Simultaneous Determination of Sunset Yellow FCF, Allura Red AC, Quinoline Yellow WS, and Tartrazine in Food Samples by RP-HPLC
An efficient method was developed for the simultaneous determination of Sunset Yellow FCF (E110), Allura Red AC (E129), Quinoline Yellow WS (E104), and Tartrazine (E102) in food samples by RP-HPLC. The mentioned food dyes were analyzed at room temperature for 23 min with gradient elution. Three mobi...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/6486250 |
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| author | Hakan Alp Davut Başkan Ahmet Yaşar Nurettin Yaylı Ümmühan Ocak Miraç Ocak |
| author_facet | Hakan Alp Davut Başkan Ahmet Yaşar Nurettin Yaylı Ümmühan Ocak Miraç Ocak |
| author_sort | Hakan Alp |
| collection | DOAJ |
| description | An efficient method was developed for the simultaneous determination of Sunset Yellow FCF (E110), Allura Red AC (E129), Quinoline Yellow WS (E104), and Tartrazine (E102) in food samples by RP-HPLC. The mentioned food dyes were analyzed at room temperature for 23 min with gradient elution. Three mobile phases were used for the elution, and mobile phase A was an acetate buffer (pH = 7.5, 1%), mobile phase B was acetonitrile, and mobile phase C was methanol. The flow rate was 1.0 mL min−1, and the injection volume was 20 µL. The linear ranges were 0.72–50 mg L−1, 0.24–50 mg L−1, 0.75–10 mg L−1, and 0.69–50 mg L−1 for Tartrazine, Quinoline Yellow WS, Sunset Yellow FCF, and Allura Red AC, respectively. R2 values were 0.999 for all dyes. Limits of detection were 0.24 mg L−1, 0.08 mg L−1, 0.25 mg L−1, and 0.23 mg L−1 for Tartrazine, Quinoline Yellow WS, Sunset Yellow FCF, and Allura Red AC, respectively. The relative standard deviation (RSD) of the measurements for all of the four dyes was between 0.56 and 1.65% intraday measurements. This method was successfully applied in the determination of the mentioned dyes in ice pops, gummy bears, chewing gum, and sweets candy samples. |
| format | Article |
| id | doaj-art-76ae2ab9f6f14a828866eb44abbe20e1 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-76ae2ab9f6f14a828866eb44abbe20e12025-08-20T03:35:15ZengWileyJournal of Chemistry2090-90632090-90712018-01-01201810.1155/2018/64862506486250Simultaneous Determination of Sunset Yellow FCF, Allura Red AC, Quinoline Yellow WS, and Tartrazine in Food Samples by RP-HPLCHakan Alp0Davut Başkan1Ahmet Yaşar2Nurettin Yaylı3Ümmühan Ocak4Miraç Ocak5Department of Chemistry, Faculty of Science, Karadeniz Technical University, 61080 Trabzon, TurkeyDepartment of Chemistry, Faculty of Science, Karadeniz Technical University, 61080 Trabzon, TurkeyFaculty of Pharmaceutical Sciences, Karadeniz Technical University, 61080 Trabzon, TurkeyFaculty of Pharmaceutical Sciences, Karadeniz Technical University, 61080 Trabzon, TurkeyDepartment of Chemistry, Faculty of Science, Karadeniz Technical University, 61080 Trabzon, TurkeyDepartment of Chemistry, Faculty of Science, Karadeniz Technical University, 61080 Trabzon, TurkeyAn efficient method was developed for the simultaneous determination of Sunset Yellow FCF (E110), Allura Red AC (E129), Quinoline Yellow WS (E104), and Tartrazine (E102) in food samples by RP-HPLC. The mentioned food dyes were analyzed at room temperature for 23 min with gradient elution. Three mobile phases were used for the elution, and mobile phase A was an acetate buffer (pH = 7.5, 1%), mobile phase B was acetonitrile, and mobile phase C was methanol. The flow rate was 1.0 mL min−1, and the injection volume was 20 µL. The linear ranges were 0.72–50 mg L−1, 0.24–50 mg L−1, 0.75–10 mg L−1, and 0.69–50 mg L−1 for Tartrazine, Quinoline Yellow WS, Sunset Yellow FCF, and Allura Red AC, respectively. R2 values were 0.999 for all dyes. Limits of detection were 0.24 mg L−1, 0.08 mg L−1, 0.25 mg L−1, and 0.23 mg L−1 for Tartrazine, Quinoline Yellow WS, Sunset Yellow FCF, and Allura Red AC, respectively. The relative standard deviation (RSD) of the measurements for all of the four dyes was between 0.56 and 1.65% intraday measurements. This method was successfully applied in the determination of the mentioned dyes in ice pops, gummy bears, chewing gum, and sweets candy samples.http://dx.doi.org/10.1155/2018/6486250 |
| spellingShingle | Hakan Alp Davut Başkan Ahmet Yaşar Nurettin Yaylı Ümmühan Ocak Miraç Ocak Simultaneous Determination of Sunset Yellow FCF, Allura Red AC, Quinoline Yellow WS, and Tartrazine in Food Samples by RP-HPLC Journal of Chemistry |
| title | Simultaneous Determination of Sunset Yellow FCF, Allura Red AC, Quinoline Yellow WS, and Tartrazine in Food Samples by RP-HPLC |
| title_full | Simultaneous Determination of Sunset Yellow FCF, Allura Red AC, Quinoline Yellow WS, and Tartrazine in Food Samples by RP-HPLC |
| title_fullStr | Simultaneous Determination of Sunset Yellow FCF, Allura Red AC, Quinoline Yellow WS, and Tartrazine in Food Samples by RP-HPLC |
| title_full_unstemmed | Simultaneous Determination of Sunset Yellow FCF, Allura Red AC, Quinoline Yellow WS, and Tartrazine in Food Samples by RP-HPLC |
| title_short | Simultaneous Determination of Sunset Yellow FCF, Allura Red AC, Quinoline Yellow WS, and Tartrazine in Food Samples by RP-HPLC |
| title_sort | simultaneous determination of sunset yellow fcf allura red ac quinoline yellow ws and tartrazine in food samples by rp hplc |
| url | http://dx.doi.org/10.1155/2018/6486250 |
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