Structural characteristics and antioxidant activity of a low-molecular-weight jujube polysaccharide by ultrasound assisted metal-free Fenton reaction
This study used an ultrasonically accelerated metal-free Fenton (H2O2-Vc system) reaction to promote water-extracted degrading polysaccharides from Ziziphus Jujuba cv. Muzao (DZMP). A novel jujube polysaccharide (DPZMP3) was obtained by degradation using DEAE-Sepharose Fast Flow and Sephacryl S-100...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-12-01
|
| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S259015752400796X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850109154207727616 |
|---|---|
| author | Yingying Liu Yan Meng Haozhen Ji Jianhang Guo Miaomiao Shi Feiliao Lai Xiaolong Ji |
| author_facet | Yingying Liu Yan Meng Haozhen Ji Jianhang Guo Miaomiao Shi Feiliao Lai Xiaolong Ji |
| author_sort | Yingying Liu |
| collection | DOAJ |
| description | This study used an ultrasonically accelerated metal-free Fenton (H2O2-Vc system) reaction to promote water-extracted degrading polysaccharides from Ziziphus Jujuba cv. Muzao (DZMP). A novel jujube polysaccharide (DPZMP3) was obtained by degradation using DEAE-Sepharose Fast Flow and Sephacryl S-100 column chromatography. Methylation analysis, HPGPC, ion chromatography, FT-IR, and NMR spectroscopies were used to clarify the chemical structures of DPZMP3. Monosaccharide compositional analysis of DPZMP3 revealed the presence of Rha, Ara, Gal, and GalA at a molar ratio of 1.00:1.49:1.60:7.68, and the HPGPC data demonstrated the average Mw of 34.3 kDa. Based on the structural and linkage research using NMR spectroscopy and GC–MS, it was determined that DPZMP3 was a homogalacturonan pectic polysaccharide with a (1 → 4)-Galp branch at C-6 and a small amount of Araf and Rhap residues. The ultrasonic-aided Fenton treatment did not significantly alter the structure of DPZMP3. It may also be useful for DZMP and enhancing their antioxidant activity in vitro. The current study's findings could pave the way for the food sector to use jujube polysaccharides obtained by degradation as a functional food component. |
| format | Article |
| id | doaj-art-762cfe562c1d43f3bbab249a694f6ee0 |
| institution | OA Journals |
| issn | 2590-1575 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-762cfe562c1d43f3bbab249a694f6ee02025-08-20T02:38:10ZengElsevierFood Chemistry: X2590-15752024-12-012410190810.1016/j.fochx.2024.101908Structural characteristics and antioxidant activity of a low-molecular-weight jujube polysaccharide by ultrasound assisted metal-free Fenton reactionYingying Liu0Yan Meng1Haozhen Ji2Jianhang Guo3Miaomiao Shi4Feiliao Lai5Xiaolong Ji6College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China; National & Local Joint Engineering Research Center of Cereal-Based Foods (Henan), Zhengzhou 450001, China; Key Laboratory of Cold Chain Food Processing and Safety Control, Ministry of Education, Zhengzhou University of Light Industry, Zhengzhou 450001, ChinaSchool of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, ChinaCollege of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China; National & Local Joint Engineering Research Center of Cereal-Based Foods (Henan), Zhengzhou 450001, ChinaCollege of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China; National & Local Joint Engineering Research Center of Cereal-Based Foods (Henan), Zhengzhou 450001, ChinaCollege of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China; National & Local Joint Engineering Research Center of Cereal-Based Foods (Henan), Zhengzhou 450001, ChinaDepartment of Physics, School of Science, Tianjin University, Tianjin 300072, China; Corresponding author at: Department of Physics, School of Science, Tianjin University, China.College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China; National & Local Joint Engineering Research Center of Cereal-Based Foods (Henan), Zhengzhou 450001, China; Key Laboratory of Cold Chain Food Processing and Safety Control, Ministry of Education, Zhengzhou University of Light Industry, Zhengzhou 