Drought suppresses plant salicylic acid defence against herbivorous insects by down-regulating the expression of ICS1 via NAC transcription factor
Many herbivorous insects exhibit enhanced performance and population dynamics on drought-stressed host plants due to induced changes in plant physiology. However, the underlying mechanisms of how drought regulates plant defense against herbivorous insects are still largely unknown. In this study, we...
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| Language: | English |
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Elsevier
2025-06-01
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| Series: | Plant Stress |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X25001551 |
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| author | Chan Zhao Wen-Hao Han Yu-Die Xiong Shun-Xia Ji Hui Du Yu-Jie Chi Na Chen He Wu Shu-Sheng Liu Xiao-Wei Wang |
| author_facet | Chan Zhao Wen-Hao Han Yu-Die Xiong Shun-Xia Ji Hui Du Yu-Jie Chi Na Chen He Wu Shu-Sheng Liu Xiao-Wei Wang |
| author_sort | Chan Zhao |
| collection | DOAJ |
| description | Many herbivorous insects exhibit enhanced performance and population dynamics on drought-stressed host plants due to induced changes in plant physiology. However, the underlying mechanisms of how drought regulates plant defense against herbivorous insects are still largely unknown. In this study, we found that the survival rate and fecundity of whiteflies, notorious global pests, were significantly higher on drought-stressed tobacco. Drought stress did not affect jasmonic acid (JA) accumulation, whereas it significantly decreased the salicylic acid (SA) content by suppressing its biosynthesis. We further demonstrated that drought-induced abscisic acid (ABA) inhibited the expression of isochorismate synthase 1 (ICS1), a key enzyme in the synthesis of SA. The accumulation of ABA induced the expression of abscisic acid responsive NAC domain containing protein 19 (ANAC019), which directly bound to the promoter of NtICS1 and negatively regulated its expression. Finally, we revealed that drought and ABA could activate abscisic acid responsive elements-binding factor 2 (ABF2) transcription factor to up-regulate the expression of NtANAC019. Our study illustrates the significance of cross-talk between abiotic stress and plant-herbivore interactions and reveals the mechanisms leading to altered herbivore fitness on drought-stressed plants. |
| format | Article |
| id | doaj-art-76cb5b11f0624bb1bf86ee327ab0ad09 |
| institution | Kabale University |
| issn | 2667-064X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj-art-76cb5b11f0624bb1bf86ee327ab0ad092025-08-20T03:48:18ZengElsevierPlant Stress2667-064X2025-06-011610088710.1016/j.stress.2025.100887Drought suppresses plant salicylic acid defence against herbivorous insects by down-regulating the expression of ICS1 via NAC transcription factorChan Zhao0Wen-Hao Han1Yu-Die Xiong2Shun-Xia Ji3Hui Du4Yu-Jie Chi5Na Chen6He Wu7Shu-Sheng Liu8Xiao-Wei Wang9State Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, PR ChinaState Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, PR ChinaState Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, PR ChinaState Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, PR ChinaState Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, PR ChinaState Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, PR ChinaState Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, PR ChinaState Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, PR ChinaState Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, PR ChinaCorresponding authors.; State Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, PR ChinaMany herbivorous insects exhibit enhanced performance and population dynamics on drought-stressed host plants due to induced changes in plant physiology. However, the underlying mechanisms of how drought regulates plant defense against herbivorous insects are still largely unknown. In this study, we found that the survival rate and fecundity of whiteflies, notorious global pests, were significantly higher on drought-stressed tobacco. Drought stress did not affect jasmonic acid (JA) accumulation, whereas it significantly decreased the salicylic acid (SA) content by suppressing its biosynthesis. We further demonstrated that drought-induced abscisic acid (ABA) inhibited the expression of isochorismate synthase 1 (ICS1), a key enzyme in the synthesis of SA. The accumulation of ABA induced the expression of abscisic acid responsive NAC domain containing protein 19 (ANAC019), which directly bound to the promoter of NtICS1 and negatively regulated its expression. Finally, we revealed that drought and ABA could activate abscisic acid responsive elements-binding factor 2 (ABF2) transcription factor to up-regulate the expression of NtANAC019. Our study illustrates the significance of cross-talk between abiotic stress and plant-herbivore interactions and reveals the mechanisms leading to altered herbivore fitness on drought-stressed plants.http://www.sciencedirect.com/science/article/pii/S2667064X25001551Abscisic acidDroughtPlant-herbivoreSalicylic acidTranscription regulation |
| spellingShingle | Chan Zhao Wen-Hao Han Yu-Die Xiong Shun-Xia Ji Hui Du Yu-Jie Chi Na Chen He Wu Shu-Sheng Liu Xiao-Wei Wang Drought suppresses plant salicylic acid defence against herbivorous insects by down-regulating the expression of ICS1 via NAC transcription factor Plant Stress Abscisic acid Drought Plant-herbivore Salicylic acid Transcription regulation |
| title | Drought suppresses plant salicylic acid defence against herbivorous insects by down-regulating the expression of ICS1 via NAC transcription factor |
| title_full | Drought suppresses plant salicylic acid defence against herbivorous insects by down-regulating the expression of ICS1 via NAC transcription factor |
| title_fullStr | Drought suppresses plant salicylic acid defence against herbivorous insects by down-regulating the expression of ICS1 via NAC transcription factor |
| title_full_unstemmed | Drought suppresses plant salicylic acid defence against herbivorous insects by down-regulating the expression of ICS1 via NAC transcription factor |
| title_short | Drought suppresses plant salicylic acid defence against herbivorous insects by down-regulating the expression of ICS1 via NAC transcription factor |
| title_sort | drought suppresses plant salicylic acid defence against herbivorous insects by down regulating the expression of ics1 via nac transcription factor |
| topic | Abscisic acid Drought Plant-herbivore Salicylic acid Transcription regulation |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X25001551 |
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