How many plasmids can bacteria carry? A synthetic biology perspective
Plasmids are pinnacle tools in synthetic biology and other biotechnological applications. They serve as the simplest approach to introduce recombinant DNA, which is then transcribed into RNA that functions as is or is translated into a protein of interest. Despite their widespread utility, the quest...
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| Format: | Article |
| Language: | English |
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The Royal Society
2025-07-01
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| Series: | Open Biology |
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| Online Access: | https://royalsocietypublishing.org/doi/10.1098/rsob.240378 |
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| _version_ | 1849728188661366784 |
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| author | Cholpisit Kiattisewee |
| author_facet | Cholpisit Kiattisewee |
| author_sort | Cholpisit Kiattisewee |
| collection | DOAJ |
| description | Plasmids are pinnacle tools in synthetic biology and other biotechnological applications. They serve as the simplest approach to introduce recombinant DNA, which is then transcribed into RNA that functions as is or is translated into a protein of interest. Despite their widespread utility, the question ‘how many plasmids can be used in this bacterium?’ remains underexplored in the existing literature. In this article, I discuss the maintenance of multiple unique plasmids in bacteria through a microbial synthetic biology perspective, both in theoretical and practical aspects. I delve into the existing evidence of multi-plasmid systems, aiming to pinpoint the possible maximum number of unique plasmids a single microbe can carry. Finally, I highlight how the existing applications of multi-plasmid systems drive novel discovery and development in metabolic engineering, synthetic biology and other relevant areas in comparison to other non-plasmid strategies. |
| format | Article |
| id | doaj-art-76b11a1a9faf4ba1828dcc087a04cf9a |
| institution | DOAJ |
| issn | 2046-2441 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Open Biology |
| spelling | doaj-art-76b11a1a9faf4ba1828dcc087a04cf9a2025-08-20T03:09:38ZengThe Royal SocietyOpen Biology2046-24412025-07-0115710.1098/rsob.240378How many plasmids can bacteria carry? A synthetic biology perspectiveCholpisit Kiattisewee0Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA, USAPlasmids are pinnacle tools in synthetic biology and other biotechnological applications. They serve as the simplest approach to introduce recombinant DNA, which is then transcribed into RNA that functions as is or is translated into a protein of interest. Despite their widespread utility, the question ‘how many plasmids can be used in this bacterium?’ remains underexplored in the existing literature. In this article, I discuss the maintenance of multiple unique plasmids in bacteria through a microbial synthetic biology perspective, both in theoretical and practical aspects. I delve into the existing evidence of multi-plasmid systems, aiming to pinpoint the possible maximum number of unique plasmids a single microbe can carry. Finally, I highlight how the existing applications of multi-plasmid systems drive novel discovery and development in metabolic engineering, synthetic biology and other relevant areas in comparison to other non-plasmid strategies.https://royalsocietypublishing.org/doi/10.1098/rsob.240378plasmidsbacteriasynthetic biology |
| spellingShingle | Cholpisit Kiattisewee How many plasmids can bacteria carry? A synthetic biology perspective Open Biology plasmids bacteria synthetic biology |
| title | How many plasmids can bacteria carry? A synthetic biology perspective |
| title_full | How many plasmids can bacteria carry? A synthetic biology perspective |
| title_fullStr | How many plasmids can bacteria carry? A synthetic biology perspective |
| title_full_unstemmed | How many plasmids can bacteria carry? A synthetic biology perspective |
| title_short | How many plasmids can bacteria carry? A synthetic biology perspective |
| title_sort | how many plasmids can bacteria carry a synthetic biology perspective |
| topic | plasmids bacteria synthetic biology |
| url | https://royalsocietypublishing.org/doi/10.1098/rsob.240378 |
| work_keys_str_mv | AT cholpisitkiattisewee howmanyplasmidscanbacteriacarryasyntheticbiologyperspective |