Antioxidant ativity of dichloromethane extract from cocoa pod husk (Theobroma cacao L.)
Theobroma cacao L. is a plant that is reported to have strong antioxidant potential. A study was carried out to determine the antioxidant activity of dichloromethane extract of cocoa pod husk using 1,1-diphenyl-2-picrylhydrazyl (DPPH). The cocoa pod husk was macerated using n-hexane, ammonia-dichlor...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universitas Syiah Kuala, Faculty of Mathematics and Natural Science
2024-06-01
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| Series: | Jurnal Natural |
| Subjects: | |
| Online Access: | https://jurnal.usk.ac.id/natural/article/view/36174 |
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| Summary: | Theobroma cacao L. is a plant that is reported to have strong antioxidant potential. A study was carried out to determine the antioxidant activity of dichloromethane extract of cocoa pod husk using 1,1-diphenyl-2-picrylhydrazyl (DPPH). The cocoa pod husk was macerated using n-hexane, ammonia-dichloromethane, and methanol. The TCD extract of cocoa pod husk showed the lowest IC50 value of 14.59 ± 0.54 ppm based on the DPPH inhibition assay. The dichloromethane extract was isolated by gravity column chromatography with Silica gel G60 as the stationary phase and n-hexane: ethyl acetate as the mobile phase (gradient elution system). Fractions with identical staining patterns were combined into six fractions (TCD 1-TCD 6). The TCD 6 showed the lowest IC50 value of 127.05 ± 0.54 ppm according to the DPPH inhibition assay. Based on the TLC results, TCD 4 was further isolated and four fractions (TCD 4.1 to TCD 4.4) were obtained. The TCD 4.4 had the lowest IC50 value (38.66 ± 0.96 ppm). The TCD extract showed the presence of the dominant compound, γ-sitosterol, as determined by GC-MS analysis. |
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| ISSN: | 1411-8513 2541-4062 |