Cutibacterium acnes of single locus sequence type clade C strains exhibit low susceptibility to benzoyl peroxide

Background: Cutibacterium acnes plays an important role in acne pathogenesis. Benzoyl peroxide (BPO) exhibits antimicrobial activity against C. acnes. We previously found that no clade C strains, as determined by single locus sequence typing (SLST), were found before the introduction of BPO in Japan...

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Main Authors: Natsumi Sakaguchi, Shoji Seyama, Ryota Oba, Nobukazu Hayashi, Hidemasa Nakaminami
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Journal of Global Antimicrobial Resistance
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Online Access:http://www.sciencedirect.com/science/article/pii/S221371652500102X
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author Natsumi Sakaguchi
Shoji Seyama
Ryota Oba
Nobukazu Hayashi
Hidemasa Nakaminami
author_facet Natsumi Sakaguchi
Shoji Seyama
Ryota Oba
Nobukazu Hayashi
Hidemasa Nakaminami
author_sort Natsumi Sakaguchi
collection DOAJ
description Background: Cutibacterium acnes plays an important role in acne pathogenesis. Benzoyl peroxide (BPO) exhibits antimicrobial activity against C. acnes. We previously found that no clade C strains, as determined by single locus sequence typing (SLST), were found before the introduction of BPO in Japan. However, the prevalence of clade C strains increased after its introduction. In this study, we evaluated the susceptibility of C. acnes strains, belonging to different SLST clades, to BPO. Methods: Forty clinical C. acnes isolates from four distinct SLST clades obtained from acne lesions were analyzed. The susceptibility of C. acnes isolates to BPO was evaluated using a time-kill kinetic assay. Results: C. acnes clade C strains were less susceptible to BPO. In addition, the bactericidal effect of BPO was time-dependent across all clade strains. Conclusions: An increase in C. acnes clade C strains may diminish the efficacy of acne treatment. Therefore, the trends in the prevalence of C. acnes clade C strains should be monitored.
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publishDate 2025-06-01
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series Journal of Global Antimicrobial Resistance
spelling doaj-art-769d0e4661cf4e22aff1e5f99f2f010e2025-08-20T02:35:06ZengElsevierJournal of Global Antimicrobial Resistance2213-71652025-06-014330630810.1016/j.jgar.2025.04.030Cutibacterium acnes of single locus sequence type clade C strains exhibit low susceptibility to benzoyl peroxideNatsumi Sakaguchi0Shoji Seyama1Ryota Oba2Nobukazu Hayashi3Hidemasa Nakaminami4Department of Clinical Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, JapanDepartment of Clinical Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, JapanDepartment of Clinical Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, JapanDepartment of Dermatology, Toranomon Hospital, Minato-ku, Tokyo, JapanDepartment of Clinical Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan; Corresponding author. Mailing address: Hidemasa Nakaminami, Ph.D., Department of Clinical Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan.Background: Cutibacterium acnes plays an important role in acne pathogenesis. Benzoyl peroxide (BPO) exhibits antimicrobial activity against C. acnes. We previously found that no clade C strains, as determined by single locus sequence typing (SLST), were found before the introduction of BPO in Japan. However, the prevalence of clade C strains increased after its introduction. In this study, we evaluated the susceptibility of C. acnes strains, belonging to different SLST clades, to BPO. Methods: Forty clinical C. acnes isolates from four distinct SLST clades obtained from acne lesions were analyzed. The susceptibility of C. acnes isolates to BPO was evaluated using a time-kill kinetic assay. Results: C. acnes clade C strains were less susceptible to BPO. In addition, the bactericidal effect of BPO was time-dependent across all clade strains. Conclusions: An increase in C. acnes clade C strains may diminish the efficacy of acne treatment. Therefore, the trends in the prevalence of C. acnes clade C strains should be monitored.http://www.sciencedirect.com/science/article/pii/S221371652500102XCutibacterium acnesAcne vulgarisBenzoyl peroxideClade C
spellingShingle Natsumi Sakaguchi
Shoji Seyama
Ryota Oba
Nobukazu Hayashi
Hidemasa Nakaminami
Cutibacterium acnes of single locus sequence type clade C strains exhibit low susceptibility to benzoyl peroxide
Journal of Global Antimicrobial Resistance
Cutibacterium acnes
Acne vulgaris
Benzoyl peroxide
Clade C
title Cutibacterium acnes of single locus sequence type clade C strains exhibit low susceptibility to benzoyl peroxide
title_full Cutibacterium acnes of single locus sequence type clade C strains exhibit low susceptibility to benzoyl peroxide
title_fullStr Cutibacterium acnes of single locus sequence type clade C strains exhibit low susceptibility to benzoyl peroxide
title_full_unstemmed Cutibacterium acnes of single locus sequence type clade C strains exhibit low susceptibility to benzoyl peroxide
title_short Cutibacterium acnes of single locus sequence type clade C strains exhibit low susceptibility to benzoyl peroxide
title_sort cutibacterium acnes of single locus sequence type clade c strains exhibit low susceptibility to benzoyl peroxide
topic Cutibacterium acnes
Acne vulgaris
Benzoyl peroxide
Clade C
url http://www.sciencedirect.com/science/article/pii/S221371652500102X
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