Expression and clinical significance of PD-L1, B7-H3, B7-H4 and VISTA in craniopharyngioma
Background Craniopharyngioma (CP) is a common refractory tumor of the central nervous system. However, little is known about the expression and clinical significance of B7 family ligands/receptors in CP patients. Thus, we conducted the present study to address this issue in a cohort of 132 CP cases....
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| Format: | Article |
| Language: | English |
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BMJ Publishing Group
2020-10-01
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| Series: | Journal for ImmunoTherapy of Cancer |
| Online Access: | https://jitc.bmj.com/content/8/2/e000406.full |
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| author | Lin Wang Jianguo Xu Yuelong Wang Shasha Zhao Xin Tang Liangxue Zhou Aiping Tong Jinlong Yang Jiaojiao Deng Tingyue Zhou Zerong Tian Jinhao Yang Hongxu Chen |
| author_facet | Lin Wang Jianguo Xu Yuelong Wang Shasha Zhao Xin Tang Liangxue Zhou Aiping Tong Jinlong Yang Jiaojiao Deng Tingyue Zhou Zerong Tian Jinhao Yang Hongxu Chen |
| author_sort | Lin Wang |
| collection | DOAJ |
| description | Background Craniopharyngioma (CP) is a common refractory tumor of the central nervous system. However, little is known about the expression and clinical significance of B7 family ligands/receptors in CP patients. Thus, we conducted the present study to address this issue in a cohort of 132 CP cases.Methods We mapped and quantified the expression of B7 family ligands/receptors molecules programmed cell death ligand 1 (PD-L1), B7-H3, B7-H4 and V-domain Ig-containing suppressor of T cell activation (VISTA) in 89 adamantinomatous-type CP and 43 papillary-type CP samples using immunohistochemistry and immunofluorescence. Associations between the marker levels, clinicopathological variables and survival were evaluated.Results The positive rates of PD-L1, B7-H3, B7-H4 and VISTA in the cohort of 132 CP cases were 76.5%, 100%, 40.2% and 80.3%, respectively. The cut-off values of PD-L1, B7-H3, B7-H4 and PD-L1 expression were determined by survival receiver operating characteristic (ROC) package, which was 70, 182, 0 and 20, respectively. Elevated expressions of PD-L1, B7-H3, B7-H4 and VISTA were significantly associated with some clinicopathological characteristics. Kaplan-Meier analysis indicated that higher VISTA expressions correlated with better overall survival (OS) and progression-free survival (PFS) (p=0.0053 and p=0.0066, respectively). Multivariate Cox regression analysis indicated that VISTA was an independent prognostic factor for OS (p=0.018) but not for PFS (p=0.898).Conclusions We found variable expression of PD-L1, B7-H3, B7-H4 and VISTA proteins in CPs. The results suggest that the expression level of VISTA may be used as an important indicator to predict the OS and PFS of CPs. B7 family ligands/receptors could be potential immunotherapeutic targets when treating CPs. |
| format | Article |
| id | doaj-art-76d04783fe81490fa7cf2879e72284d4 |
| institution | Kabale University |
| issn | 2051-1426 |
| language | English |
| publishDate | 2020-10-01 |
| publisher | BMJ Publishing Group |
| record_format | Article |
| series | Journal for ImmunoTherapy of Cancer |
