Quantitative Ratio of Primer Pairs and Annealing Temperature Affecting PCR Products in Duplex Amplification
The quantity of PCR products that are simultaneously amplified from two different loci in a duplex amplification (DA) are significantly lower for one of the loci, as compared to identical PCR amplification in separate single-band amplifications (SBA). This difference in amplification probably occurs...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1999-10-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/99274st07 |
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