Evaluation of how laser photostimulation at two wavelengths alters the antimicrobial potential of Streptomycetes spp
Abstract The purpose of this study was to isolate and identify additional products produced by direct laser irradiation, as well as to ascertain if laser irradiation may stimulate the synthesis of antibiotic compounds in a local Streptomycetes (Strept). Moreover, we postulate the mechanisms by which...
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Nature Portfolio
2025-08-01
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| Online Access: | https://doi.org/10.1038/s41598-025-14788-1 |
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| author | Noor Abduljabar Jadah Talib Saleh Al-Rubaye |
| author_facet | Noor Abduljabar Jadah Talib Saleh Al-Rubaye |
| author_sort | Noor Abduljabar Jadah |
| collection | DOAJ |
| description | Abstract The purpose of this study was to isolate and identify additional products produced by direct laser irradiation, as well as to ascertain if laser irradiation may stimulate the synthesis of antibiotic compounds in a local Streptomycetes (Strept). Moreover, we postulate the mechanisms by which lasers function within living bacterial cells and suggest that sequential photochemical reactions may transpire following a designated period of irradiation. Thiophene was found as one of the most significant clinical products with antibacterial and anticancer properties. To accomplish these objectives, we selected two isolates: Streptomyces thinghirensis strain S10 (Strept thin), which inherently synthesizes an antibacterial agent, and Streptomyces lienomycini strain C.P.57 (Strept.lieno), which does not generate antimicrobials. The experimental isolates were exposed to identical circumstances as the control isolates, with the exception that the inoculum underwent irradiation with a diode laser for varying durations. We initially assessed the antibacterial efficacy of the irradiation and control Strept. Gas chromatography-mass spectrometry (GC-MS) was utilized to discover antibacterial substances. Ultimately, we determined that laser irradiation caused alterations in both antimicrobial-producing and non-producing Strept.; specifically, those that produced antimicrobials ceased to do so post-irradiation, and conversely. |
| format | Article |
| id | doaj-art-76cd88a88170425c8ca15d3c663fb9f7 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-76cd88a88170425c8ca15d3c663fb9f72025-08-20T03:46:04ZengNature PortfolioScientific Reports2045-23222025-08-0115111110.1038/s41598-025-14788-1Evaluation of how laser photostimulation at two wavelengths alters the antimicrobial potential of Streptomycetes sppNoor Abduljabar Jadah0Talib Saleh Al-Rubaye1 Institute of Laser for Postgraduate Studies, University of BaghdadDepartment of Biotechnology, College of Science, University of BaghdadAbstract The purpose of this study was to isolate and identify additional products produced by direct laser irradiation, as well as to ascertain if laser irradiation may stimulate the synthesis of antibiotic compounds in a local Streptomycetes (Strept). Moreover, we postulate the mechanisms by which lasers function within living bacterial cells and suggest that sequential photochemical reactions may transpire following a designated period of irradiation. Thiophene was found as one of the most significant clinical products with antibacterial and anticancer properties. To accomplish these objectives, we selected two isolates: Streptomyces thinghirensis strain S10 (Strept thin), which inherently synthesizes an antibacterial agent, and Streptomyces lienomycini strain C.P.57 (Strept.lieno), which does not generate antimicrobials. The experimental isolates were exposed to identical circumstances as the control isolates, with the exception that the inoculum underwent irradiation with a diode laser for varying durations. We initially assessed the antibacterial efficacy of the irradiation and control Strept. Gas chromatography-mass spectrometry (GC-MS) was utilized to discover antibacterial substances. Ultimately, we determined that laser irradiation caused alterations in both antimicrobial-producing and non-producing Strept.; specifically, those that produced antimicrobials ceased to do so post-irradiation, and conversely.https://doi.org/10.1038/s41598-025-14788-1StreptomycetesLaserThiopheneGas-chromatographyAntibacterialAntitumor |
| spellingShingle | Noor Abduljabar Jadah Talib Saleh Al-Rubaye Evaluation of how laser photostimulation at two wavelengths alters the antimicrobial potential of Streptomycetes spp Scientific Reports Streptomycetes Laser Thiophene Gas-chromatography Antibacterial Antitumor |
| title | Evaluation of how laser photostimulation at two wavelengths alters the antimicrobial potential of Streptomycetes spp |
| title_full | Evaluation of how laser photostimulation at two wavelengths alters the antimicrobial potential of Streptomycetes spp |
| title_fullStr | Evaluation of how laser photostimulation at two wavelengths alters the antimicrobial potential of Streptomycetes spp |
| title_full_unstemmed | Evaluation of how laser photostimulation at two wavelengths alters the antimicrobial potential of Streptomycetes spp |
| title_short | Evaluation of how laser photostimulation at two wavelengths alters the antimicrobial potential of Streptomycetes spp |
| title_sort | evaluation of how laser photostimulation at two wavelengths alters the antimicrobial potential of streptomycetes spp |
| topic | Streptomycetes Laser Thiophene Gas-chromatography Antibacterial Antitumor |
| url | https://doi.org/10.1038/s41598-025-14788-1 |
| work_keys_str_mv | AT noorabduljabarjadah evaluationofhowlaserphotostimulationattwowavelengthsalterstheantimicrobialpotentialofstreptomycetesspp AT talibsalehalrubaye evaluationofhowlaserphotostimulationattwowavelengthsalterstheantimicrobialpotentialofstreptomycetesspp |