β-suppressor protein 1 (ARRB1)-△exon13 modulates the progression of glioblastoma via combination with glycolysis-related proteins
Glioblastoma multiform (GBM) constitutes approximately 14.7 % of all central nervous system tumors (CNSTs) and 45.2 % of primary malignant CNSTs. Extensive research has indicated that β-arrestin 1 (ARRB1) plays a significant role in tumor malignancy. In this investigation, we established GBM cell li...
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Elsevier
2025-06-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580825001359 |
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| author | Zi-Long Wei Shuo Han Dong-Hua Han Xue-Tao Li Yu-Lun Huang Zhi-Min Wang |
| author_facet | Zi-Long Wei Shuo Han Dong-Hua Han Xue-Tao Li Yu-Lun Huang Zhi-Min Wang |
| author_sort | Zi-Long Wei |
| collection | DOAJ |
| description | Glioblastoma multiform (GBM) constitutes approximately 14.7 % of all central nervous system tumors (CNSTs) and 45.2 % of primary malignant CNSTs. Extensive research has indicated that β-arrestin 1 (ARRB1) plays a significant role in tumor malignancy. In this investigation, we established GBM cell lines representing normal control (NC), overexpression (OE) and Δexon13 GBM variants (△exon13) of ARRB1. Our findings indicate that the ARRB1-OE isoform facilitated GBM cell proliferation and migration, with the ARRB1-△exon13 isoform further augmenting this effect. Notably, the isoform ARRB1-△exon13 binds to glycolytic proteins including ENO1 and ALDOA and regulates glycolysis. In vivo studies corroborate the tumor-promoting effects of ARRB1-Δexon13. Furthermore, we demonstrate that 2-DG effectively inhibits the malignancy-promoting capabilities of ARRB1-Δexon13 by reducing pyruvate levels. Our identification of alternative RNA splicing events of ARRB1 reveals a mechanism by which GBM cell malignancy is augmented through ARRB1-Δexon13, which mediates glycolysis-related pathways. |
| format | Article |
| id | doaj-art-761e22d5f5724ca8a3df70abbdfa2a32 |
| institution | OA Journals |
| issn | 2405-5808 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj-art-761e22d5f5724ca8a3df70abbdfa2a322025-08-20T02:37:46ZengElsevierBiochemistry and Biophysics Reports2405-58082025-06-014210204810.1016/j.bbrep.2025.102048β-suppressor protein 1 (ARRB1)-△exon13 modulates the progression of glioblastoma via combination with glycolysis-related proteinsZi-Long Wei0Shuo Han1Dong-Hua Han2Xue-Tao Li3Yu-Lun Huang4Zhi-Min Wang5Department of Neurosurgery, The Fourth Hospital Affiliated to Soochow University, Suzhou Dushu Lake Hospital, Suzhou, 215000, China; Department of Neurosurgery, Shanghai Pudong Hospital Affliated to Fudan University, Pudong Medical Center, Shanghai, 201399, ChinaDepartment of Neurosurgery, Shanghai Changzheng Hospital, Shanghai, ChinaDepartment of Neurosurgery, Shanghai Pudong Hospital Affliated to Fudan University, Pudong Medical Center, Shanghai, 201399, ChinaDepartment of Neurosurgery, The Fourth Hospital Affiliated to Soochow University, Suzhou Dushu Lake Hospital, Suzhou, 215000, ChinaDepartment of Neurosurgery, The Fourth Hospital Affiliated to Soochow University, Suzhou Dushu Lake Hospital, Suzhou, 215000, ChinaDepartment of Neurosurgery, The Fourth Hospital Affiliated to Soochow University, Suzhou Dushu Lake Hospital, Suzhou, 215000, China; Corresponding author. No. 9 Chongwen Road, Suzhou Industrial Park, Jiangsu, China.Glioblastoma multiform (GBM) constitutes approximately 14.7 % of all central nervous system tumors (CNSTs) and 45.2 % of primary malignant CNSTs. Extensive research has indicated that β-arrestin 1 (ARRB1) plays a significant role in tumor malignancy. In this investigation, we established GBM cell lines representing normal control (NC), overexpression (OE) and Δexon13 GBM variants (△exon13) of ARRB1. Our findings indicate that the ARRB1-OE isoform facilitated GBM cell proliferation and migration, with the ARRB1-△exon13 isoform further augmenting this effect. Notably, the isoform ARRB1-△exon13 binds to glycolytic proteins including ENO1 and ALDOA and regulates glycolysis. In vivo studies corroborate the tumor-promoting effects of ARRB1-Δexon13. Furthermore, we demonstrate that 2-DG effectively inhibits the malignancy-promoting capabilities of ARRB1-Δexon13 by reducing pyruvate levels. Our identification of alternative RNA splicing events of ARRB1 reveals a mechanism by which GBM cell malignancy is augmented through ARRB1-Δexon13, which mediates glycolysis-related pathways.http://www.sciencedirect.com/science/article/pii/S2405580825001359GlioblastomaARRB1Alternative spicingGlycolysisMalignancy |
| spellingShingle | Zi-Long Wei Shuo Han Dong-Hua Han Xue-Tao Li Yu-Lun Huang Zhi-Min Wang β-suppressor protein 1 (ARRB1)-△exon13 modulates the progression of glioblastoma via combination with glycolysis-related proteins Biochemistry and Biophysics Reports Glioblastoma ARRB1 Alternative spicing Glycolysis Malignancy |
| title | β-suppressor protein 1 (ARRB1)-△exon13 modulates the progression of glioblastoma via combination with glycolysis-related proteins |
| title_full | β-suppressor protein 1 (ARRB1)-△exon13 modulates the progression of glioblastoma via combination with glycolysis-related proteins |
| title_fullStr | β-suppressor protein 1 (ARRB1)-△exon13 modulates the progression of glioblastoma via combination with glycolysis-related proteins |
| title_full_unstemmed | β-suppressor protein 1 (ARRB1)-△exon13 modulates the progression of glioblastoma via combination with glycolysis-related proteins |
| title_short | β-suppressor protein 1 (ARRB1)-△exon13 modulates the progression of glioblastoma via combination with glycolysis-related proteins |
| title_sort | β suppressor protein 1 arrb1 △exon13 modulates the progression of glioblastoma via combination with glycolysis related proteins |
| topic | Glioblastoma ARRB1 Alternative spicing Glycolysis Malignancy |
| url | http://www.sciencedirect.com/science/article/pii/S2405580825001359 |
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