A Novel Kinetic Spectrophotometric Method for the Determination of Dopamine in Biological and Pharmaceutical Samples
A sensitive and accurate method for quantitative determination of dopamine was introduced. The proposed method is based on inhibitory effect of dopamine on the oxidation of thionine by bromate in acidic media. The change in absorbance was followed spectrophotometrically at 601 nm. The dependence of...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/819460 |
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| author | M. Reza Shishehbore Adel Asgharpoor N. Nasirizadeh |
| author_facet | M. Reza Shishehbore Adel Asgharpoor N. Nasirizadeh |
| author_sort | M. Reza Shishehbore |
| collection | DOAJ |
| description | A sensitive and accurate method for quantitative determination of dopamine was introduced. The proposed method is based on inhibitory effect of dopamine on the oxidation of thionine by bromate in acidic media. The change in absorbance was followed spectrophotometrically at 601 nm. The dependence of sensitivity on the reaction variables was investigated and optimized to obtain the maximum sensitivity. Under optimum experimental conditions, calibration curve was linear over the range 0.2–103.3 μg mL−1 of dopamine. The relative standard deviations () of 0.5, 1.0, 5.0, and 30.0 μg mL−1 of dopamine were 1.13, 1.02, 0.99, and 0.97%, respectively. The limit of detection was 0.057 μg mL−1 of dopamine. The effect of diverse species was also investigated. The developed method was successfully applied for the determination of dopamine in pharmaceutical and biological samples. |
| format | Article |
| id | doaj-art-7651da1a3d2744a789d27cda5f207fbf |
| institution | OA Journals |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-7651da1a3d2744a789d27cda5f207fbf2025-08-20T02:07:13ZengWileyJournal of Chemistry2090-90632090-90712013-01-01201310.1155/2013/819460819460A Novel Kinetic Spectrophotometric Method for the Determination of Dopamine in Biological and Pharmaceutical SamplesM. Reza Shishehbore0Adel Asgharpoor1N. Nasirizadeh2Department of Chemistry, Yazd Branch, Islamic Azad University, Yazd 8916871967, IranDepartment of Chemistry, Yazd Branch, Islamic Azad University, Yazd 8916871967, IranDepartment of Chemistry, Yazd Branch, Islamic Azad University, Yazd 8916871967, IranA sensitive and accurate method for quantitative determination of dopamine was introduced. The proposed method is based on inhibitory effect of dopamine on the oxidation of thionine by bromate in acidic media. The change in absorbance was followed spectrophotometrically at 601 nm. The dependence of sensitivity on the reaction variables was investigated and optimized to obtain the maximum sensitivity. Under optimum experimental conditions, calibration curve was linear over the range 0.2–103.3 μg mL−1 of dopamine. The relative standard deviations () of 0.5, 1.0, 5.0, and 30.0 μg mL−1 of dopamine were 1.13, 1.02, 0.99, and 0.97%, respectively. The limit of detection was 0.057 μg mL−1 of dopamine. The effect of diverse species was also investigated. The developed method was successfully applied for the determination of dopamine in pharmaceutical and biological samples.http://dx.doi.org/10.1155/2013/819460 |
| spellingShingle | M. Reza Shishehbore Adel Asgharpoor N. Nasirizadeh A Novel Kinetic Spectrophotometric Method for the Determination of Dopamine in Biological and Pharmaceutical Samples Journal of Chemistry |
| title | A Novel Kinetic Spectrophotometric Method for the Determination of Dopamine in Biological and Pharmaceutical Samples |
| title_full | A Novel Kinetic Spectrophotometric Method for the Determination of Dopamine in Biological and Pharmaceutical Samples |
| title_fullStr | A Novel Kinetic Spectrophotometric Method for the Determination of Dopamine in Biological and Pharmaceutical Samples |
| title_full_unstemmed | A Novel Kinetic Spectrophotometric Method for the Determination of Dopamine in Biological and Pharmaceutical Samples |
| title_short | A Novel Kinetic Spectrophotometric Method for the Determination of Dopamine in Biological and Pharmaceutical Samples |
| title_sort | novel kinetic spectrophotometric method for the determination of dopamine in biological and pharmaceutical samples |
| url | http://dx.doi.org/10.1155/2013/819460 |
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