariectomy and Porphyromonas gingivalis induction to risk of metabolic syndrome: in vivo studyAgustin Wulan Suci-Dharmayanti0https://orcid.org/0000-0003-3037-7055Zahara Meilawaty1https://orcid.org/0000-0001-7709-4576Tantin Ermawati2https://orcid.org/0000-0002-0322-8997Agus Murdojohadi Putradjaka3https://orcid.org/0009-0006-2261-8647Zahreni Hamzah4https://orcid.org/0000-0001-5101-0872Universitas Jember, Biomedical Department, Faculty of Dentistry. Jember, Indonesia.Universitas Jember, Biomedical Department, Faculty of Dentistry. Jember, Indonesia.Universitas Jember, Biomedical Department, Faculty of Dentistry. Jember, Indonesia.Universitas Jember, Biomedical Department, Faculty of Dentistry. Jember, Indonesia.Universitas Jember, Biomedical Department, Faculty of Dentistry. Jember, Indonesia. Periodontal diseases and metabolic syndrome are related to complicated multifactorial conditions. However, the relationship is not yet evident. Estrogen insufficiency might correlate to this condition, possibly caused by ovarian removal and Porphyromonas gingivalis (P. gingivalis) infection. This study aimed to evaluate the effect of ovarian dysfunction caused by ovariectomy and P. gingivalis infection to metabolic syndrome development. This study was an experimental laboratory study using female rats Sprague Dawley Strain. Animal models were divided into four groups: control, ovariectomy (OVX), ovariectomy-periodontitis (OPG), and periodontitis (PG). The purpose of every treatment in each group was to induce ovarian dysfunction. The OVX group was undertaken ovaries removal surgery. PG was performed P. gingivalis induction. Therefore OPG was a combination of ovariectomy and P. gingivalis induction. Blood was drawn and observed on days 0, 3, 7, 14, 21, and 28. The blood sample was examined for uric acid, cholesterol, glucose and estrogen. The collected data were all statistically examined. All treatment groups presented body weight and blood biochemical observation significantly higher than the control group, except total cholesterol (p<0.05). Moreover, most variables presented a correlation between groups to body weight and biochemical blood indicators, except blood uric acid level (R>0.5). The metabolic syndrome was triggered by ovarian dysfunction brought on by P. gingivalis infection after ovariectomy. They both took the same risk. Even P. gingivalis induction made metabolic syndrome in the group of animal models which underwent ovariectomy worse. KEYWORDS Estrogen deficiency; Metabolic syndrome; Ovariectomy; Ovarian dysfunction; P. gingivalis. https://ojs.ict.unesp.br/index.php/cob/article/view/3880
spellingShingle Agustin Wulan Suci-Dharmayanti
Zahara Meilawaty
Tantin Ermawati
Agus Murdojohadi Putradjaka
Zahreni Hamzah
Effect of ovarian dysfunction induced ovariectomy and Porphyromonas gingivalis induction to risk of metabolic syndrome: in vivo study
Brazilian Dental Science
title Effect of ovarian dysfunction induced ovariectomy and Porphyromonas gingivalis induction to risk of metabolic syndrome: in vivo study
title_full Effect of ovarian dysfunction induced ovariectomy and Porphyromonas gingivalis induction to risk of metabolic syndrome: in vivo study
title_fullStr Effect of ovarian dysfunction induced ovariectomy and Porphyromonas gingivalis induction to risk of metabolic syndrome: in vivo study
title_full_unstemmed Effect of ovarian dysfunction induced ovariectomy and Porphyromonas gingivalis induction to risk of metabolic syndrome: in vivo study
title_short Effect of ovarian dysfunction induced ovariectomy and Porphyromonas gingivalis induction to risk of metabolic syndrome: in vivo study
title_sort effect of ovarian dysfunction induced ovariectomy and porphyromonas gingivalis induction to risk of metabolic syndrome in vivo study
url https://ojs.ict.unesp.br/index.php/cob/article/view/3880
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