Multimodal Coherent Anti-Stokes Raman Scattering Microscopy With a Supercontinuum All-Fiber Laser

We demonstrate a compact supercontinuum (SC) all-fiber laser source for multimodal coherent anti-Stokes Raman scattering (CARS) microscopy based on an Er-fiber seed. It can be used in both high-speed narrowband CARS microscopy and broadband CARS spectroscopy at tens of milliseconds per pixel, by sim...

Full description

Saved in:
Bibliographic Details
Main Authors: Yan Li, Xiaosheng Xiao, Jingjing Guo, Kangjun Zhao, Shunyu Yao, Lili Gui, Changxi Yang
Format: Article
Language:English
Published: IEEE 2019-01-01
Series:IEEE Photonics Journal
Subjects:
Online Access:https://ieeexplore.ieee.org/document/8648457/
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849422960404725760
author Yan Li
Xiaosheng Xiao
Jingjing Guo
Kangjun Zhao
Shunyu Yao
Lili Gui
Changxi Yang
author_facet Yan Li
Xiaosheng Xiao
Jingjing Guo
Kangjun Zhao
Shunyu Yao
Lili Gui
Changxi Yang
author_sort Yan Li
collection DOAJ
description We demonstrate a compact supercontinuum (SC) all-fiber laser source for multimodal coherent anti-Stokes Raman scattering (CARS) microscopy based on an Er-fiber seed. It can be used in both high-speed narrowband CARS microscopy and broadband CARS spectroscopy at tens of milliseconds per pixel, by simply switching without realignment. The common pump pulses, generated from the SC in a highly nonlinear fiber (HNLF), are obtained after spectral filtering. The central wavelength of pump pulses is 1067.2&#x00A0;nm with a bandwidth of 0.8&#x00A0;nm, which provides a high-spectral resolution (&lt;10&#x00A0;cm<sup>&#x2013;1</sup>). By combining with the narrowband Stokes beam, which is directly obtained from the Er-fiber oscillator around &#x223C;1550&#x00A0;nm, we can achieve a narrowband CARS microscopy in frequency shifts of &#x223C;2700 to &#x223C;3200&#x00A0;cm<sup>&#x2013;1</sup>. The SC Stokes beam in a broadband CARS spectroscopy ranging from 1150 to beyond 1700&#x00A0;nm is generated in an HNLF, with vibrational spectra over a bandwidth of more than 2800&#x00A0;cm<sup>&#x2013;1</sup>. We also analyzed the influences of different HNLFs on the SC generation both in simulation and experiment. The capability of our system in a multimodal CARS microscopy is demonstrated by the CARS detection of polystyrene and pork.
format Article
id doaj-art-7697cf5b0e2f4b038dc01ba5870e04cc
institution Kabale University
issn 1943-0655
language English
publishDate 2019-01-01
publisher IEEE
record_format Article
series IEEE Photonics Journal
spelling doaj-art-7697cf5b0e2f4b038dc01ba5870e04cc2025-08-20T03:30:51ZengIEEEIEEE Photonics Journal1943-06552019-01-011121810.1109/JPHOT.2019.28982228648457Multimodal Coherent Anti-Stokes Raman Scattering Microscopy With a Supercontinuum All-Fiber LaserYan Li0Xiaosheng Xiao1https://orcid.org/0000-0002-9368-9956Jingjing Guo2Kangjun Zhao3Shunyu Yao4Lili Gui5https://orcid.org/0000-0002-3710-6330Changxi Yang6https://orcid.org/0000-0002-9972-6965State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments, Tsinghua University, Beijing, ChinaState Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments, Tsinghua University, Beijing, ChinaState Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments, Tsinghua University, Beijing, ChinaState Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments, Tsinghua University, Beijing, ChinaState Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments, Tsinghua University, Beijing, China4th Physics Institute and Research Center SCoPE, University of Stuttgart, Stuttgart, GermanyState Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments, Tsinghua University, Beijing, ChinaWe demonstrate a compact supercontinuum (SC) all-fiber laser source for multimodal coherent anti-Stokes Raman scattering (CARS) microscopy based on an Er-fiber seed. It can be used in both high-speed narrowband CARS microscopy and broadband CARS spectroscopy at tens of milliseconds per pixel, by simply switching without realignment. The common pump pulses, generated from the SC in a highly nonlinear fiber (HNLF), are obtained after spectral filtering. The central wavelength of pump pulses is 1067.2&#x00A0;nm with a bandwidth of 0.8&#x00A0;nm, which provides a high-spectral resolution (&lt;10&#x00A0;cm<sup>&#x2013;1</sup>). By combining with the narrowband Stokes beam, which is directly obtained from the Er-fiber oscillator around &#x223C;1550&#x00A0;nm, we can achieve a narrowband CARS microscopy in frequency shifts of &#x223C;2700 to &#x223C;3200&#x00A0;cm<sup>&#x2013;1</sup>. The SC Stokes beam in a broadband CARS spectroscopy ranging from 1150 to beyond 1700&#x00A0;nm is generated in an HNLF, with vibrational spectra over a bandwidth of more than 2800&#x00A0;cm<sup>&#x2013;1</sup>. We also analyzed the influences of different HNLFs on the SC generation both in simulation and experiment. The capability of our system in a multimodal CARS microscopy is demonstrated by the CARS detection of polystyrene and pork.https://ieeexplore.ieee.org/document/8648457/Fiber lasersnonlinear opticssupercontinuum generationRaman scattering
spellingShingle Yan Li
Xiaosheng Xiao
Jingjing Guo
Kangjun Zhao
Shunyu Yao
Lili Gui
Changxi Yang
Multimodal Coherent Anti-Stokes Raman Scattering Microscopy With a Supercontinuum All-Fiber Laser
IEEE Photonics Journal
Fiber lasers
nonlinear optics
supercontinuum generation
Raman scattering
title Multimodal Coherent Anti-Stokes Raman Scattering Microscopy With a Supercontinuum All-Fiber Laser
title_full Multimodal Coherent Anti-Stokes Raman Scattering Microscopy With a Supercontinuum All-Fiber Laser
title_fullStr Multimodal Coherent Anti-Stokes Raman Scattering Microscopy With a Supercontinuum All-Fiber Laser
title_full_unstemmed Multimodal Coherent Anti-Stokes Raman Scattering Microscopy With a Supercontinuum All-Fiber Laser
title_short Multimodal Coherent Anti-Stokes Raman Scattering Microscopy With a Supercontinuum All-Fiber Laser
title_sort multimodal coherent anti stokes raman scattering microscopy with a supercontinuum all fiber laser
topic Fiber lasers
nonlinear optics
supercontinuum generation
Raman scattering
url https://ieeexplore.ieee.org/document/8648457/
work_keys_str_mv AT yanli multimodalcoherentantistokesramanscatteringmicroscopywithasupercontinuumallfiberlaser
AT xiaoshengxiao multimodalcoherentantistokesramanscatteringmicroscopywithasupercontinuumallfiberlaser
AT jingjingguo multimodalcoherentantistokesramanscatteringmicroscopywithasupercontinuumallfiberlaser
AT kangjunzhao multimodalcoherentantistokesramanscatteringmicroscopywithasupercontinuumallfiberlaser
AT shunyuyao multimodalcoherentantistokesramanscatteringmicroscopywithasupercontinuumallfiberlaser
AT liligui multimodalcoherentantistokesramanscatteringmicroscopywithasupercontinuumallfiberlaser
AT changxiyang multimodalcoherentantistokesramanscatteringmicroscopywithasupercontinuumallfiberlaser