Developing a Novel and Convenient Model for Investigating Sweat Gland Morphogenesis from Epidermal Stem Cells
Sweat glands developed from the embryonic epidermis. To elucidate the underlying mechanisms of morphogenesis, a reliable in vitro test system for bioactive screening must be developed. Here, we described a novel and convenient model by coculturing embryonic tissue and epidermal stem cells (ESCs) usi...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/4254759 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558250808049664 |
|---|---|
| author | Tian Hu Yongde Xu Bin Yao Xiaobing Fu Sha Huang |
| author_facet | Tian Hu Yongde Xu Bin Yao Xiaobing Fu Sha Huang |
| author_sort | Tian Hu |
| collection | DOAJ |
| description | Sweat glands developed from the embryonic epidermis. To elucidate the underlying mechanisms of morphogenesis, a reliable in vitro test system for bioactive screening must be developed. Here, we described a novel and convenient model by coculturing embryonic tissue and epidermal stem cells (ESCs) using Transwell insert for evaluating the effects of soluble morphogens on sweat gland morphogenesis in vitro. Using this coculture system, morphological alteration, histological features, and specific markers were observed. Initial experiments revealed that ESCs cocultured with embryonic paw pad (EPP) tissue demonstrated glandular structure and cytokeratin 8 (K8) and cytokeratin 18 (K18) positive, while ESCs cocultured with embryonic dorsal skin demonstrated “sea snail” structure and K8, K18 negative. Moreover, bone morphogenetic protein 4 (BMP4) and epidermal growth factor (EGF) concentrations were detected in the medium of the EPP group. BMP receptor inhibitor could effectively block the ESC differentiation to sweat glands, while EGF receptor blocker did not show the effect. Our results showed clear benefits of this novel and convenient model in terms of in vitro-in vivo correlation. It was an appropriate alternative for screening of potential bioactives regulating the sweat gland morphogenesis mechanism. |
| format | Article |
| id | doaj-art-766ccdb159174e1b93cc70769fc4c78e |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-766ccdb159174e1b93cc70769fc4c78e2025-02-03T01:32:51ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/42547594254759Developing a Novel and Convenient Model for Investigating Sweat Gland Morphogenesis from Epidermal Stem CellsTian Hu0Yongde Xu1Bin Yao2Xiaobing Fu3Sha Huang4School of Medicine, Nankai University, Tianjin 300052, ChinaDepartment of Urology, Beijing Friendship Hospital, The Capital University of Medical Sciences, Beijing 100050, ChinaWound Healing and Cell Biology Laboratory, Institute of Basic Medical Sciences, General Hospital of PLA, Beijing 100853, ChinaSchool of Medicine, Nankai University, Tianjin 300052, ChinaWound Healing and Cell Biology Laboratory, Institute of Basic Medical Sciences, General Hospital of PLA, Beijing 100853, ChinaSweat glands developed from the embryonic epidermis. To elucidate the underlying mechanisms of morphogenesis, a reliable in vitro test system for bioactive screening must be developed. Here, we described a novel and convenient model by coculturing embryonic tissue and epidermal stem cells (ESCs) using Transwell insert for evaluating the effects of soluble morphogens on sweat gland morphogenesis in vitro. Using this coculture system, morphological alteration, histological features, and specific markers were observed. Initial experiments revealed that ESCs cocultured with embryonic paw pad (EPP) tissue demonstrated glandular structure and cytokeratin 8 (K8) and cytokeratin 18 (K18) positive, while ESCs cocultured with embryonic dorsal skin demonstrated “sea snail” structure and K8, K18 negative. Moreover, bone morphogenetic protein 4 (BMP4) and epidermal growth factor (EGF) concentrations were detected in the medium of the EPP group. BMP receptor inhibitor could effectively block the ESC differentiation to sweat glands, while EGF receptor blocker did not show the effect. Our results showed clear benefits of this novel and convenient model in terms of in vitro-in vivo correlation. It was an appropriate alternative for screening of potential bioactives regulating the sweat gland morphogenesis mechanism.http://dx.doi.org/10.1155/2019/4254759 |
| spellingShingle | Tian Hu Yongde Xu Bin Yao Xiaobing Fu Sha Huang Developing a Novel and Convenient Model for Investigating Sweat Gland Morphogenesis from Epidermal Stem Cells Stem Cells International |
| title | Developing a Novel and Convenient Model for Investigating Sweat Gland Morphogenesis from Epidermal Stem Cells |
| title_full | Developing a Novel and Convenient Model for Investigating Sweat Gland Morphogenesis from Epidermal Stem Cells |
| title_fullStr | Developing a Novel and Convenient Model for Investigating Sweat Gland Morphogenesis from Epidermal Stem Cells |
| title_full_unstemmed | Developing a Novel and Convenient Model for Investigating Sweat Gland Morphogenesis from Epidermal Stem Cells |
| title_short | Developing a Novel and Convenient Model for Investigating Sweat Gland Morphogenesis from Epidermal Stem Cells |
| title_sort | developing a novel and convenient model for investigating sweat gland morphogenesis from epidermal stem cells |
| url | http://dx.doi.org/10.1155/2019/4254759 |
| work_keys_str_mv | AT tianhu developinganovelandconvenientmodelforinvestigatingsweatglandmorphogenesisfromepidermalstemcells AT yongdexu developinganovelandconvenientmodelforinvestigatingsweatglandmorphogenesisfromepidermalstemcells AT binyao developinganovelandconvenientmodelforinvestigatingsweatglandmorphogenesisfromepidermalstemcells AT xiaobingfu developinganovelandconvenientmodelforinvestigatingsweatglandmorphogenesisfromepidermalstemcells AT shahuang developinganovelandconvenientmodelforinvestigatingsweatglandmorphogenesisfromepidermalstemcells |