Phenotypic and molecular characterization of potential pathogens from raw fish, meat and milk samples sold and consumed in Calabar Metropolis, Cross River State, Nigeria
Objectives: This study isolated and characterized potential pathogens from raw fish, meat (goat and ram), and milk samples using cultural and molecular techniques, and their susceptibility to routine antibiotics. Methods: This design was a cross-sectional study where raw fish, meat (goat and ram)...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Babcock Medical Society
2024-12-01
|
| Series: | Babcock University Medical Journal |
| Subjects: | |
| Online Access: | http://bumj.babcock.edu.ng/index.php/bumj/article/view/528 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841558973860282368 |
|---|---|
| author | Joy Chinweokwu Ugwu Glory Philemon Bebia Edet Uwem Ajaba Mathias Okang Emmanuel O. Ibeneme Andrew Chijioke Ugwu Augustine Agorye Unimke Francisca O. Nwaokorie James Epoke |
| author_facet | Joy Chinweokwu Ugwu Glory Philemon Bebia Edet Uwem Ajaba Mathias Okang Emmanuel O. Ibeneme Andrew Chijioke Ugwu Augustine Agorye Unimke Francisca O. Nwaokorie James Epoke |
| author_sort | Joy Chinweokwu Ugwu |
| collection | DOAJ |
| description |
Objectives: This study isolated and characterized potential pathogens from raw fish, meat (goat and ram), and milk samples using cultural and molecular techniques, and their susceptibility to routine antibiotics.
Methods: This design was a cross-sectional study where raw fish, meat (goat and ram), and milk from various markets were characterised using cultural and molecular susceptibility to antibiotics used to manage infections they cause in clinical use. Furthermore, the isolates were subjected to pathogenicity tests using amylase and protease screening.
Results: Cultural technique identified a total of 42 isolates and these were: Morganella sp (n=6), Providencia sp (n=7), Klebsiella sp (n=10), E. coli (n=11) and Salmonella sp (n=8). All the isolates showed multi-drug resistance (MDR) to gentamicin, levofloxacin, clindamycin, ciprofloxacin, cefoxicin, piperacillin-azobactam, amikacin, and amoxicillin-clavulanic acid antibiotics. Following antimicrobial sensitivity, 9 isolates with multidrug resistance were selected for molecular characterisation and these were identified as Escherichia coli, Klebsiella pneumoniae, Morganella morganii subsp. Morganii, Providencia stuartii, Klebsiella pneumoniae and Salmonella enterica subsp. Enterica. All the isolates showed amylase and protease activity.
Conclusion: The MDR and pathogenicity potential of the isolates indicate their ability to elicit a potential foodborne infection in the study area and it is a cause for concern.
|
| format | Article |
| id | doaj-art-76d83d72660a487ba4731daf9b0a9091 |
| institution | Kabale University |
| issn | 2465-6666 2756-4657 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Babcock Medical Society |
| record_format | Article |
| series | Babcock University Medical Journal |
| spelling | doaj-art-76d83d72660a487ba4731daf9b0a90912025-01-05T18:31:34ZengBabcock Medical SocietyBabcock University Medical Journal2465-66662756-46572024-12-017210.38029/babcockuniv.med.j..v7i2.528Phenotypic and molecular characterization of potential pathogens from raw fish, meat and milk samples sold and consumed in Calabar Metropolis, Cross River State, NigeriaJoy Chinweokwu Ugwu0Glory Philemon Bebia1Edet Uwem2Ajaba Mathias Okang3Emmanuel O. Ibeneme4Andrew Chijioke Ugwu5Augustine Agorye Unimke6Francisca O. Nwaokorie7James Epoke8Department of Microbiology, Faculty of Biological Science, University of Calabar; Calabar; NigeriaDepartment of Biological (Microbiology), Faculty of Natural and Applied Sciences, Arthur Jarvis, University, Akpabuyo, Cross River State, NigeriaDepartment of Biological (Microbiology), Faculty of Natural and Applied Sciences, Arthur Jarvis, University, Akpabuyo, Cross River State, Nigeria Department of Science Laboratory Technology, University of Calabar; Calabar; NigeriaDepartment of Medical Bacteriology, Virology and Mycology, University of Calabar, Calabar; NigeriaDepartment of Microbiology, Faculty of Biological Science, University of Calabar; Calabar; NigeriaDepartment of Microbiology, Faculty of Biological Science, University of Calabar; Calabar; NigeriaDepartment of Medical Laboratory Science, College of Medicine, University of Lagos, Lagos, NigeriaDepartment of Medical Microbiology/Parasitology, Faculty of Medical Laboratory Science University of Calabar; Nigeria Objectives: This study isolated and characterized potential pathogens from raw fish, meat (goat and ram), and milk samples using cultural