In Vitro Exposure of Porcine Ovarian Follicular Cells to PCB 153 Alters Steroid Secretion But Not Their Viability—Preliminary Study
In our previous paper[1], we demonstrated that porcine follicles collected during the early stage of development are the most sensitive to the toxic action of polychlorinated biphenyl 153 (PCB 153). Follicles of this type were collected to test the effect of PCB 153 on cell steroidogenesis and viabi...
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Wiley
2002-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2002.90 |
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| author | Ewa L. Gregoraszczuk Anna K. Wjtówicz |
| author_facet | Ewa L. Gregoraszczuk Anna K. Wjtówicz |
| author_sort | Ewa L. Gregoraszczuk |
| collection | DOAJ |
| description | In our previous paper[1], we demonstrated that porcine follicles collected during the early stage of development are the most sensitive to the toxic action of polychlorinated biphenyl 153 (PCB 153). Follicles of this type were collected to test the effect of PCB 153 on cell steroidogenesis and viability. Cocultures of granulosa and theca cells were grown in M199 medium at 37ºC. Control cultures were maintained in that medium alone, while experimental ones were supplemented with PCB 153 at doses of 5, 10, 50, and 100 ng/ml. After 48, 96, and 144 h, media were collected for steroid analysis and cell viability was measured using an LDH (lactate dehydrogenase activity) cytotoxicity test. A 2-day exposure of follicular cells to all the investigated doses of PCB 153 caused a statistically significant decrease in progesterone (P4) secretion, while in doses of 50 and 100 ng/ml there was also a decrease in testosterone (T) secretion. No effect on estradiol (E2) secretion was observed. The observed decrease in P4 and T secretion, and lack of any statistically significant effect on E2 secretion by cells from small follicles exposed for 48 h to PCB, suggests that PCB 153 acts before P4 formation. Longer exposures caused an increase in P4 secretion, with a concomitant drastic decrease in T secretion and a tendency to decrease the E2 secretion, suggesting inhibition of P450 17α hydroxysteroid dehydrogenase, an enzyme that converts P4 to T. The observed PCB 153–induced increase in P4 secretion by cells collected from small antral follicles, with a concomitant decrease in E2 secretion, accounts for the induction of luteinization and, in this case, inhibition of aromatization process in the follicles. However, in all doses tested and at all times of exposure, PCB 153 had no effect on cell viability. These findings suggest different time of exposure–dependent action of PCB 153 on particular steps of steroidogenesis but not action on cell viability. These results should be considered preliminary, pending confirmation by other studies. |
| format | Article |
| id | doaj-art-76aa149a23b54bfdb2bc780df23fc210 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2002-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-76aa149a23b54bfdb2bc780df23fc2102025-08-20T03:35:15ZengWileyThe Scientific World Journal1537-744X2002-01-01226126710.1100/tsw.2002.90In Vitro Exposure of Porcine Ovarian Follicular Cells to PCB 153 Alters Steroid Secretion But Not Their Viability—Preliminary StudyEwa L. Gregoraszczuk0Anna K. Wjtówicz1Laboratory of Physiology and Toxicology of Reproduction, Department of Physiology, Institute of Zoology, Jagiellonian University, Ingardena 6, 30-060 Kraków, PolandLaboratory of Physiology and Toxicology of Reproduction, Department of Physiology, Institute of Zoology, Jagiellonian University, Ingardena 6, 30-060 Kraków, PolandIn our previous paper[1], we demonstrated that porcine follicles collected during the early stage of development are the most sensitive to the toxic action of polychlorinated biphenyl 153 (PCB 153). Follicles of this type were collected to test the effect of PCB 153 on cell steroidogenesis and viability. Cocultures of granulosa and theca cells were grown in M199 medium at 37ºC. Control cultures were maintained in that medium alone, while experimental ones were supplemented with PCB 153 at doses of 5, 10, 50, and 100 ng/ml. After 48, 96, and 144 h, media were collected for steroid analysis and cell viability was measured using an LDH (lactate dehydrogenase activity) cytotoxicity test. A 2-day exposure of follicular cells to all the investigated doses of PCB 153 caused a statistically significant decrease in progesterone (P4) secretion, while in doses of 50 and 100 ng/ml there was also a decrease in testosterone (T) secretion. No effect on estradiol (E2) secretion was observed. The observed decrease in P4 and T secretion, and lack of any statistically significant effect on E2 secretion by cells from small follicles exposed for 48 h to PCB, suggests that PCB 153 acts before P4 formation. Longer exposures caused an increase in P4 secretion, with a concomitant drastic decrease in T secretion and a tendency to decrease the E2 secretion, suggesting inhibition of P450 17α hydroxysteroid dehydrogenase, an enzyme that converts P4 to T. The observed PCB 153–induced increase in P4 secretion by cells collected from small antral follicles, with a concomitant decrease in E2 secretion, accounts for the induction of luteinization and, in this case, inhibition of aromatization process in the follicles. However, in all doses tested and at all times of exposure, PCB 153 had no effect on cell viability. These findings suggest different time of exposure–dependent action of PCB 153 on particular steps of steroidogenesis but not action on cell viability. These results should be considered preliminary, pending confirmation by other studies.http://dx.doi.org/10.1100/tsw.2002.90 |
| spellingShingle | Ewa L. Gregoraszczuk Anna K. Wjtówicz In Vitro Exposure of Porcine Ovarian Follicular Cells to PCB 153 Alters Steroid Secretion But Not Their Viability—Preliminary Study The Scientific World Journal |
| title | In Vitro Exposure of Porcine Ovarian Follicular Cells to PCB 153 Alters Steroid Secretion But Not Their Viability—Preliminary Study |
| title_full | In Vitro Exposure of Porcine Ovarian Follicular Cells to PCB 153 Alters Steroid Secretion But Not Their Viability—Preliminary Study |
| title_fullStr | In Vitro Exposure of Porcine Ovarian Follicular Cells to PCB 153 Alters Steroid Secretion But Not Their Viability—Preliminary Study |
| title_full_unstemmed | In Vitro Exposure of Porcine Ovarian Follicular Cells to PCB 153 Alters Steroid Secretion But Not Their Viability—Preliminary Study |
| title_short | In Vitro Exposure of Porcine Ovarian Follicular Cells to PCB 153 Alters Steroid Secretion But Not Their Viability—Preliminary Study |
| title_sort | in vitro exposure of porcine ovarian follicular cells to pcb 153 alters steroid secretion but not their viability preliminary study |
| url | http://dx.doi.org/10.1100/tsw.2002.90 |
| work_keys_str_mv | AT ewalgregoraszczuk invitroexposureofporcineovarianfollicularcellstopcb153alterssteroidsecretionbutnottheirviabilitypreliminarystudy AT annakwjtowicz invitroexposureofporcineovarianfollicularcellstopcb153alterssteroidsecretionbutnottheirviabilitypreliminarystudy |