Protective Role of Catechin and Quercetin in Sodium Benzoate-Induced Lipid Peroxidation and the Antioxidant System in Human Erythrocytes In Vitro
The aim of this study was to evaluate the protective effect of catechin and quercetin in sodium benzoate- (SB-) induced oxidative stress in human erythrocytes in vitro. For this, the effects of SB (6.25, 12.5, 25, 50, and 100 μg/mL), catechin (10 μM), and quercetin (10 μM) on lipid peroxidation (LPO...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/874824 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832568084007747584 |
|---|---|
| author | Gamze Yetuk Dilek Pandir Hatice Bas |
| author_facet | Gamze Yetuk Dilek Pandir Hatice Bas |
| author_sort | Gamze Yetuk |
| collection | DOAJ |
| description | The aim of this study was to evaluate the protective effect of catechin and quercetin in sodium benzoate- (SB-) induced oxidative stress in human erythrocytes in vitro. For this, the effects of SB (6.25, 12.5, 25, 50, and 100 μg/mL), catechin (10 μM), and quercetin (10 μM) on lipid peroxidation (LPO) and the activities of SOD, CAT, GPx, and GST were studied. Significantly higher LPO and lower activities of antioxidant enzymes were observed with the increasing concentrations of SB. Catechin or quercetin protected the erythrocytes against SB-induced toxicity only at low concentrations of SB. The presence of catechin or quercetin at 10 μM have no effect on SB-induced toxicity at high concentrations of SB (50 and 100 μg/mL). In conclusion, SB may cause oxidative stress as food additive in human erythrocytes in vitro. So, it appears that our findings provide evidence for the protection of erythrocytes from SB that could be considered for further studies. |
| format | Article |
| id | doaj-art-76cf1d3960224414b20681ace72d0b52 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-76cf1d3960224414b20681ace72d0b522025-02-03T00:59:51ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/874824874824Protective Role of Catechin and Quercetin in Sodium Benzoate-Induced Lipid Peroxidation and the Antioxidant System in Human Erythrocytes In VitroGamze Yetuk0Dilek Pandir1Hatice Bas2Department of Biology, Faculty of Arts and Science, Bozok University, 66100 Divanli Yolu/Yozgat, TurkeyDepartment of Biology, Faculty of Arts and Science, Bozok University, 66100 Divanli Yolu/Yozgat, TurkeyDepartment of Biology, Faculty of Arts and Science, Bozok University, 66100 Divanli Yolu/Yozgat, TurkeyThe aim of this study was to evaluate the protective effect of catechin and quercetin in sodium benzoate- (SB-) induced oxidative stress in human erythrocytes in vitro. For this, the effects of SB (6.25, 12.5, 25, 50, and 100 μg/mL), catechin (10 μM), and quercetin (10 μM) on lipid peroxidation (LPO) and the activities of SOD, CAT, GPx, and GST were studied. Significantly higher LPO and lower activities of antioxidant enzymes were observed with the increasing concentrations of SB. Catechin or quercetin protected the erythrocytes against SB-induced toxicity only at low concentrations of SB. The presence of catechin or quercetin at 10 μM have no effect on SB-induced toxicity at high concentrations of SB (50 and 100 μg/mL). In conclusion, SB may cause oxidative stress as food additive in human erythrocytes in vitro. So, it appears that our findings provide evidence for the protection of erythrocytes from SB that could be considered for further studies.http://dx.doi.org/10.1155/2014/874824 |
| spellingShingle | Gamze Yetuk Dilek Pandir Hatice Bas Protective Role of Catechin and Quercetin in Sodium Benzoate-Induced Lipid Peroxidation and the Antioxidant System in Human Erythrocytes In Vitro The Scientific World Journal |
| title | Protective Role of Catechin and Quercetin in Sodium Benzoate-Induced Lipid Peroxidation and the Antioxidant System in Human Erythrocytes In Vitro |
| title_full | Protective Role of Catechin and Quercetin in Sodium Benzoate-Induced Lipid Peroxidation and the Antioxidant System in Human Erythrocytes In Vitro |
| title_fullStr | Protective Role of Catechin and Quercetin in Sodium Benzoate-Induced Lipid Peroxidation and the Antioxidant System in Human Erythrocytes In Vitro |
| title_full_unstemmed | Protective Role of Catechin and Quercetin in Sodium Benzoate-Induced Lipid Peroxidation and the Antioxidant System in Human Erythrocytes In Vitro |
| title_short | Protective Role of Catechin and Quercetin in Sodium Benzoate-Induced Lipid Peroxidation and the Antioxidant System in Human Erythrocytes In Vitro |
| title_sort | protective role of catechin and quercetin in sodium benzoate induced lipid peroxidation and the antioxidant system in human erythrocytes in vitro |
| url | http://dx.doi.org/10.1155/2014/874824 |
| work_keys_str_mv | AT gamzeyetuk protectiveroleofcatechinandquercetininsodiumbenzoateinducedlipidperoxidationandtheantioxidantsysteminhumanerythrocytesinvitro AT dilekpandir protectiveroleofcatechinandquercetininsodiumbenzoateinducedlipidperoxidationandtheantioxidantsysteminhumanerythrocytesinvitro AT haticebas protectiveroleofcatechinandquercetininsodiumbenzoateinducedlipidperoxidationandtheantioxidantsysteminhumanerythrocytesinvitro |