Influence of cucumber mosaic virus loading by a synthesized jasmonic acid analogue in plant
Jasmonic acidis (Ja) is an endogenous regulators with ubiquitous distribution in plants. There are a variety of jasmonic acid analogues (JAs) with important functions in regulation of plant organ formation and many other phtophsiological processes, such as inhibition of germination of non-dormancy s...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2003-09-01
|
| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2003.05.0534 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Jasmonic acidis (Ja) is an endogenous regulators with ubiquitous distribution in plants. There are a variety of jasmonic acid analogues (JAs) with important functions in regulation of plant organ formation and many other phtophsiological processes, such as inhibition of germination of non-dormancy seeds, intimidation of plant root growth, stimulation of tuber formation etc. As a key chemical signal, JA is commonly involved in plant response to mechanical damage and pathogen infection. PDJ (9, 10-Propyl dihydro-jasmonate) is a kind of synthesized jasmonic acid, which has been used as a plant growth regulator. In this study, RNA spot hybridization was used to detect the replication of cucumber mosaic virus (CMV) on Nicandra physalodes plants, which were treated by different concentration of PDJ. Phosphor image laser scanner was used to quantify the result of viral RNA hybridization. Our result showed that the accumulation of CMV RNA3 in infected plant leaves was markedly influenced by PDJ treatment, and a time-and dose-dependent model was established. During the early stage of PDJ treatment (7d), the amount of CMV genomic RNA3 accumulated more rapidly than non-PDJ control. But PDJ treatment reduced the viral-RNA loading compared with the control as time proceeded. 21 days after treatment, the viral RNA3 loading in host leaves treated with 0.001-5 mg/kg PDJ was significantly lower than those untreated leaves, but loading in those treated with 50 mg/kg PDJ was still higher than in untreated ones. After 42 d treatment, the viral RNA3 loading in host leaves of the control was higher than all leaves treated with PDJ. The inhibition was enhanced with decreasing concentration of PDJ. |
|---|---|
| ISSN: | 1008-9209 2097-5155 |