| spelling | doaj-art-76d04783fe81490fa7cf2879e72284d42024-11-10T15:35:08ZengBMJ Publishing GroupJournal for ImmunoTherapy of Cancer2051-14262020-10-018210.1136/jitc-2019-000406Expression and clinical significance of PD-L1, B7-H3, B7-H4 and VISTA in craniopharyngiomaLin Wang0Jianguo Xu1Yuelong Wang2Shasha Zhao3Xin Tang4Liangxue Zhou5Aiping Tong6Jinlong Yang7Jiaojiao Deng8Tingyue Zhou9Zerong Tian10Jinhao Yang11Hongxu Chen12Shenzhen Blood Center, Shen Zhen, Guangdong, China1 Evidence-Based Medicine Center, Lanzhou University, Lanzhou, Gansu, ChinaDepartment of Neurosurgery, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaState Key Laboratory of Biotherapy and Cancer Center, West China Hospital, and Collaborative Innovation Center of Biotherapy, Sichuan University, Chengdu, Sichuan, China1 Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu, ChinaDepartment of Neurosurgery, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaState Key Laboratory of Biotherapy and Cancer Center, Research Unit of Gene and Immunotherapy, Chinese Academy of Medical Sciences, Collaborative Innovation Center of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China3BGI Research, Kunming 650106, China3 Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China2 State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, China1 Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu, China1 Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu, China1 Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu, ChinaBackground Craniopharyngioma (CP) is a common refractory tumor of the central nervous system. However, little is known about the expression and clinical significance of B7 family ligands/receptors in CP patients. Thus, we conducted the present study to address this issue in a cohort of 132 CP cases.Methods We mapped and quantified the expression of B7 family ligands/receptors molecules programmed cell death ligand 1 (PD-L1), B7-H3, B7-H4 and V-domain Ig-containing suppressor of T cell activation (VISTA) in 89 adamantinomatous-type CP and 43 papillary-type CP samples using immunohistochemistry and immunofluorescence. Associations between the marker levels, clinicopathological variables and survival were evaluated.Results The positive rates of PD-L1, B7-H3, B7-H4 and VISTA in the cohort of 132 CP cases were 76.5%, 100%, 40.2% and 80.3%, respectively. The cut-off values of PD-L1, B7-H3, B7-H4 and PD-L1 expression were determined by survival receiver operating characteristic (ROC) package, which was 70, 182, 0 and 20, respectively. Elevated expressions of PD-L1, B7-H3, B7-H4 and VISTA were significantly associated with some clinicopathological characteristics. Kaplan-Meier analysis indicated that higher VISTA expressions correlated with better overall survival (OS) and progression-free survival (PFS) (p=0.0053 and p=0.0066, respectively). Multivariate Cox regression analysis indicated that VISTA was an independent prognostic factor for OS (p=0.018) but not for PFS (p=0.898).Conclusions We found variable expression of PD-L1, B7-H3, B7-H4 and VISTA proteins in CPs. The results suggest that the expression level of VISTA may be used as an important indicator to predict the OS and PFS of CPs. B7 family ligands/receptors could be potential immunotherapeutic targets when treating CPs.https://jitc.bmj.com/content/8/2/e000406.full |
| spellingShingle | Lin Wang Jianguo Xu Yuelong Wang Shasha Zhao Xin Tang Liangxue Zhou Aiping Tong Jinlong Yang Jiaojiao Deng Tingyue Zhou Zerong Tian Jinhao Yang Hongxu Chen Expression and clinical significance of PD-L1, B7-H3, B7-H4 and VISTA in craniopharyngioma Journal for ImmunoTherapy of Cancer |
| title | Expression and clinical significance of PD-L1, B7-H3, B7-H4 and VISTA in craniopharyngioma |
| title_full | Expression and clinical significance of PD-L1, B7-H3, B7-H4 and VISTA in craniopharyngioma |
| title_fullStr | Expression and clinical significance of PD-L1, B7-H3, B7-H4 and VISTA in craniopharyngioma |
| title_full_unstemmed | Expression and clinical significance of PD-L1, B7-H3, B7-H4 and VISTA in craniopharyngioma |
| title_short | Expression and clinical significance of PD-L1, B7-H3, B7-H4 and VISTA in craniopharyngioma |
| title_sort | expression and clinical significance of pd l1 b7 h3 b7 h4 and vista in craniopharyngioma |
| url | https://jitc.bmj.com/content/8/2/e000406.full |
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