and molecular techniques, and their susceptibility to routine antibiotics. Methods: This design was a cross-sectional study where raw fish, meat (goat and ram), and milk from various markets were characterised using cultural and molecular susceptibility to antibiotics used to manage infections they cause in clinical use. Furthermore, the isolates were subjected to pathogenicity tests using amylase and protease screening. Results: Cultural technique identified a total of 42 isolates and these were: Morganella sp (n=6), Providencia sp (n=7), Klebsiella sp (n=10), E. coli (n=11) and Salmonella sp (n=8). All the isolates showed multi-drug resistance (MDR) to gentamicin, levofloxacin, clindamycin, ciprofloxacin, cefoxicin, piperacillin-azobactam, amikacin, and amoxicillin-clavulanic acid antibiotics. Following antimicrobial sensitivity, 9 isolates with multidrug resistance were selected for molecular characterisation and these were identified as Escherichia coli, Klebsiella pneumoniae, Morganella morganii subsp. Morganii, Providencia stuartii, Klebsiella pneumoniae and Salmonella enterica subsp. Enterica. All the isolates showed amylase and protease activity. Conclusion: The MDR and pathogenicity potential of the isolates indicate their ability to elicit a potential foodborne infection in the study area and it is a cause for concern. http://bumj.babcock.edu.ng/index.php/bumj/article/view/528FoodPathogensResistanceAntibioticsCalabarNigeria |
| spellingShingle | Joy Chinweokwu Ugwu Glory Philemon Bebia Edet Uwem Ajaba Mathias Okang Emmanuel O. Ibeneme Andrew Chijioke Ugwu Augustine Agorye Unimke Francisca O. Nwaokorie James Epoke Phenotypic and molecular characterization of potential pathogens from raw fish, meat and milk samples sold and consumed in Calabar Metropolis, Cross River State, Nigeria Babcock University Medical Journal Food Pathogens Resistance Antibiotics Calabar Nigeria |
| title | Phenotypic and molecular characterization of potential pathogens from raw fish, meat and milk samples sold and consumed in Calabar Metropolis, Cross River State, Nigeria |
| title_full | Phenotypic and molecular characterization of potential pathogens from raw fish, meat and milk samples sold and consumed in Calabar Metropolis, Cross River State, Nigeria |
| title_fullStr | Phenotypic and molecular characterization of potential pathogens from raw fish, meat and milk samples sold and consumed in Calabar Metropolis, Cross River State, Nigeria |
| title_full_unstemmed | Phenotypic and molecular characterization of potential pathogens from raw fish, meat and milk samples sold and consumed in Calabar Metropolis, Cross River State, Nigeria |
| title_short | Phenotypic and molecular characterization of potential pathogens from raw fish, meat and milk samples sold and consumed in Calabar Metropolis, Cross River State, Nigeria |
| title_sort | phenotypic and molecular characterization of potential pathogens from raw fish meat and milk samples sold and consumed in calabar metropolis cross river state nigeria |
| topic | Food Pathogens Resistance Antibiotics Calabar Nigeria |
| url | http://bumj.babcock.edu.ng/index.php/bumj/article/view/528 |
| work_keys_str_mv | AT joychinweokwuugwu phenotypicandmolecularcharacterizationofpotentialpathogensfromrawfishmeatandmilksamplessoldandconsumedincalabarmetropoliscrossriverstatenigeria AT gloryphilemonbebia phenotypicandmolecularcharacterizationofpotentialpathogensfromrawfishmeatandmilksamplessoldandconsumedincalabarmetropoliscrossriverstatenigeria AT edetuwem phenotypicandmolecularcharacterizationofpotentialpathogensfromrawfishmeatandmilksamplessoldandconsumedincalabarmetropoliscrossriverstatenigeria AT ajabamathiasokang phenotypicandmolecularcharacterizationofpotentialpathogensfromrawfishmeatandmilksamplessoldandconsumedincalabarmetropoliscrossriverstatenigeria AT emmanueloibeneme phenotypicandmolecularcharacterizationofpotentialpathogensfromrawfishmeatandmilksamplessoldandconsumedincalabarmetropoliscrossriverstatenigeria AT andrewchijiokeugwu phenotypicandmolecularcharacterizationofpotentialpathogensfromrawfishmeatandmilksamplessoldandconsumedincalabarmetropoliscrossriverstatenigeria AT augustineagoryeunimke phenotypicandmolecularcharacterizationofpotentialpathogensfromrawfishmeatandmilksamplessoldandconsumedincalabarmetropoliscrossriverstatenigeria AT franciscaonwaokorie phenotypicandmolecularcharacterizationofpotentialpathogensfromrawfishmeatandmilksamplessoldandconsumedincalabarmetropoliscrossriverstatenigeria AT jamesepoke phenotypicandmolecularcharacterizationofpotentialpathogensfromrawfishmeatandmilksamplessoldandconsumedincalabarmetropoliscrossriverstatenigeria |