Multimodal Coherent Anti-Stokes Raman Scattering Microscopy With a Supercontinuum All-Fiber Laser
We demonstrate a compact supercontinuum (SC) all-fiber laser source for multimodal coherent anti-Stokes Raman scattering (CARS) microscopy based on an Er-fiber seed. It can be used in both high-speed narrowband CARS microscopy and broadband CARS spectroscopy at tens of milliseconds per pixel, by sim...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
IEEE
2019-01-01
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| Series: | IEEE Photonics Journal |
| Subjects: | |
| Online Access: | https://ieeexplore.ieee.org/document/8648457/ |
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| Summary: | We demonstrate a compact supercontinuum (SC) all-fiber laser source for multimodal coherent anti-Stokes Raman scattering (CARS) microscopy based on an Er-fiber seed. It can be used in both high-speed narrowband CARS microscopy and broadband CARS spectroscopy at tens of milliseconds per pixel, by simply switching without realignment. The common pump pulses, generated from the SC in a highly nonlinear fiber (HNLF), are obtained after spectral filtering. The central wavelength of pump pulses is 1067.2 nm with a bandwidth of 0.8 nm, which provides a high-spectral resolution (<10 cm<sup>–1</sup>). By combining with the narrowband Stokes beam, which is directly obtained from the Er-fiber oscillator around ∼1550 nm, we can achieve a narrowband CARS microscopy in frequency shifts of ∼2700 to ∼3200 cm<sup>–1</sup>. The SC Stokes beam in a broadband CARS spectroscopy ranging from 1150 to beyond 1700 nm is generated in an HNLF, with vibrational spectra over a bandwidth of more than 2800 cm<sup>–1</sup>. We also analyzed the influences of different HNLFs on the SC generation both in simulation and experiment. The capability of our system in a multimodal CARS microscopy is demonstrated by the CARS detection of polystyrene and pork. |
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| ISSN: | 1943-0655 |