GROWTH CAPACITY OF BACILLUS POTENTIAL STARTER STRAINS ISOLATED FROM COCOA BEANS FERMENTATION UNDER CULTURE STRESS CONDITIONS
The study investigates the growth capacity of Bacillus with pectinolytic activity, acidifying and citrate metabolism capacities under culture stress conditions. Spontaneous heaps fermentation was conducted from cocoa of six producing regions of Côte d’Ivoire. Bacillus isolated using plate agar on nu...
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Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
2017-06-01
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| Series: | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
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| Online Access: | http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201702&vol=2&aid=4563 |
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| author | WILFRIED YAO BERNADETTE G. GOUALIE HONORE G. OUATTARA SEBASTIEN NIAMKE |
| author_facet | WILFRIED YAO BERNADETTE G. GOUALIE HONORE G. OUATTARA SEBASTIEN NIAMKE |
| author_sort | WILFRIED YAO |
| collection | DOAJ |
| description | The study investigates the growth capacity of Bacillus with pectinolytic activity, acidifying and citrate metabolism capacities under culture stress conditions. Spontaneous heaps fermentation was conducted from cocoa of six producing regions of Côte d’Ivoire. Bacillus isolated using plate agar on nutrient medium were analyzed for pectinolytic enzymes production, citric acid breakdown, acidification and growth capacity under different stress conditions. A total of 970 Bacillus strains were isolated and 44.53 % of them produced pectinolytic activity. Among pectinolytic strains, 163 (37.73 %) exhibited acidifying and citrate metabolism capacity. Five (5) of these strains exhibited a strong thermotolerance at 50 ºC with the optimal growth at 35 ºC and a good capacity to grow at pH 4 to pH 8. Moreover, sugar concentrations ranged 5 to 25 % showed low effect on all tested strains growth with a maximum growth at 5 % fructose and sucrose concentration and at 15% glucose concentration. However, ethanol stress conditions (up to 8 %) repress strongly growth capacity of the strains analyzed. This study indicates that Bacillus strains involved in Ivorian cocoa fermentation possess some properties essential for a well-fermented cocoa. Therefore, these results show that Bacillus studied should be potential candidate as starter for cocoa beans fermentation control. |
| format | Article |
| id | doaj-art-76761a33446c44eb8aa5be93919337eb |
| institution | DOAJ |
| issn | 1582-540X 1582-540X |
| language | English |
| publishDate | 2017-06-01 |
| publisher | Alma Mater Publishing House "Vasile Alecsandri" University of Bacau |
| record_format | Article |
| series | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
| spelling | doaj-art-76761a33446c44eb8aa5be93919337eb2025-08-20T03:19:14ZengAlma Mater Publishing House "Vasile Alecsandri" University of BacauScientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry1582-540X1582-540X2017-06-01182201211GROWTH CAPACITY OF BACILLUS POTENTIAL STARTER STRAINS ISOLATED FROM COCOA BEANS FERMENTATION UNDER CULTURE STRESS CONDITIONSWILFRIED YAO0BERNADETTE G. GOUALIE 1HONORE G. OUATTARA2SEBASTIEN NIAMKE3Université Félix Houphouët-Boigny, UFR Biosciences, Laboratoire de Biotechnologies, 22 BP 582 Abidjan 22, Abidjan, Côte d’Ivoire Université Félix Houphouët-Boigny, UFR Biosciences, Laboratoire de Biotechnologies, 22 BP 582 Abidjan 22, Abidjan, Côte d’Ivoire Université Félix Houphouët-Boigny, UFR Biosciences, Laboratoire de Biotechnologies, 22 BP 582 Abidjan 22, Abidjan, Côte d’Ivoire Université Félix Houphouët-Boigny, UFR Biosciences, Laboratoire de Biotechnologies, 22 BP 582 Abidjan 22, Abidjan, Côte d’Ivoire The study investigates the growth capacity of Bacillus with pectinolytic activity, acidifying and citrate metabolism capacities under culture stress conditions. Spontaneous heaps fermentation was conducted from cocoa of six producing regions of Côte d’Ivoire. Bacillus isolated using plate agar on nutrient medium were analyzed for pectinolytic enzymes production, citric acid breakdown, acidification and growth capacity under different stress conditions. A total of 970 Bacillus strains were isolated and 44.53 % of them produced pectinolytic activity. Among pectinolytic strains, 163 (37.73 %) exhibited acidifying and citrate metabolism capacity. Five (5) of these strains exhibited a strong thermotolerance at 50 ºC with the optimal growth at 35 ºC and a good capacity to grow at pH 4 to pH 8. Moreover, sugar concentrations ranged 5 to 25 % showed low effect on all tested strains growth with a maximum growth at 5 % fructose and sucrose concentration and at 15% glucose concentration. However, ethanol stress conditions (up to 8 %) repress strongly growth capacity of the strains analyzed. This study indicates that Bacillus strains involved in Ivorian cocoa fermentation possess some properties essential for a well-fermented cocoa. Therefore, these results show that Bacillus studied should be potential candidate as starter for cocoa beans fermentation control.http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201702&vol=2&aid=4563Bacilluscocoa beans fermentationstress conditionstechno functional properties |
| spellingShingle | WILFRIED YAO BERNADETTE G. GOUALIE HONORE G. OUATTARA SEBASTIEN NIAMKE GROWTH CAPACITY OF BACILLUS POTENTIAL STARTER STRAINS ISOLATED FROM COCOA BEANS FERMENTATION UNDER CULTURE STRESS CONDITIONS Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry Bacillus cocoa beans fermentation stress conditions techno functional properties |
| title | GROWTH CAPACITY OF BACILLUS POTENTIAL STARTER STRAINS ISOLATED FROM COCOA BEANS FERMENTATION UNDER CULTURE STRESS CONDITIONS |
| title_full | GROWTH CAPACITY OF BACILLUS POTENTIAL STARTER STRAINS ISOLATED FROM COCOA BEANS FERMENTATION UNDER CULTURE STRESS CONDITIONS |
| title_fullStr | GROWTH CAPACITY OF BACILLUS POTENTIAL STARTER STRAINS ISOLATED FROM COCOA BEANS FERMENTATION UNDER CULTURE STRESS CONDITIONS |
| title_full_unstemmed | GROWTH CAPACITY OF BACILLUS POTENTIAL STARTER STRAINS ISOLATED FROM COCOA BEANS FERMENTATION UNDER CULTURE STRESS CONDITIONS |
| title_short | GROWTH CAPACITY OF BACILLUS POTENTIAL STARTER STRAINS ISOLATED FROM COCOA BEANS FERMENTATION UNDER CULTURE STRESS CONDITIONS |
| title_sort | growth capacity of bacillus potential starter strains isolated from cocoa beans fermentation under culture stress conditions |
| topic | Bacillus cocoa beans fermentation stress conditions techno functional properties |
| url | http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201702&vol=2&aid=4563 |
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