Unraveling the impact of human cerebrospinal fluid on human neural stem cell fate
Human neural stem/progenitor cells (hNSCs) can potentially treat neurological diseases, but their low survival and proliferation rates after transplantation remain challenging. In our study, we preincubated hNSCs with the human cerebrospinal fluid (CSF) to obtain closer to the physiological brain en...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-03-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2025.1527557/full |
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| author | Klaudia Radoszkiewicz Aleksandra Bzinkowska Monika Sypecka Dorota Sulejczak Daniela Ferrari Maurizio Gelati Angelo Luigi Vescovi Angelo Luigi Vescovi Anna Sarnowska |
| author_facet | Klaudia Radoszkiewicz Aleksandra Bzinkowska Monika Sypecka Dorota Sulejczak Daniela Ferrari Maurizio Gelati Angelo Luigi Vescovi Angelo Luigi Vescovi Anna Sarnowska |
| author_sort | Klaudia Radoszkiewicz |
| collection | DOAJ |
| description | Human neural stem/progenitor cells (hNSCs) can potentially treat neurological diseases, but their low survival and proliferation rates after transplantation remain challenging. In our study, we preincubated hNSCs with the human cerebrospinal fluid (CSF) to obtain closer to the physiological brain environment and to assess NSC fate and their therapeutic abilities in vitro, ex vivo, and in vivo. We observed significant changes in the differentiation, migratory, and secretory potential of CSF-treated hNSCs, as well as their elevated neuroprotective potential after co-culture with ischemically damaged by oxygen-glucose deprivation (OGD) organotypic rat hippocampal slices culture (OHC) in comparison to the cells cultured in the standard conditions. Next, we investigated their survival and anti-inflammatory abilities in an in vivo ouabain-induced stroke model. This study highlighted and confirmed the critical importance of nutritional supplementation in maintaining NSC culture and enhancing its therapeutic properties. |
| format | Article |
| id | doaj-art-76daaee688ff4b1b84c798277d042cdc |
| institution | DOAJ |
| issn | 2296-634X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj-art-76daaee688ff4b1b84c798277d042cdc2025-08-20T02:57:07ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2025-03-011310.3389/fcell.2025.15275571527557Unraveling the impact of human cerebrospinal fluid on human neural stem cell fateKlaudia Radoszkiewicz0Aleksandra Bzinkowska1Monika Sypecka2Dorota Sulejczak3Daniela Ferrari4Maurizio Gelati5Angelo Luigi Vescovi6Angelo Luigi Vescovi7Anna Sarnowska8Translational Platform for Regenerative Medicine, Mossakowski Medical Research Institute, Polish Academy of Sciences, Warsaw, PolandTranslational Platform for Regenerative Medicine, Mossakowski Medical Research Institute, Polish Academy of Sciences, Warsaw, PolandTranslational Platform for Regenerative Medicine, Mossakowski Medical Research Institute, Polish Academy of Sciences, Warsaw, PolandDepartment of Experimental Pharmacology, Mossakowski Medical Research Institute, Polish Academy of Sciences, Warsaw, PolandDepartment of Biotechnology and Biosciences, University of Milano-Bicocca, Milan, ItalyUPTA Unit Fondazione IRCCS Casa Sollievo della Sofferenza, Foggia, ItalyDepartment of Biotechnology and Biosciences, University of Milano-Bicocca, Milan, ItalyIRCCS Casa Sollievo della Sofferenza, Foggia, ItalyTranslational Platform for Regenerative Medicine, Mossakowski Medical Research Institute, Polish Academy of Sciences, Warsaw, PolandHuman neural stem/progenitor cells (hNSCs) can potentially treat neurological diseases, but their low survival and proliferation rates after transplantation remain challenging. In our study, we preincubated hNSCs with the human cerebrospinal fluid (CSF) to obtain closer to the physiological brain environment and to assess NSC fate and their therapeutic abilities in vitro, ex vivo, and in vivo. We observed significant changes in the differentiation, migratory, and secretory potential of CSF-treated hNSCs, as well as their elevated neuroprotective potential after co-culture with ischemically damaged by oxygen-glucose deprivation (OGD) organotypic rat hippocampal slices culture (OHC) in comparison to the cells cultured in the standard conditions. Next, we investigated their survival and anti-inflammatory abilities in an in vivo ouabain-induced stroke model. This study highlighted and confirmed the critical importance of nutritional supplementation in maintaining NSC culture and enhancing its therapeutic properties.https://www.frontiersin.org/articles/10.3389/fcell.2025.1527557/fullneural stem cellsischemic strokecerebrospinal fluidneuroprotectioncell therapy |
| spellingShingle | Klaudia Radoszkiewicz Aleksandra Bzinkowska Monika Sypecka Dorota Sulejczak Daniela Ferrari Maurizio Gelati Angelo Luigi Vescovi Angelo Luigi Vescovi Anna Sarnowska Unraveling the impact of human cerebrospinal fluid on human neural stem cell fate Frontiers in Cell and Developmental Biology neural stem cells ischemic stroke cerebrospinal fluid neuroprotection cell therapy |
| title | Unraveling the impact of human cerebrospinal fluid on human neural stem cell fate |
| title_full | Unraveling the impact of human cerebrospinal fluid on human neural stem cell fate |
| title_fullStr | Unraveling the impact of human cerebrospinal fluid on human neural stem cell fate |
| title_full_unstemmed | Unraveling the impact of human cerebrospinal fluid on human neural stem cell fate |
| title_short | Unraveling the impact of human cerebrospinal fluid on human neural stem cell fate |
| title_sort | unraveling the impact of human cerebrospinal fluid on human neural stem cell fate |
| topic | neural stem cells ischemic stroke cerebrospinal fluid neuroprotection cell therapy |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2025.1527557/full |
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