Methyl Red Based Test Strip for Identification of Formalin Compounds in Fish Samples

Methyl red based test strips were introduced a simple method for formalin identification. The test strip was designed based on the feasibility of the formalin oxidation reaction using hydrogen peroxide in an alkaline medium and using the methyl red indicator which is detected at a wavelength of 526...

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Main Authors: Lina Sun Haji, Choirun Nisa Anida, Caleysda Aprilianti, Himayah Izmi Fauziyyah, Diah Ayu Pitaloka, Zulfikar Zulfikar
Format: Article
Language:Indonesian
Published: Universitas Jember 2024-10-01
Series:Berkala Sainstek
Online Access:https://jurnal.unej.ac.id/index.php/BST/article/view/52156
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author Lina Sun Haji
Choirun Nisa Anida
Caleysda Aprilianti
Himayah Izmi Fauziyyah
Diah Ayu Pitaloka
Zulfikar Zulfikar
author_facet Lina Sun Haji
Choirun Nisa Anida
Caleysda Aprilianti
Himayah Izmi Fauziyyah
Diah Ayu Pitaloka
Zulfikar Zulfikar
author_sort Lina Sun Haji
collection DOAJ
description Methyl red based test strips were introduced a simple method for formalin identification. The test strip was designed based on the feasibility of the formalin oxidation reaction using hydrogen peroxide in an alkaline medium and using the methyl red indicator which is detected at a wavelength of 526 nm, with a concentration range from 1.0 - 10.0 ppm. The test strip was designed by immobilizing methyl red and NaOH in a Whatman paper matrix number 40 for 40 minutes and it has been evaluated by adding formalin samples and oxidator to the surface of the test strip. Optimum measurement conditions were obtained at 0.05 mL of 0.06M methyl red, 0.2 mL of 0.001M NaOH and 0.25 mL of 2% H2O2. The test strip has a good performance to identify formalin in the concentration range of 1.0 - 7.5 ppm which is monitored by a color change from yellow to red. The feasibility of using the test strip is also carried out by analyzing real samples through a recovery test. Test strips still can be used to identify formalin until 30 days when it stored at room temperature, and 2 days when it stored in the refrigerator.
format Article
id doaj-art-76ccf40b9e984a6ab79efa20ccdc4b20
institution DOAJ
issn 2339-0069
language Indonesian
publishDate 2024-10-01
publisher Universitas Jember
record_format Article
series Berkala Sainstek
spelling doaj-art-76ccf40b9e984a6ab79efa20ccdc4b202025-08-20T02:45:37ZindUniversitas JemberBerkala Sainstek2339-00692024-10-0112313213910.19184/bst.v12i3.5215652156Methyl Red Based Test Strip for Identification of Formalin Compounds in Fish SamplesLina Sun Haji0Choirun Nisa Anida1Caleysda Aprilianti2Himayah Izmi Fauziyyah3Diah Ayu Pitaloka4Zulfikar Zulfikar5Jurusan Kimia, FMIPA, Universitas Jember, Jalan Kalimantan 37, Jember, IndonesiaJurusan Kimia, FMIPA, Universitas Jember, Jalan Kalimantan 37, Jember, IndonesiaJurusan Kimia, FMIPA, Universitas Jember, Jalan Kalimantan 37, Jember, IndonesiaJurusan Kimia, FMIPA, Universitas Jember, Jalan Kalimantan 37, Jember, IndonesiaJurusan Kimia, FMIPA, Universitas Jember, Jalan Kalimantan 37, Jember, IndonesiaJurusan Kimia, FMIPA, Universitas Jember, Jalan Kalimantan 37, Jember, IndonesiaMethyl red based test strips were introduced a simple method for formalin identification. The test strip was designed based on the feasibility of the formalin oxidation reaction using hydrogen peroxide in an alkaline medium and using the methyl red indicator which is detected at a wavelength of 526 nm, with a concentration range from 1.0 - 10.0 ppm. The test strip was designed by immobilizing methyl red and NaOH in a Whatman paper matrix number 40 for 40 minutes and it has been evaluated by adding formalin samples and oxidator to the surface of the test strip. Optimum measurement conditions were obtained at 0.05 mL of 0.06M methyl red, 0.2 mL of 0.001M NaOH and 0.25 mL of 2% H2O2. The test strip has a good performance to identify formalin in the concentration range of 1.0 - 7.5 ppm which is monitored by a color change from yellow to red. The feasibility of using the test strip is also carried out by analyzing real samples through a recovery test. Test strips still can be used to identify formalin until 30 days when it stored at room temperature, and 2 days when it stored in the refrigerator.https://jurnal.unej.ac.id/index.php/BST/article/view/52156
spellingShingle Lina Sun Haji
Choirun Nisa Anida
Caleysda Aprilianti
Himayah Izmi Fauziyyah
Diah Ayu Pitaloka
Zulfikar Zulfikar
Methyl Red Based Test Strip for Identification of Formalin Compounds in Fish Samples
Berkala Sainstek
title Methyl Red Based Test Strip for Identification of Formalin Compounds in Fish Samples
title_full Methyl Red Based Test Strip for Identification of Formalin Compounds in Fish Samples
title_fullStr Methyl Red Based Test Strip for Identification of Formalin Compounds in Fish Samples
title_full_unstemmed Methyl Red Based Test Strip for Identification of Formalin Compounds in Fish Samples
title_short Methyl Red Based Test Strip for Identification of Formalin Compounds in Fish Samples
title_sort methyl red based test strip for identification of formalin compounds in fish samples
url https://jurnal.unej.ac.id/index.php/BST/article/view/52156
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AT choirunnisaanida methylredbasedteststripforidentificationofformalincompoundsinfishsamples
AT caleysdaaprilianti methylredbasedteststripforidentificationofformalincompoundsinfishsamples
AT himayahizmifauziyyah methylredbasedteststripforidentificationofformalincompoundsinfishsamples
AT diahayupitaloka methylredbasedteststripforidentificationofformalincompoundsinfishsamples
AT zulfikarzulfikar methylredbasedteststripforidentificationofformalincompoundsinfishsamples