A Highly Selective Fluorescent Probe for Hypochlorous Acid in Living Cells Based on a Naphthalene Derivative
Hypochlorous acid (HOCl) was crucial for maintaining the homeostasis in cells and plays vital roles in many physiological and pathological processes. In this work, a highly selective fluorescent probe for hypochlorous acid in living cells was constructed and prepared based on a naphthalene derivativ...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2022/7649230 |
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| author | Jingguo Sun Junhong Xu Qiujuan Ma Guojiang Mao Nannan Zhu Meiju Tian Linke Li Shuzhen Liu |
| author_facet | Jingguo Sun Junhong Xu Qiujuan Ma Guojiang Mao Nannan Zhu Meiju Tian Linke Li Shuzhen Liu |
| author_sort | Jingguo Sun |
| collection | DOAJ |
| description | Hypochlorous acid (HOCl) was crucial for maintaining the homeostasis in cells and plays vital roles in many physiological and pathological processes. In this work, a highly selective fluorescent probe for hypochlorous acid in living cells was constructed and prepared based on a naphthalene derivative. A naphthalene derivative was utilized as the fluorescent group, and N,N-dimethylthiocarbamate was applied as the selective recognition site for HOCl. Before adding HOCl, the fluorescent probe exhibited weak fluorescence. Upon adding HOCl, the fluorescent probe displayed remarkable fluorescence enhancement. The fluorescence intensity at 502 nm showed a linear response to the concentration of HOCl from 3.0 × 10−7 to 1.0 × 10−5 mol·L−1. The detection limit was estimated to be 1.5 × 10−7 mol·L−1 for HOCl. The fluorescent probe showed fast response and outstanding selectivity toward HOCl. It owned good biocompatibility and had also been successfully applied in the confocal imaging of exogenous and endogenous HOCl in living cells. |
| format | Article |
| id | doaj-art-762558eed2eb4af8b943e7dc8f028a47 |
| institution | Kabale University |
| issn | 1687-8779 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-762558eed2eb4af8b943e7dc8f028a472025-02-03T06:13:31ZengWileyInternational Journal of Analytical Chemistry1687-87792022-01-01202210.1155/2022/7649230A Highly Selective Fluorescent Probe for Hypochlorous Acid in Living Cells Based on a Naphthalene DerivativeJingguo Sun0Junhong Xu1Qiujuan Ma2Guojiang Mao3Nannan Zhu4Meiju Tian5Linke Li6Shuzhen Liu7School of PharmacyDepartment of Dynamical EngineeringSchool of PharmacyHenan Key Laboratory of Organic Functional Molecule and Drug InnovationSchool of PharmacySchool of PharmacySchool of PharmacySchool of PharmacyHypochlorous acid (HOCl) was crucial for maintaining the homeostasis in cells and plays vital roles in many physiological and pathological processes. In this work, a highly selective fluorescent probe for hypochlorous acid in living cells was constructed and prepared based on a naphthalene derivative. A naphthalene derivative was utilized as the fluorescent group, and N,N-dimethylthiocarbamate was applied as the selective recognition site for HOCl. Before adding HOCl, the fluorescent probe exhibited weak fluorescence. Upon adding HOCl, the fluorescent probe displayed remarkable fluorescence enhancement. The fluorescence intensity at 502 nm showed a linear response to the concentration of HOCl from 3.0 × 10−7 to 1.0 × 10−5 mol·L−1. The detection limit was estimated to be 1.5 × 10−7 mol·L−1 for HOCl. The fluorescent probe showed fast response and outstanding selectivity toward HOCl. It owned good biocompatibility and had also been successfully applied in the confocal imaging of exogenous and endogenous HOCl in living cells.http://dx.doi.org/10.1155/2022/7649230 |
| spellingShingle | Jingguo Sun Junhong Xu Qiujuan Ma Guojiang Mao Nannan Zhu Meiju Tian Linke Li Shuzhen Liu A Highly Selective Fluorescent Probe for Hypochlorous Acid in Living Cells Based on a Naphthalene Derivative International Journal of Analytical Chemistry |
| title | A Highly Selective Fluorescent Probe for Hypochlorous Acid in Living Cells Based on a Naphthalene Derivative |
| title_full | A Highly Selective Fluorescent Probe for Hypochlorous Acid in Living Cells Based on a Naphthalene Derivative |
| title_fullStr | A Highly Selective Fluorescent Probe for Hypochlorous Acid in Living Cells Based on a Naphthalene Derivative |
| title_full_unstemmed | A Highly Selective Fluorescent Probe for Hypochlorous Acid in Living Cells Based on a Naphthalene Derivative |
| title_short | A Highly Selective Fluorescent Probe for Hypochlorous Acid in Living Cells Based on a Naphthalene Derivative |
| title_sort | highly selective fluorescent probe for hypochlorous acid in living cells based on a naphthalene derivative |
| url | http://dx.doi.org/10.1155/2022/7649230 |
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