Periosteum-Derived Mesenchymal Stem Cells Secretome - Cell-Free Strategy for Endogenous Bone Regeneration: Proteomic Analysis in Vitro
Objectives: Millions of people worldwide are affected by diseases or injuries which lead to bone/tooth loss and defects. While such clinical situations are daily practice in most of the hospitals, the widely used treatment methods still have disadvantages. Therefore, this field of medicine is active...
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| Format: | Article |
| Language: | English |
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Lithuanian University of Health Sciences, Faculty of Odontology
2021-12-01
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| Series: | eJournal of Oral Maxillofacial Research |
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| Online Access: | https://www.ejomr.org/JOMR/archives/2021/2/e2/v12n2e2ht.htm |
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| _version_ | 1849251120409477120 |
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| author | Mindaugas Pranskunas Egidijus Simoliunas Milda Alksne Algirdas Kaupinis Gintaras Juodzbalys |
| author_facet | Mindaugas Pranskunas Egidijus Simoliunas Milda Alksne Algirdas Kaupinis Gintaras Juodzbalys |
| author_sort | Mindaugas Pranskunas |
| collection | DOAJ |
| description | Objectives: Millions of people worldwide are affected by diseases or injuries which lead to bone/tooth loss and defects. While such clinical situations are daily practice in most of the hospitals, the widely used treatment methods still have disadvantages. Therefore, this field of medicine is actively searching new tissue regeneration techniques, one of which could be stem cell secretome. Thus, the purpose of this research study was to perform the detail proteomic analysis of periosteum-derived mesenchymal stem cells secretome in order to evaluate if it is capable to induce osteo-regenerative process.
Material and Methods: Periosteum-derived mesenchymal stem cells (PMSCs) were extracted from adult male New Zealand White rabbits. Cells were characterised by evaluating their differentiation potential. After characterisation PMSCs secretomes were collected and their proteomic analysis was performed.
Results: PMSCs were extracted from adult male New Zealand White rabbits. In order to characterise the extracted PMSCs, they were differentiated in the directions which mainly describes MSC multipotency - osteogenic, myogenic and adipogenic. A total of 146 proteins were detected. After characterisation PMSCs secretomes were collected and their proteomic analysis was performed. The resulting protein composition indicates the ability to promote bone regeneration to fully mature bone.
Conclusions: Bioactive molecules detected in periosteum-derived mesenchymal stem cells secretome initiates the processes required for the formation of a fully functional bone. |
| format | Article |
| id | doaj-art-7621ef0d10d94ee2872df94c1f689320 |
| institution | Kabale University |
| issn | 2029-283X |
| language | English |
| publishDate | 2021-12-01 |
| publisher | Lithuanian University of Health Sciences, Faculty of Odontology |
| record_format | Article |
| series | eJournal of Oral Maxillofacial Research |
| spelling | doaj-art-7621ef0d10d94ee2872df94c1f6893202025-08-20T03:57:03ZengLithuanian University of Health Sciences, Faculty of OdontologyeJournal of Oral Maxillofacial Research2029-283X2021-12-01122e210.5037/jomr.2021.12202Periosteum-Derived Mesenchymal Stem Cells Secretome - Cell-Free Strategy for Endogenous Bone Regeneration: Proteomic Analysis in VitroMindaugas PranskunasEgidijus SimoliunasMilda AlksneAlgirdas KaupinisGintaras JuodzbalysObjectives: Millions of people worldwide are affected by diseases or injuries which lead to bone/tooth loss and defects. While such clinical situations are daily practice in most of the hospitals, the widely used treatment methods still have disadvantages. Therefore, this field of medicine is actively searching new tissue regeneration techniques, one of which could be stem cell secretome. Thus, the purpose of this research study was to perform the detail proteomic analysis of periosteum-derived mesenchymal stem cells secretome in order to evaluate if it is capable to induce osteo-regenerative process. Material and Methods: Periosteum-derived mesenchymal stem cells (PMSCs) were extracted from adult male New Zealand White rabbits. Cells were characterised by evaluating their differentiation potential. After characterisation PMSCs secretomes were collected and their proteomic analysis was performed. Results: PMSCs were extracted from adult male New Zealand White rabbits. In order to characterise the extracted PMSCs, they were differentiated in the directions which mainly describes MSC multipotency - osteogenic, myogenic and adipogenic. A total of 146 proteins were detected. After characterisation PMSCs secretomes were collected and their proteomic analysis was performed. The resulting protein composition indicates the ability to promote bone regeneration to fully mature bone. Conclusions: Bioactive molecules detected in periosteum-derived mesenchymal stem cells secretome initiates the processes required for the formation of a fully functional bone.https://www.ejomr.org/JOMR/archives/2021/2/e2/v12n2e2ht.htmbone regenerationmesenchymal stem cellsperiosteum |
| spellingShingle | Mindaugas Pranskunas Egidijus Simoliunas Milda Alksne Algirdas Kaupinis Gintaras Juodzbalys Periosteum-Derived Mesenchymal Stem Cells Secretome - Cell-Free Strategy for Endogenous Bone Regeneration: Proteomic Analysis in Vitro eJournal of Oral Maxillofacial Research bone regeneration mesenchymal stem cells periosteum |
| title | Periosteum-Derived Mesenchymal Stem Cells Secretome - Cell-Free Strategy for Endogenous Bone Regeneration: Proteomic Analysis in Vitro |
| title_full | Periosteum-Derived Mesenchymal Stem Cells Secretome - Cell-Free Strategy for Endogenous Bone Regeneration: Proteomic Analysis in Vitro |
| title_fullStr | Periosteum-Derived Mesenchymal Stem Cells Secretome - Cell-Free Strategy for Endogenous Bone Regeneration: Proteomic Analysis in Vitro |
| title_full_unstemmed | Periosteum-Derived Mesenchymal Stem Cells Secretome - Cell-Free Strategy for Endogenous Bone Regeneration: Proteomic Analysis in Vitro |
| title_short | Periosteum-Derived Mesenchymal Stem Cells Secretome - Cell-Free Strategy for Endogenous Bone Regeneration: Proteomic Analysis in Vitro |
| title_sort | periosteum derived mesenchymal stem cells secretome cell free strategy for endogenous bone regeneration proteomic analysis in vitro |
| topic | bone regeneration mesenchymal stem cells periosteum |
| url | https://www.ejomr.org/JOMR/archives/2021/2/e2/v12n2e2ht.htm |
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