Recombinant in vitro test T-SPOT.TB as a screening method for early diagnosis of tuberculosis infection

In addition to standard skin tests (Mantoux test with 2 TU PPD-L and diaskintest) for the diagnosis of tuberculosis infection, in vitro tests are used. One of these tests is T-SPOT.TB being more widely used in recent years.The objective: to evaluate the effectiveness of T-SPOT.TB test for early dete...

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Main Authors: E. P. Eremenko, E. A. Borodulina, I. A. Sergeeva, D. A. Kudlay, B. E. Borodulin
Format: Article
Language:Russian
Published: New Terra Publishing House 2020-05-01
Series:Туберкулез и болезни лёгких
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Online Access:https://www.tibl-journal.com/jour/article/view/1417
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author E. P. Eremenko
E. A. Borodulina
I. A. Sergeeva
D. A. Kudlay
B. E. Borodulin
author_facet E. P. Eremenko
E. A. Borodulina
I. A. Sergeeva
D. A. Kudlay
B. E. Borodulin
author_sort E. P. Eremenko
collection DOAJ
description In addition to standard skin tests (Mantoux test with 2 TU PPD-L and diaskintest) for the diagnosis of tuberculosis infection, in vitro tests are used. One of these tests is T-SPOT.TB being more widely used in recent years.The objective: to evaluate the effectiveness of T-SPOT.TB test for early detection of tuberculosis infection in children and adolescents in Samara Region.Subjects and methods. From 2016 to 2019, results of T-SPOT.TB tests performed in 596 children aged 2 to 17 years inclusive were analyzed; those children had no immunodiagnosis of tuberculosis infection using skin tests since their parents refused to have it.Results. It was found out that the major reason for refusing skin tests was the “fear” of visiting a TB dispensary if the result had been positive — 38.43% (n = 229). The latent tuberculosis infection according to the results of T-SPOT.TB among children with concomitant pathology made 2.6%, among healthy children – 0.7%.Conclusion. T-SPOT.TB test may be used as an alternative method for diagnosis of tuberculosis infection, should the parent refuse to have skin tests. In children with concomitant pathology, T-SPOT.TB test can serve as a leading method for immunodiagnosis of tuberculosis.The authors state that they have no conflict of interests.
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series Туберкулез и болезни лёгких
spelling doaj-art-7632af605caf46db93fbce54f62f76092025-08-20T01:47:52ZrusNew Terra Publishing HouseТуберкулез и болезни лёгких2075-12302542-15062020-05-01984485210.21292/2075-1230-2020-98-4-48-521416Recombinant in vitro test T-SPOT.TB as a screening method for early diagnosis of tuberculosis infectionE. P. Eremenko0E. A. Borodulina1I. A. Sergeeva2D. A. Kudlay3B. E. Borodulin4Samara State Medical UniversitySamara State Medical UniversityMother and Child, Pediatric Polyclinic, IDK Private Medical CenterImmunology Research Institute by the Federal Medical Biological AgencySamara State Medical UniversityIn addition to standard skin tests (Mantoux test with 2 TU PPD-L and diaskintest) for the diagnosis of tuberculosis infection, in vitro tests are used. One of these tests is T-SPOT.TB being more widely used in recent years.The objective: to evaluate the effectiveness of T-SPOT.TB test for early detection of tuberculosis infection in children and adolescents in Samara Region.Subjects and methods. From 2016 to 2019, results of T-SPOT.TB tests performed in 596 children aged 2 to 17 years inclusive were analyzed; those children had no immunodiagnosis of tuberculosis infection using skin tests since their parents refused to have it.Results. It was found out that the major reason for refusing skin tests was the “fear” of visiting a TB dispensary if the result had been positive — 38.43% (n = 229). The latent tuberculosis infection according to the results of T-SPOT.TB among children with concomitant pathology made 2.6%, among healthy children – 0.7%.Conclusion. T-SPOT.TB test may be used as an alternative method for diagnosis of tuberculosis infection, should the parent refuse to have skin tests. In children with concomitant pathology, T-SPOT.TB test can serve as a leading method for immunodiagnosis of tuberculosis.The authors state that they have no conflict of interests.https://www.tibl-journal.com/jour/article/view/1417mantoux testdiaskintesttuberculous recombinant allergent-spot.tblatent tuberculosis infection
spellingShingle E. P. Eremenko
E. A. Borodulina
I. A. Sergeeva
D. A. Kudlay
B. E. Borodulin
Recombinant in vitro test T-SPOT.TB as a screening method for early diagnosis of tuberculosis infection
Туберкулез и болезни лёгких
mantoux test
diaskintest
tuberculous recombinant allergen
t-spot.tb
latent tuberculosis infection
title Recombinant in vitro test T-SPOT.TB as a screening method for early diagnosis of tuberculosis infection
title_full Recombinant in vitro test T-SPOT.TB as a screening method for early diagnosis of tuberculosis infection
title_fullStr Recombinant in vitro test T-SPOT.TB as a screening method for early diagnosis of tuberculosis infection
title_full_unstemmed Recombinant in vitro test T-SPOT.TB as a screening method for early diagnosis of tuberculosis infection
title_short Recombinant in vitro test T-SPOT.TB as a screening method for early diagnosis of tuberculosis infection
title_sort recombinant in vitro test t spot tb as a screening method for early diagnosis of tuberculosis infection
topic mantoux test
diaskintest
tuberculous recombinant allergen
t-spot.tb
latent tuberculosis infection
url https://www.tibl-journal.com/jour/article/view/1417
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AT iasergeeva recombinantinvitrotesttspottbasascreeningmethodforearlydiagnosisoftuberculosisinfection
AT dakudlay recombinantinvitrotesttspottbasascreeningmethodforearlydiagnosisoftuberculosisinfection
AT beborodulin recombinantinvitrotesttspottbasascreeningmethodforearlydiagnosisoftuberculosisinfection