The immunology of corneal limbal stem cells: the up-to-date approach to stem cell transplantation
Limbal epithelial stem cells (LSC, LESC) are multipotent cells used as regenerative treatment of the cornea in patients with limbal epithelial stem cell deficiency (LSCD, LESCD). There are different types of stem cell grafting including cultivated limbal epithelial transplantation (CET) and simple...
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2023-09-01
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| Series: | Central European Journal of Immunology |
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| Online Access: | https://www.termedia.pl/The-immunology-of-corneal-limbal-stem-cells-the-up-to-date-approach-to-stem-cell-transplantation,10,51642,1,1.html |
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| author | Katarzyna Samelska Magdalena Kupis Katarzyna Szymanek Justyna Izdebska Anna Zaleska-Żmijewska Piotr Skopiński |
| author_facet | Katarzyna Samelska Magdalena Kupis Katarzyna Szymanek Justyna Izdebska Anna Zaleska-Żmijewska Piotr Skopiński |
| author_sort | Katarzyna Samelska |
| collection | DOAJ |
| description | Limbal epithelial stem cells (LSC, LESC) are multipotent cells used as regenerative treatment of the cornea in patients with limbal epithelial stem cell deficiency (LSCD, LESCD).
There are different types of stem cell grafting including cultivated limbal epithelial transplantation (CET) and simple limbal epithelial transplantation (SLET). The outcomes of the techniques have been assessed as similar, with differences in the sample size required during the procedures.
The most important culture components for stem cell cultivation include 3T3 murine fibroblasts, human amniotic membrane (HAM), fibrin gel, and culture medium. The culture medium may be enriched with serum or not; however, xenobiotic-free materials are preferred because of the low risk of pathogen transmission.
Multiple studies have defined molecules important for maintaining the function of LSC including C/EBPδ, Bmi-1, p63α, interleukins (IL-6), epithelial structural proteins – keratins, and antibodies against epidermal growth factor receptor (EGFR). The cell phenotype of LSC has been described with factors of transplantation success rate such as a high percentage of p63 positive cells.
The article emphasizes the role of recipient tissue preparation, modern cultivation techniques and pathophysiological processes in LSC transplantation effectiveness. |
| format | Article |
| id | doaj-art-76743b6ed488460c8692aa26a3ad64ea |
| institution | OA Journals |
| issn | 1426-3912 1644-4124 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Central European Journal of Immunology |
| spelling | doaj-art-76743b6ed488460c8692aa26a3ad64ea2025-08-20T02:09:38ZengTermedia Publishing HouseCentral European Journal of Immunology1426-39121644-41242023-09-0148324525010.5114/ceji.2023.13203351642The immunology of corneal limbal stem cells: the up-to-date approach to stem cell transplantationKatarzyna SamelskaMagdalena KupisKatarzyna SzymanekJustyna IzdebskaAnna Zaleska-ŻmijewskaPiotr SkopińskiLimbal epithelial stem cells (LSC, LESC) are multipotent cells used as regenerative treatment of the cornea in patients with limbal epithelial stem cell deficiency (LSCD, LESCD). There are different types of stem cell grafting including cultivated limbal epithelial transplantation (CET) and simple limbal epithelial transplantation (SLET). The outcomes of the techniques have been assessed as similar, with differences in the sample size required during the procedures. The most important culture components for stem cell cultivation include 3T3 murine fibroblasts, human amniotic membrane (HAM), fibrin gel, and culture medium. The culture medium may be enriched with serum or not; however, xenobiotic-free materials are preferred because of the low risk of pathogen transmission. Multiple studies have defined molecules important for maintaining the function of LSC including C/EBPδ, Bmi-1, p63α, interleukins (IL-6), epithelial structural proteins – keratins, and antibodies against epidermal growth factor receptor (EGFR). The cell phenotype of LSC has been described with factors of transplantation success rate such as a high percentage of p63 positive cells. The article emphasizes the role of recipient tissue preparation, modern cultivation techniques and pathophysiological processes in LSC transplantation effectiveness.https://www.termedia.pl/The-immunology-of-corneal-limbal-stem-cells-the-up-to-date-approach-to-stem-cell-transplantation,10,51642,1,1.htmlclau clet limbal epithelial stem cells limbal epithelial transplantation slet stem cell deficiency stem cell cultivation |
| spellingShingle | Katarzyna Samelska Magdalena Kupis Katarzyna Szymanek Justyna Izdebska Anna Zaleska-Żmijewska Piotr Skopiński The immunology of corneal limbal stem cells: the up-to-date approach to stem cell transplantation Central European Journal of Immunology clau clet limbal epithelial stem cells limbal epithelial transplantation slet stem cell deficiency stem cell cultivation |
| title | The immunology of corneal limbal stem cells: the up-to-date approach to stem cell transplantation |
| title_full | The immunology of corneal limbal stem cells: the up-to-date approach to stem cell transplantation |
| title_fullStr | The immunology of corneal limbal stem cells: the up-to-date approach to stem cell transplantation |
| title_full_unstemmed | The immunology of corneal limbal stem cells: the up-to-date approach to stem cell transplantation |
| title_short | The immunology of corneal limbal stem cells: the up-to-date approach to stem cell transplantation |
| title_sort | immunology of corneal limbal stem cells the up to date approach to stem cell transplantation |
| topic | clau clet limbal epithelial stem cells limbal epithelial transplantation slet stem cell deficiency stem cell cultivation |
| url | https://www.termedia.pl/The-immunology-of-corneal-limbal-stem-cells-the-up-to-date-approach-to-stem-cell-transplantation,10,51642,1,1.html |
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