450001, China; Corresponding author at: College of Food and Bioengineering, Zhengzhou University of Light Industry, China.This study used an ultrasonically accelerated metal-free Fenton (H2O2-Vc system) reaction to promote water-extracted degrading polysaccharides from Ziziphus Jujuba cv. Muzao (DZMP). A novel jujube polysaccharide (DPZMP3) was obtained by degradation using DEAE-Sepharose Fast Flow and Sephacryl S-100 column chromatography. Methylation analysis, HPGPC, ion chromatography, FT-IR, and NMR spectroscopies were used to clarify the chemical structures of DPZMP3. Monosaccharide compositional analysis of DPZMP3 revealed the presence of Rha, Ara, Gal, and GalA at a molar ratio of 1.00:1.49:1.60:7.68, and the HPGPC data demonstrated the average Mw of 34.3 kDa. Based on the structural and linkage research using NMR spectroscopy and GC–MS, it was determined that DPZMP3 was a homogalacturonan pectic polysaccharide with a (1 → 4)-Galp branch at C-6 and a small amount of Araf and Rhap residues. The ultrasonic-aided Fenton treatment did not significantly alter the structure of DPZMP3. It may also be useful for DZMP and enhancing their antioxidant activity in vitro. The current study's findings could pave the way for the food sector to use jujube polysaccharides obtained by degradation as a functional food component.http://www.sciencedirect.com/science/article/pii/S259015752400796XUltrasound assisted H2O2/VC reactionJujube polysaccharideStructureAntioxidant activity |
| spellingShingle | Yingying Liu Yan Meng Haozhen Ji Jianhang Guo Miaomiao Shi Feiliao Lai Xiaolong Ji Structural characteristics and antioxidant activity of a low-molecular-weight jujube polysaccharide by ultrasound assisted metal-free Fenton reaction Food Chemistry: X Ultrasound assisted H2O2/VC reaction Jujube polysaccharide Structure Antioxidant activity |
| title | Structural characteristics and antioxidant activity of a low-molecular-weight jujube polysaccharide by ultrasound assisted metal-free Fenton reaction |
| title_full | Structural characteristics and antioxidant activity of a low-molecular-weight jujube polysaccharide by ultrasound assisted metal-free Fenton reaction |
| title_fullStr | Structural characteristics and antioxidant activity of a low-molecular-weight jujube polysaccharide by ultrasound assisted metal-free Fenton reaction |
| title_full_unstemmed | Structural characteristics and antioxidant activity of a low-molecular-weight jujube polysaccharide by ultrasound assisted metal-free Fenton reaction |
| title_short | Structural characteristics and antioxidant activity of a low-molecular-weight jujube polysaccharide by ultrasound assisted metal-free Fenton reaction |
| title_sort | structural characteristics and antioxidant activity of a low molecular weight jujube polysaccharide by ultrasound assisted metal free fenton reaction |
| topic | Ultrasound assisted H2O2/VC reaction Jujube polysaccharide Structure Antioxidant activity |
| url | http://www.sciencedirect.com/science/article/pii/S259015752400796X |
| work_keys_str_mv | AT yingyingliu structuralcharacteristicsandantioxidantactivityofalowmolecularweightjujubepolysaccharidebyultrasoundassistedmetalfreefentonreaction AT yanmeng structuralcharacteristicsandantioxidantactivityofalowmolecularweightjujubepolysaccharidebyultrasoundassistedmetalfreefentonreaction AT haozhenji structuralcharacteristicsandantioxidantactivityofalowmolecularweightjujubepolysaccharidebyultrasoundassistedmetalfreefentonreaction AT jianhangguo structuralcharacteristicsandantioxidantactivityofalowmolecularweightjujubepolysaccharidebyultrasoundassistedmetalfreefentonreaction AT miaomiaoshi structuralcharacteristicsandantioxidantactivityofalowmolecularweightjujubepolysaccharidebyultrasoundassistedmetalfreefentonreaction AT feiliaolai structuralcharacteristicsandantioxidantactivityofalowmolecularweightjujubepolysaccharidebyultrasoundassistedmetalfreefentonreaction AT xiaolongji structuralcharacteristicsandantioxidantactivityofalowmolecularweightjujubepolysaccharidebyultrasoundassistedmetalfreefentonreaction |