The effect of hyperbaric oxygen and platelet-rich plasma treatments on the adipogenic C2C12 myoblast cell line
Abstract Background Platelet-rich plasma (PRP) and hyperbaric oxygen (HBO) treatments have been explored for their potential effects on muscle and adipose tissue. However, the impact of these treatments on the proliferation and differentiation of adipogenic C2C12 myoblasts, as well as the expression...
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| Format: | Article |
| Language: | English |
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BMC
2025-07-01
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| Series: | BMC Musculoskeletal Disorders |
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| Online Access: | https://doi.org/10.1186/s12891-025-08930-3 |
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| author | Joe Chih-Hao Chiu Poyu Chen Kin Fong Lei Gwo-Jyh Chang Su-Ching Chen Rei Higashikawa Yi-Sheng Chan |
| author_facet | Joe Chih-Hao Chiu Poyu Chen Kin Fong Lei Gwo-Jyh Chang Su-Ching Chen Rei Higashikawa Yi-Sheng Chan |
| author_sort | Joe Chih-Hao Chiu |
| collection | DOAJ |
| description | Abstract Background Platelet-rich plasma (PRP) and hyperbaric oxygen (HBO) treatments have been explored for their potential effects on muscle and adipose tissue. However, the impact of these treatments on the proliferation and differentiation of adipogenic C2C12 myoblasts, as well as the expression of muscle-related and adipose-related genes, remains unclear. Purpose This study aimed to investigate the effects of PRP and HBO treatments on the proliferation and differentiation of adipogenic C2C12 myoblasts and to examine the expression patterns of muscle-related and adipose-related genes in response to these treatments. Methods Adipogenic C2C12 myoblasts were subjected to three courses of HBO treatment (25 min of 100% O2 following 5 min of air at 1.5 ATA) and different PRP treatments (leukocyte-rich PRP, LR-PRP; and leukocyte-poor PRP, LP-PRP). Cell proliferation rates were assessed at various time points. The expression of muscle-related genes (MYF5 and Myogenin) and adipose-related genes (Adipo-Q, FABP4, and PPARy) was analyzed using RT-PCR. Results HBO treatment alone did not stimulate myoblast proliferation, and cells exposed to HBO had lower proliferation rates compared to control. PRP treatments significantly increased cell proliferation, regardless of HBO co-treatment. Cells exposed to both HBO and PRPs had significantly lower proliferation rates than those treated with PRPs alone, suggesting that HBO negatively affects adipogenic C2C12 myoblast proliferation. The inhibitory effect of HBO was significantly alleviated by concomitant LR-PRP and LP-PRP treatments on day 5 (both P <.001). Muscle-related genes showed non-significant trends in expression after treatments, while adipose-related genes exhibited varying expression patterns, but none were statistically significant compared to control. Conclusions PRP treatments significantly increase C2C12 myoblast proliferation, while HBO treatment alone has a negative effect on proliferation. The combination of PRP and HBO treatments can alleviate the inhibitory effect of HBO. The expression of muscle-related and adipose-related genes did not show statistically significant changes in response to the treatments. |
| format | Article |
| id | doaj-art-76de6dc13d4846dfaca725edeefda80b |
| institution | Kabale University |
| issn | 1471-2474 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Musculoskeletal Disorders |
| spelling | doaj-art-76de6dc13d4846dfaca725edeefda80b2025-08-20T04:01:43ZengBMCBMC Musculoskeletal Disorders1471-24742025-07-0126111010.1186/s12891-025-08930-3The effect of hyperbaric oxygen and platelet-rich plasma treatments on the adipogenic C2C12 myoblast cell lineJoe Chih-Hao Chiu0Poyu Chen1Kin Fong Lei2Gwo-Jyh Chang3Su-Ching Chen4Rei Higashikawa5Yi-Sheng Chan6Department of Orthopedic Surgery, Linkou Chang Gung Memorial HospitalDepartment of Orthopedic Surgery, Linkou Chang Gung Memorial HospitalChang Gung University Graduate Institute of Biomedical SciencesChang Gung University College of EngineeringDepartment of Orthopedic Surgery, Linkou Chang Gung Memorial HospitalDepartment of Orthopedic Surgery, Linkou Chang Gung Memorial HospitalBone and Joint Research Center, Chang Gung Memorial HospitalAbstract Background Platelet-rich plasma (PRP) and hyperbaric oxygen (HBO) treatments have been explored for their potential effects on muscle and adipose tissue. However, the impact of these treatments on the proliferation and differentiation of adipogenic C2C12 myoblasts, as well as the expression of muscle-related and adipose-related genes, remains unclear. Purpose This study aimed to investigate the effects of PRP and HBO treatments on the proliferation and differentiation of adipogenic C2C12 myoblasts and to examine the expression patterns of muscle-related and adipose-related genes in response to these treatments. Methods Adipogenic C2C12 myoblasts were subjected to three courses of HBO treatment (25 min of 100% O2 following 5 min of air at 1.5 ATA) and different PRP treatments (leukocyte-rich PRP, LR-PRP; and leukocyte-poor PRP, LP-PRP). Cell proliferation rates were assessed at various time points. The expression of muscle-related genes (MYF5 and Myogenin) and adipose-related genes (Adipo-Q, FABP4, and PPARy) was analyzed using RT-PCR. Results HBO treatment alone did not stimulate myoblast proliferation, and cells exposed to HBO had lower proliferation rates compared to control. PRP treatments significantly increased cell proliferation, regardless of HBO co-treatment. Cells exposed to both HBO and PRPs had significantly lower proliferation rates than those treated with PRPs alone, suggesting that HBO negatively affects adipogenic C2C12 myoblast proliferation. The inhibitory effect of HBO was significantly alleviated by concomitant LR-PRP and LP-PRP treatments on day 5 (both P <.001). Muscle-related genes showed non-significant trends in expression after treatments, while adipose-related genes exhibited varying expression patterns, but none were statistically significant compared to control. Conclusions PRP treatments significantly increase C2C12 myoblast proliferation, while HBO treatment alone has a negative effect on proliferation. The combination of PRP and HBO treatments can alleviate the inhibitory effect of HBO. The expression of muscle-related and adipose-related genes did not show statistically significant changes in response to the treatments.https://doi.org/10.1186/s12891-025-08930-3Hyperbaric oxygen treatmentPlatelet-rich plasmaMyoblastsAdipogenesis |
| spellingShingle | Joe Chih-Hao Chiu Poyu Chen Kin Fong Lei Gwo-Jyh Chang Su-Ching Chen Rei Higashikawa Yi-Sheng Chan The effect of hyperbaric oxygen and platelet-rich plasma treatments on the adipogenic C2C12 myoblast cell line BMC Musculoskeletal Disorders Hyperbaric oxygen treatment Platelet-rich plasma Myoblasts Adipogenesis |
| title | The effect of hyperbaric oxygen and platelet-rich plasma treatments on the adipogenic C2C12 myoblast cell line |
| title_full | The effect of hyperbaric oxygen and platelet-rich plasma treatments on the adipogenic C2C12 myoblast cell line |
| title_fullStr | The effect of hyperbaric oxygen and platelet-rich plasma treatments on the adipogenic C2C12 myoblast cell line |
| title_full_unstemmed | The effect of hyperbaric oxygen and platelet-rich plasma treatments on the adipogenic C2C12 myoblast cell line |
| title_short | The effect of hyperbaric oxygen and platelet-rich plasma treatments on the adipogenic C2C12 myoblast cell line |
| title_sort | effect of hyperbaric oxygen and platelet rich plasma treatments on the adipogenic c2c12 myoblast cell line |
| topic | Hyperbaric oxygen treatment Platelet-rich plasma Myoblasts Adipogenesis |
| url | https://doi.org/10.1186/s12891-025-08930-3 |
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